Proteomic analysis of adult Galeruca daurica (Coleoptera: Chrysomelidae) at different stages during summer diapause

Identification and Comparative Profiling of microRNAs at Different Diapause Stages of Galeruca Daurica Adults

“…In the present study, the down-regulated differentially expressed miRNAs (20) were much more than the up-regulated differentially expressed miRNAs (5) during diapause (D/PD), which was in accord with our iTRAQ proteome data that the differentially overexpressed proteins (82) were more than the differentially underexpressed proteins (57) during diapause [30], because miRNA abundance has most often been negatively correlated with expression of its target genes. Reynolds et al also got similar results that the majority of differentially expressed miRNAs (8) were down-regulated during diapause and only two miRNAs were up-regulated in diapausing pupae than in their non-diapausing counterparts in S. bullata [3].…”

“…In this study, the 51 putative target unigenes involved in Ca 2+ signaling for 41 DEMs were obtained (Table S10). Our proteomic and qRT-PCR results showed that several CaPBs (calreticulin, calmodulin, troponin, and calcyphosine-like) were differentially expressed at the protein and mRNA levels during summer diapause of G. daurica [30,61]. The main miRNAs targeting these CaBPs included tca-miR-71-3p, bmo-mir-6497-p5_1ss4AG, tca-miR-2788-5p, tca-miR-2944c-3p_R+1, tca-miR-87b-3p_R-2, aga-miR-2944b-5p_L-1_1ss12CT, ame-miR-3759-3p_L-4R-2_1ss14TC, bmo-miR-317-3p_L-2R+1, pca-miR-980-3p_R+3, tca-miR-2944c-5p_L-1_1ss10TC, aae-miR-970_1ss20AG, bmo-miR-9a-5p_R-2, tca-miR-2765-5p_R+1, and PC-3p-32095_277.…”

“…The transcript encoding juvenile hormone epoxide hydrolase (JHEH) was signi cantly increased during adult diapause in Coccinella septempunctata [45]. Our proteomic analysis found that the juvenile hormone binding protein (JHBP) levels increased signi cantly in G. daurica adults developing from the PD to TD stages, whereas the juvenile hormone esterase (JHE) levels decreased [30]. qRT-PCR indicated that GdJHBP was expressed in various developmental stages of G. daurica with the highest expression in larvae and the lowest in eggs and pupae.…”

“…Galeruca daurica (Coleoptera: Chrysomelidae) is a new pest on the grasslands of Inner Mongolia, China. Since its sudden outbreak in 2009, this pest has continually spread and caused great losses to pasture on the Inner Mongolia grasslands [30,31]. The leaf beetle occurs one generation each year, and adults survive summer as an obligatory diapause form.…”

“…Chen et al investigated the biochemical change in G. daurica adults during oversummering [32]. Ma et al compared the change of proteomic levels in adults at the pre-diapause, diapause and post-diapause stages using iTRAQ, and found a large number of differentially-expressed proteins involved in metabolic process, stress response, cytoskeletal reorganization, and phagosome pathway [30]. Moreover, juvenile hormone regulation and Ca 2+ signaling may play an important role in the regulation of summer diapause in G. daurica.…”