Proteomic analysis of adipocyte differentiation: Evidence that α2 macroglobulin is involved in the adipose conversion of 3T3 L1 preadipocytes

Choi, Kai Luk; Wang, Yudong; Tse, Cynthia A.; Lam, Karen S.L.; Cooper, Garth J. S.; Xu, Aimin

doi:10.1002/pmic.200300697

Cited by 32 publications

(34 citation statements)

References 40 publications

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“…In fact, as it will be discussed later on and as shown in Table 2, there are numerous examples of the to beneficial effects that the ASC's secretome exerts in the central nervous systems (CNS), immune system, heart, muscle and even in general cell survival. There has been an extensive literature [37][38][39][40][41][42] dealing with the secretory profile of pre-adipocytes, ASCs, or adipose tissue as determined by Enzyme Linked Immunoabsorbent Assay (ELISA) or related techniques. Nevertheless, the profile of the secretory products of ASCs has only recently begun to be characterized at the mass spectrometry level.…”

Section: Ascs Secretome -Basics and Possible Appli-cationsmentioning

confidence: 99%

Adipose Tissue Derived Stem Cells Secretome: Soluble Factors and Their Roles in Regenerative Medicine

Salgado

Reis²,

Sousa³

et al. 2010

CSCR

520

217

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Stem cells have been long looked at as possible therapeutic vehicles for different health related problems. Among the different existing stem cell populations, Adipose derived Stem Cells (ASCs) have been gathering attention in the last 10 years. When compared to other stem cells populations and sources, ASCs can be easily isolated while providing higher yields upon the processing of adipose tissue. Similar to other stem cell populations, it was initially thought that the main potential of ASCs for regenerative medicine approaches was intimately related to their differentiation capability. Although this is true, there has been an increasing body of literature describing the trophic effects of ASCs on the protection, survival and differentiation of a variety of endogenous cells/tissues. Moreover, they have also shown to possess an immunomodulatory character. This effect is closely related to the ASCs' secretome and the soluble factors found within it. Molecules such as hepatocyte growth factor (HGF), granulocyte and macrophage colony stimulating factors, interleukins (ILs) 6, 7, 8 and 11, tumor necrosis factor-(TNF-), vascular endothelial growth factor (VEGF), brain derived neurotrophic factor (BDNF), nerve growth factor (NGF), adipokines and others have been identified within the ASCs' secretome. Due to its importance regarding future applications for the field of regenerative medicine, we aim, in the present review, to make a comprehensive analysis of the literature relating to the ASCs' secretome and its relevance to the immune and central nervous system, vascularization and cardiac regeneration. The concluding section will highlight some of the major challenges that remain before ASCs can be used for future clinical applications.

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Section: Ascs Secretome -Basics and Possible Appli-cationsmentioning

confidence: 99%

Adipose Tissue Derived Stem Cells Secretome: Soluble Factors and Their Roles in Regenerative Medicine

Salgado

Reis²,

Sousa³

et al. 2010

CSCR

520

217

View full text Add to dashboard Cite

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“…Pulse-chase experiments and 2-DE has been taken to study protein turnover in adipocyte-derived cell cultures [180], skeletal muscle-derived cells in culture [181] and in articular cartilage proteins [182].…”

Section: Proteome Turnovermentioning

confidence: 99%

Two‐dimensional gel electrophoresis and mass spectrometry for biomarker discovery

Penque

2009

Proteomics Clinical Apps

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The proteome project, initiated in 1995, was made possible by 2-DE combined with MS. The project main objective was and remains the identification of all proteins expressed by a cell, tissue or organism in a given time and condition. Following this objective, the global profiling of proteins in health versus pathological state by the 2-DE/MS-based proteomic approach has contributed to the elucidation of the basic mechanisms of disease by discovering candidate disease biomarkers and disease targets for new drug development. This review will briefly summarize the historical evolution of 2-DE up to today, and review 2-DE/MS technology and its specific methods of study of immunoresponse (immunoproteomics), PTM of proteins, complex proteinprotein interactions (interactome), the proteome of cell membrane and intracellular proteome turnover in disease biomarker discovery. 2-DE historical evolutionGlobal profiling of proteins in healthy versus pathological states is a strategy to discover biomarkers for early diagnostics, disease progression, treatment response and novel targets for therapeutic drugs development. The idea of making an inventory of all the proteins in a cell, tissue or organism with normal or abnormal physiology, was (re)initiated in the middle of the 1990s with the proteome project [1]. Since then, many technologies to make the analysis of the proteome a reality have been united.The perfect technological 'marriage' that made the proteome project feasible was between high resolution 2-DE for separation of large numbers of proteins and MS for identification and characterization of the proteins displayed on this gel [2]. However, before this happy union occured, science and technology devoted to protein study had a long journey until 2-DE and MS combination was possible (Fig. 1). It started in the last century, in 1937, when Tiselius [3] introduced electrophoresis technique as a modification of Reiner's early concept (1927) [4] to analyse a complex mixture of proteins such as serum proteins. Only 20 years later, in 1959, electrophoresis on a gel support formed by crosslinked polymerization of Cyanogum 41, a commercial name for two organic monomers, acrylamide and the crosslinking agent, N,N1-methylenebisacrylamide, was shown to be useful and was named PAGE by Raymond and Weintraub [5]. The adaptation of polyacrylamide gel to IEF in a stable pH gradient, developed by Svensson in 1962 [6], was possible by the end of the 1960s [7][8][9]. About this time, the 2-D by combining IEF to separate undenatured proteins according to their charge and gradient SDS-PAGE for a second separation, according to their molecular mass, was for the first time introduced by Kenrick and Margolis (1970) [10]. Only in 1975, was IEF in denaturing conditions, as known today, used to follow the 2-D PAGE and it was described in three independent works [11][12][13]. The most powerful demonstraCorrespondence: Dr. Deborah Penque, Laboratório de Proteó-mica, Departamento de Genética, Instituto Nacional de Saúde, Dr. Ricardo Jorge, I.P., A...

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“…МГ регулирует дифференцировку преадипоцитов в зрелые адипоциты. При этом в зрелых клетках этот белок практически отсутствует, а в преадипоцитах интенсивно синтезируется, но дифференцировка преадипоцитов регулируется МГ, происходящем из внешних источников [41]. Инсулин, транспортируемый МГ, усиливает экспрессию рецепторов эндоцитоза на гепатоцитах и адипоцитах, но блокирует её у макрофагов [42].…”

Section: роль ссм в регуляции нервной системыunclassified

Macroglobulin signaling system

Na¹,

Vn²

2012

BIOMED KHIM

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Обобщена и систематизирована информация о структуре сигнальной системы макроглобулинов, состоящей из самих белков семейства макроглобулинов (альфа-2-макроглобулин, ассоциированный с беременностью альфа-2-гликопротеин, ассоциированный с беременностью протеин плазмы А), их рецепторов (LRP, grp78), лигандов (протеиназы, цитокины, гормоны, липиды и пр.), трансформационных и транскрибционных факторов, влияющих на синтез данной группы белков, а также представления о свойствах белков семейства макроглобулинов, механизмах их реализации и роли белков семейства в различных физиологических и патологических процессах.Ключевые слова: макроглобулин, межклеточные взаимодействия, эндоцитоз, сигнальный рецептор, белок связанный с рецептором липопротеинов низкой плотности (LRP), трансмембранный перенос.ВВЕДЕНИЕ. Сигнальные системы позволяют осуществлять взаимодействия клеток в многоклеточных организмах путем каскада химических реакций с вовлечением широкого круга эффекторных молекул. Одной из важнейших сигнальных систем животного мира, включая человека, является сигнальная система макроглобулинов (ССМ), которая состоит из:-индукторов генов макроглобулинов; -собственно макроглобулинов; -факторов, изменяющих конформационное строение макроглобулинов; -молекул, образующих комплексы с макроглобулинами; -мембранных рецепторов макроглобулинов. СТРУКТУРА И СОСТАВ БЕЛКОВ СЕМЕЙСТВА МАКРОГЛОБУЛИНОВ.Ген основного представителя семейства макроглобулинов -a 2 -макроглобулина (МГ) имеет размер около 40 kb и состоит из 36 экзонов, кодирующих биосинтез субъединицы белка с относительной молекулярной массой около 180 кДа [1]. Субъединица МГ состоит из 12 доменов, часть из которых образуeт рецептор-связывающий домен, составляющий 42% её объёма. В a-геликальной 400 * -адресат для переписки

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Proteomic analysis of adipocyte differentiation: Evidence that α2 macroglobulin is involved in the adipose conversion of 3T3 L1 preadipocytes

Cited by 32 publications

References 40 publications

Adipose Tissue Derived Stem Cells Secretome: Soluble Factors and Their Roles in Regenerative Medicine

Adipose Tissue Derived Stem Cells Secretome: Soluble Factors and Their Roles in Regenerative Medicine

Two‐dimensional gel electrophoresis and mass spectrometry for biomarker discovery

Macroglobulin signaling system

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