Proteomic analysis of 3-MCPD and 3-MCPD dipalmitate toxicity in rat testis

Sawada, Stefanie; Oberemm, Axel; Buhrke, Thorsten; Meckert, C.; Rozycki, Christel; Braeuning, Albert; Lampen, Alfonso

doi:10.1016/j.fct.2015.06.002

Cited by 42 publications

(19 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Network analysis verified close relationships between molecular effects induced by 3‐MCPD and its dipalmitate ester. Altogether, the results indicate similar mode of action for 3‐MCPD and its esters (3‐MCPD dipalmitate) (Sawada et al., ).…”

Section: Assessmentsupporting

confidence: 58%

Risks for human health related to the presence of 3‐ and 2‐monochloropropanediol (MCPD), and their fatty acid esters, and glycidyl fatty acid esters in food

Wallace¹,

Alexander²,

Barregård³

et al. 2016

EFS2

126

View full text Add to dashboard Cite

EFSA was asked to deliver a scientific opinion on free and esterified 3-and 2-monochloropropane-1, 2-diol (MCPD) and glycidyl esters in food. Esters of 3-and 2-MCPD and glycidol are contaminants of processed vegetable oils; free MCPDs are formed in some processed foods. The Panel on Contaminants in the Food Chain (CONTAM Panel) evaluated 7,175 occurrence data. Esters of 3-and 2-MCPD and glycidyl esters were found at the highest levels in palm oil/fat, but most vegetable oil/fats contain substantial quantities. Mean middle bound (MB) dietary exposure values to total 3-MCPD, 2-MCPD and glycidol, respectively, across surveys and age groups in µg/kg body weight (bw) per day were 0.2-1.5, 0.1-0.7 and 0.1-0.9; high exposure (P95) values were 0.3-2.6, 0.2-1.2 and 0.2-2.1. Animal studies show extensive hydrolysis of esterified 3-MCPD and glycidol following oral administration; esterified and free forms were assumed to contribute equally to internal exposures. Nephrotoxicity was consistently observed in rats treated with 3-MCPD. Data on 2-MCPD toxicity were insufficient for dose-response assessments. Chronic treatment with glycidol increased the incidence of tumours in several tissues of rats and mice, likely via a genotoxic mode of action. The Panel selected a BMDL 10 value for 3-MCPD of 0.077 mg/kg bw per day for induction of renal tubular hyperplasia in rats and derived a tolerable daily intake (TDI) of 0.8 µg/kg bw per day. The mean exposure to 3-MCPD was above the TDI for 'Infants', 'Toddlers' and 'Other children'. For glycidol, the Panel selected a T25 value of 10.2 mg/kg bw per day for neoplastic effects in rats. The margins of exposure (MoEs) were 11,300-102,000 and 4,900-51,000 across surveys and age groups at mean and P95 exposures, respectively. An exposure scenario for infants receiving formula only resulted in MoEs of 5,500 (mean) and 2,100 (P95). MoEs of 25,000 or higher were considered of low health concern. Amendment: A few editorial amendments were carried out that do not materially affect the contents of the outcome of this scientific output: on pages 5 and 91 'chorine' has been replaced with 'chlorine'; on page 52 the word 'it' has been replaced by 'its' in the first paragraph after Figure 7; on page 54 in the Repeated dose section the reference to MCPD has been replaced with 3-MCPD; on page 54 the 429.5 mg/kg bw day dose has been replaced with 29.5 mg/kg bw day; on page 55 in the first paragraph of the Hematology section the reference 'mg/kg per day' has been replaced with 'mg/kg bw per day; on page 55 the year for the reference Marchesini et al. has been changed from 1989 to 1986; on page 59, in the first paragraph of the Developmental toxicity section the word 'days' has been cancelled; on page 65 the reference 'Onami et al., 2014a,b' has been replaced with 'Onami et al., 2014b'; on page 85 first paragraph, reference to the lowest T25 of 10 has been replaced to 10.2. To avoid confusion, the older version has been removed from the EFSA Journal, but is available on request, as is a version sho...

show abstract

Section: Assessmentsupporting

confidence: 58%

Risks for human health related to the presence of 3‐ and 2‐monochloropropanediol (MCPD), and their fatty acid esters, and glycidyl fatty acid esters in food

Wallace¹,

Alexander²,

Barregård³

et al. 2016

EFS2

126

View full text Add to dashboard Cite

show abstract

“…Controls were incubated with 0.1% (v/v) EtOH as a solvent control. For harvesting, cells were washed three times with pre-chilled phosphate-buffered saline and subsequently lysed with lysis buffer (7 M urea, 2 M thiourea, 2% pharmalyte (pH3-10), 0.7% spermine, 1.2% destreak reagent, 4% chaps, serdolit mb-1, and proteinase inhibitor) [3] under shaking for 30 min following centrifugation at 100,000 g for 60 min at 15 °C. Supernatant was stored at −80 °C for further analysis.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…Data analysis and statistical evaluation of 2D gels using the software R has been described in detail in a previous paper [3] . For details on the experimental setup, please refer to the latter publication.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…Mass spectrometric analyses were used for protein identification, whereas protein quantification was based on spot intensity evaluation of 2D gel electrophoresis (see also section 2.2). Protein identification by mass spectrometry has been described in detail in a previous paper [3] . For details on the experimental setup, please refer to the latter publication.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Comparative proteomic data of M13SV1 human breast epithelial cells and their tumorigenic variants under treatment with estrogenic compounds

et al. 2016

Self Cite

View full text Add to dashboard Cite

Data from a comparative proteomic analysis of three human breast epithelial cell lines are presented. M13SV1 cells and their tumorigenic derivatives M13SV1-R2-2 and M13SV1-R2-N1 were used. Proteomic data were obtained using 2-dimensional gel electrophoresis and subsequent identification of proteins by MALDI-TOF mass spectrometry. In a second experiment, the three cell lines were treated with different concentrations of the estrogenic compounds β-estradiol or genistein and alterations in protein expression were monitored by 2-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. Presented data provide a comprehensive overview of proteomic differences between the three cell lines and their response to estrogenic stimulation.

show abstract

“…3-MCPD has been associated to reproductive impact in adult rats (Sun et al, 2013, Sawada et al, 2015, but the effects of the exposure from the prepubertal period are unknown. Spermatogenesis and steroidogenesis are not yet fully established during prepuberty, which in rat occurs at postnatal day 36-55 or 60 (Clegg, 1960, Ojeda, Andrews, Advis, & White, 1980.…”

Section: Introductionmentioning

confidence: 99%

<b>Experimental exposure to 3-monochloropropane-1,2-diol from the pre-puberty causes damage in sperm production and motility in adulthood

Vieira¹,

Favareto²

2017

Acta Sci. Biol. Sci.

View full text Add to dashboard Cite

Monochloropropane-1,2-diol (3-MCPD) is a food contaminant that can be formed during the thermic processing of various foodstuffs. Studies of reproductive toxicology of 3-MCPD are mainly concentrated in the evaluation of possible insults caused by exposure of adult animals. However, the prepuberty might be a period of different susceptibility to chemicals. The aim of this study was to evaluate the effects on reproductive endpoints of the 3-MCPD-exposure prepubertal male rats. Wistar male rats were assigned to 4 groups: control and exposed to 2.5; 5 or 10 mg kg-1 day-1 of 3-MCPD for 30 days by gavage. Testis and epididymis were used for sperm counts and histology analysis. Sertoli cell number and dynamic of the spermatogenesis were evaluated. Sperm were collected from the vas deferens for evaluation of the sperm motility and morphology. Number of sperm with progressive movement, number of Sertoli cells and germ cells and relative daily sperm production were decreased in the groups exposed to 5 and 10 mg kg-1 day-1 of 3-MCPD. Sperm morphology, testicular and epididymal histology were comparable among groups. Results show that 3-MCPD-exposure of rats from prepuberty might cause alterations in spermatogenesis and sperm maturation, similarly to exposure in adulthood.

show abstract

Proteomic analysis of 3-MCPD and 3-MCPD dipalmitate toxicity in rat testis

Cited by 42 publications

References 39 publications

Risks for human health related to the presence of 3‐ and 2‐monochloropropanediol (MCPD), and their fatty acid esters, and glycidyl fatty acid esters in food

Risks for human health related to the presence of 3‐ and 2‐monochloropropanediol (MCPD), and their fatty acid esters, and glycidyl fatty acid esters in food

Comparative proteomic data of M13SV1 human breast epithelial cells and their tumorigenic variants under treatment with estrogenic compounds

<b>Experimental exposure to 3-monochloropropane-1,2-diol from the pre-puberty causes damage in sperm production and motility in adulthood

Contact Info

Product

Resources

About