Proteomic analysis in the model organism Daphnia has the potential to unravel molecular pathways involved in phenotypic changes in response to changing environmental conditions

Otte, Kathrin A.; Effertz, Christoph; Fröhlich, Thomas; Arnold, Georg J.; Laforsch, Christian; Elert, Eric von

doi:10.1007/s10750-018-3718-5

Hydrobiologia

2018

DOI: 10.1007/s10750-018-3718-5

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Proteomic analysis in the model organism Daphnia has the potential to unravel molecular pathways involved in phenotypic changes in response to changing environmental conditions

Kathrin A. Otte

Christoph Effertz

Thomas Fröhlich

et al.

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Cited by 3 publications

(2 citation statements)

References 70 publications

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“…Although the overall number of proteomics studies on Daphnia is still limited, most of them focus on the effects of various toxins [8][9][10][11]. When investigating Daphnia in ecotoxicoproteomic studies, besides interfering gut proteases [12], a common drawback are highly abundant proteins like egg-yolk vitellogenin, preventing the identification of lower abundant proteins. To increase the analytical depth of LC-MS/MS-based proteome analysis, we tested a procedure based on the separate analysis of whole daphnids and dissected daphnid guts combined with a data-independent acquisition (DIA) strategy [13].…”

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confidence: 99%

Improving the proteome coverage of Daphnia magna ‐ implications for future ecotoxicoproteomics studies

Wilde¹,

Brehm

Schwarzer

et al. 2022

Proteomics

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Aquatic pollution is an increasing problem and requires extensive research efforts to understand associated consequences and to find suitable solutions. The crustacean Daphnia is a keystone species in lacustrine ecosystems by connecting primary producers with higher trophic levels. Therefore, Daphnia is perfectly suitable to investigate biological effects of freshwater pollution and is frequently used as an important model organism in ecotoxicology. The field of ecotoxicoproteomics has become increasingly prevalent, as proteins are important for an organism's physiology and respond rapidly to changing environmental conditions. However, one obstacle in proteome analysis of Daphnia is highly abundant proteins like vitellogenin, decreasing the analytical depth of proteome analysis. To improve proteome coverage in Daphnia, we established an easy‐to‐use procedure based on the LC‐MS/MS of whole daphnids and the dissected Daphnia gut, which is the main tissue getting in contact with soluble and particulate pollutants, separately. Using a comprehensive spectral library, generated by gas‐phase fractionation and a data‐independent acquisition method, we identified 4621 and 5233 protein groups at high confidence (false discovery rate < 0.01) in Daphnia and Daphnia gut samples, respectively. By combining both datasets, a proteome coverage of 6027 proteins was achieved, demonstrating the effectiveness of our approach.

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confidence: 99%

Improving the proteome coverage of Daphnia magna ‐ implications for future ecotoxicoproteomics studies

Wilde¹,

Brehm

Schwarzer

et al. 2022

Proteomics

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“…In fact, it is possible to link candidate genome regions to selection pressures resulting from, e.g., stressors such as predation, parasitism, or toxins released during cyanobacteria blooms. Genomic approaches are now also rounded up by proteomic analysis in order to obtain a more comprehensive understanding of the associated molecular pathways (Otte et al, 2018).…”

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Preface: Blasts from the past and back to the future

2019

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Following the kind invitation of the organizers Christian Laforsch and Max Rabus, the 11th International Symposium on Cladocera gathered scientists from all over the world working on multidisciplinary aspects of Cladoceran biology. The meeting is renowned to house Cladoceran research from multiple disciplines including evolutionary history, host-parasite and predator-prey interactions, phylogeny, environmental genomics, population genetics, biogeography, systematics, and taxonomy. First being held in 1986 in Hungary, this meeting recurs every 3 years. The 11th meeting was held in Kulmbach, Germany, from September the 24th to the 29th, 2017, in the wonderful Plassenburg Castle in the margravial city of Kulmbach, Germany. During this 1 week, scientists from all around the globe coming from Austria,

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Combined effects of the pesticide spinetoram and the cyanobacterium Microcystis on the water flea Daphnia pulex

Shen

Zhan

Jia

et al. 2022

Environ Sci Pollut Res

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Spinetoram is one of the most worldwidely used pesticides for its high insecticidal e cacy and low human toxicity Following the large usage of spinetoram, the ecotoxicity and environmental risks to aquatic ecosystems have call for urgent study In the present study, we investigated the combined effects of spinetoram and the harmful alga Microcystis aeruginosa in freshwater, on survival and reproduction of Daphnia pulex Acute toxicity test of spinetoram resulted in negative effects on survival, with a 48h-LC 50 value of 3771 µg L -1 Under the long-time exposure to environmentally relevant concentrations (018 and 035 µg L -1 ) of spinetoram and a low composition of Microcystis (30%) in the diet, D pulex showed both shorter longevity and lower fecundity, the time to rst brood was also increased At population level, carrying capacity was highly decreased by spinetoram and Microcystis, whereas a signi cant decrease of intrinsic growth rate was observed at 035 µg L -1 spinetoram with 30% Microcystis as food The present study highlighted that pesticide spinetoram had highly toxic effects on D pulex and could reduce the tolerance of D pulex to M aeruginosa, causing great effects on D pulex population in natural waterbodies Authors information

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Proteomic analysis in the model organism Daphnia has the potential to unravel molecular pathways involved in phenotypic changes in response to changing environmental conditions

Cited by 3 publications

References 70 publications

Improving the proteome coverage of Daphnia magna ‐ implications for future ecotoxicoproteomics studies

Improving the proteome coverage of Daphnia magna ‐ implications for future ecotoxicoproteomics studies

Preface: Blasts from the past and back to the future

Combined effects of the pesticide spinetoram and the cyanobacterium Microcystis on the water flea Daphnia pulex

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