Proteomic analysis identifies highly expressed plasma membrane proteins for detection and therapeutic targeting of specific breast cancer subtypes

Abstract

In recent years, there has been an emphasis on personalizing breast cancer treatment in order to avoid the debilitating side effects caused by broad-spectrum chemotherapeutic drug treatment. Development of personalized medicine requires the identification of proteins that are expressed by individual tumors. Herein, we reveal the identity of plasma membrane proteins that are overexpressed in estrogen receptor α-positive, HER2-positive, and triple negative breast cancer cells. The proteins we identified are involved in maintaining protein structure, intracellular homeostasis, and cellular architecture; enhancing cell proliferation and invasion; and influencing cell migration. These proteins may be useful for breast cancer detection and/or treatment.Electronic supplementary materialThe online version of this article (10.1186/s12014-018-9206-0) contains supplementary material, which is available to authorized users.

Antitumor impact of iron oxide nanoparticles in Ehrlich carcinoma-bearing mice

“…Figure 3 showed the average FTIR spectra of control, tumor, and tumor + iron nanoparticles tissue samples. Notably, the tumor sample demonstrated different spectra compare to both control and tumor + iron nanoparticles samples, the amide II (1540-1570 cm −1 ) and amide I (1600-1700 cm −1 ) bands have the uppermost absorption peaks, which was confirmed by data in (Ziegler et al, 2018(Ziegler et al, , 2014. Surprisingly, the three samples were separated at the absorption peaks around 2925 cm −1 and 2850 cm −1 .…”

Metal Organic Framework Nanomaterial-Based Extraction and Proteome Analysis of Membrane and Membrane-Associated Proteins

“…7,8 The first strategy is based on the difference in physicochemical properties, which includes ultracentrifugation, density gradient centrifugation, 9 and twophase partitioning. 10 The second strategy captures MPs through interaction with lectins 11 or antibodies, and the third strategy achieves enrichment by chemical covalent coupling with surface groups of MPs, for instance, cell-surface biotinylation. 12 Although the extraction and identification of MPs have been improved with the development of various methods, difficulties still exist because of their own limitations.…”

On chip complex breast tumour microenvironment: application to research in nanomedicine

“…Additionally, the confinement allows for a controlled continuous perfusion of media, limiting the need of daily refreshment [93]. Moreover the perfusion can be optimized to reproduce shear stress levels found in in vivo tissues (e.g., blood vessel shear stress) [94], on the cells present in the microfluidic device. Indeed, cells exposed to certain stresses have shown to change their morphology and behaviour [95,96].…”

“…A literature analysis, performed on the 24 proteins presenting significant variations between the different spheroid sizes, identified 16 proteins labeled as key components in the regulation of different cancers (e.g., colon, lung, prostate, pancreas). Additionally, 5 of these proteins were found to be over-expressed in human 2D in vitro (Nudt5, Tpp2) or in vivo (EpCAM, Fabp5, Nqo1) breast cancer studies [91][92][93][94][95]. Furthermore, Nqo1 is considered as a good biomarker for the prognostic of breast cancer [93].…”

“…Finally, Tripeptidylpeptidase 2 (Tpp2) is an enzyme involved in a wide variety of molecular functions, including DNA repair and cell apoptosis. Tpp2 has been reported to participate in the mitotic exit of malignant cells, promoting the cell division and therefore tumour proliferation [94]. Altogether, these four up-regulated proteins have shown involvement in cancer (e.g., thyroid, heart, kidney), but further investigation is required to assess their involvement in breast cancer.…”