Proteomic analysis identified potential age‐associated prognostic biomarkers in pneumonia‐derived paediatric sepsis

Luo, Ting; Yan, Huan; Li, Xun; Deng, Youcai; Huang, Jiaotian; Li, Liping; Xiao, Zhenghui; Lu, Xiulan

doi:10.1002/prca.202100036

Cited by 7 publications

(6 citation statements)

References 32 publications

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“…The protein-protein interaction analysis of the differentially expressed proteins between the sepsis and healthy groups identi ed the top ve nodes with the highest degrees as ALB (36), AHSG (28), APOA1 (28), APOB (27), and A2M (25) (see Fig. 2E).…”

Section: Protein-protein Interaction Network Of Differentially Expres...mentioning

confidence: 99%

Peptidomics Characteristics of Pediatric Sepsis

Yan,

Li,

Luo

et al. 2024

Preprint

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This study explores the significant differential expression of peptides in sepsis patients compared to healthy controls and those with common infections using plasma peptidomics analysis. Blood samples were collected from 10 pediatric sepsis patients admitted to Hunan Children's Hospital in 2021, along with 20 age- and sex-matched healthy controls and 5 children with common infections. Differential peptide precursor proteins underwent GO and KEGG pathway enrichment analyses and protein-protein interaction analysis using the STRING database. A total of 3149 endogenous peptides corresponding to 480 precursor proteins were identified. Compared to the healthy group, the sepsis group exhibited 1113 differential peptides, with 880 upregulated and 233 downregulated. Compared to the common infection group, the sepsis group showed 181 upregulated and 86 downregulated peptides. These differences were primarily in humoral immune response and complement and coagulation cascades. The peptide RSFFSFLGEA, associated with the precursor protein SAA1, had the highest LogFC values between sepsis and healthy groups, and sepsis and common infection groups, with values of 6.828 and 5.580, respectively. This study reveals specific changes in peptide expression in sepsis patients' plasma, particularly significant alterations in peptides related to SAA1, complement C3, HB, and HP. These peptides are involved in acute inflammatory response, complement system, and free hemoglobin pathways, indicating their crucial roles in sepsis pathology. These findings provide new insights into the mechanisms of sepsis and suggest potential applications of these peptides in sepsis diagnosis and treatment, aiming to improve early diagnosis and therapeutic outcomes.

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Section: Protein-protein Interaction Network Of Differentially Expres...mentioning

confidence: 99%

Peptidomics Characteristics of Pediatric Sepsis

Yan,

Li,

Luo

et al. 2024

Preprint

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“…Due to the high cost of experimental tests, only a few researchers used proteomics for pediatric sepsis biomarker screening. Luo et al 79 have performed a quantitative identification of plasma proteome to analyze the age-related proteomic profile of sepsis in children. They found that developmental age may affect the levels and biological functions of haptoglobin and thrombin-reactive protein 1 (THBS1).…”

Section: Biomarkers Screened By Proteomicsmentioning

confidence: 99%

“…Due to the high cost of experimental tests, only a few researchers used proteomics for pediatric sepsis biomarker screening. Luo et al 79 . have performed a quantitative identification of plasma proteome to analyze the age‐related proteomic profile of sepsis in children.…”

Section: Biomarkers Screened By Proteomicsmentioning

confidence: 99%

Multilevel omics for the discovery of biomarkers in pediatric sepsis

Wang,

Li,

Qian

et al. 2023

Pediatric Investigation

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Severe sepsis causes organ dysfunction and continues to be the leading reason for pediatric death worldwide. Early recognition of sepsis could substantially promote precision treatment and reduce the risk of pediatric death. The host cellular response to infection during sepsis between adults and pediatrics could be significantly different. A growing body of studies focused on finding markers in pediatric sepsis in recent years using multi‐omics approaches. This narrative review summarized the progress in studying pediatric sepsis biomarkers from genome, transcript, protein, and metabolite levels according to the omics technique that has been applied for biomarker screening. It is most likely not a single biomarker could work for precision diagnosis of sepsis, but a panel of markers and probably a combination of markers detected at multi‐levels. Importantly, we emphasize the importance of group distinction of infectious agents in sepsis patients for biomarker identification, because the host response to infection of bacteria, virus, or fungus could be substantially different and thus the results of biomarker screening. Further studies on the investigation of sepsis biomarkers that were caused by a specific group of infectious agents should be encouraged in the future, which will better improve the clinical execution of personalized medicine for pediatric sepsis.

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“… 8 Others suggested that cholesterol metabolism and the elevated levels of several apolipoproteins may have a protective effect on the disease. 9 , 10 So, a firmed conclusion has not been drawn until then.…”

Section: Introductionmentioning

confidence: 99%