Proteomic analysis demonstrates the role of the quality control protease LONP1 in mitochondrial protein aggregation

Abstract

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Increased Systemic Antioxidant Power Ameliorates the Aging-Related Reduction in Oocyte Competence in Mice

“…The similar levels of PRKN activation in young and açaí-treated aged oocytes suggest that the mitochondrial quality was improved by açaí such that mitophagy was not triggered. The improved oocyte quality was likely not due to mitochondrial protein quality control, as the activation of the mitochondrial UPR—as determined by LonP1 and HSP60 protein levels—was blunted in aged oocytes, as previously observed [ 44 ], and this was not affected by antioxidants.…”

Organismal and Cellular Stress Responses upon Disruption of Mitochondrial Lonp1 Protease

“…However, siRNA-induced LonP1 disruption did not induce any of the above stress response pathways in WM266-4 metastatic melanoma cells, advocating in favor of the presence of additional compensatory mechanisms, owing to specific cellular demands. Similarly, a stable genetic LONP1 knockdown (gKD) in HeLa cells, with a significant reduction in cellular protein levels, did not cause severe alterations in mitochondrial structure and function or strong defects in mitochondrial protein degradation efficiency, at least under normal conditions [99].…”

Fumonisin B2 Induces Mitochondrial Stress and Mitophagy in Human Embryonic Kidney (Hek293) Cells—A Preliminary Study

“…LONP1 reduces the effects of stress via the degradation of oxidatively damaged and misfolded proteins [ 20 , 44 ]. SIRT3 post-translationally regulates LONP1 via deacetylation.…”

“…FB 2 did not alter LONP1 expression despite the downregulation of SIRT3 ( Figure 4 C) and upregulation of ROS ( Figure 2 A). LONP1 (an ATP-dependent protease) contains a highly conserved ATPase domain with an AAA + module and a proteolytic domain with an N-terminal domain [ 20 , 44 ]. FB 2 depleted cellular ATP levels and consequently may have inhibited LONP1 catalytic activity and the degradation of oxidised proteins ( Figure 3 A).…”