Proteomic Analysis and Identification of Possible Allergenic Proteins in Mature Pollen of Populus tomentosa

Rui, Wang; Zhang, Jin; Fan, Wei; Lu, Mengzhu; Hu, Jianjun

doi:10.3390/ijms19010250

Cited by 12 publications

(10 citation statements)

References 47 publications

(61 reference statements)

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“…In this study, three spots (spots 58–60) were attributed to proteins with no clearly predicted, well-defined function. This is consistent with the findings of other pollen proteomic studies [14,46].…”

Section: Discussionsupporting

confidence: 93%

Cytological and Proteomic Analysis of Wheat Pollen Abortion Induced by Chemical Hybridization Agent

Wang

Zhang

Fang

et al. 2019

IJMS

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In plants, pollen grain transfers the haploid male genetic material from anther to stigma, both between flowers (cross-pollination) and within the same flower (self-pollination). In order to better understand chemical hybridizing agent (CHA) SQ-1-induced pollen abortion in wheat, comparative cytological and proteomic analyses were conducted. Results indicated that pollen grains underwent serious structural injury, including cell division abnormality, nutritional deficiencies, pollen wall defect and pollen grain malformations in the CHA-SQ-1-treated plants, resulting in pollen abortion and male sterility. A total of 61 proteins showed statistically significant differences in abundance, among which 18 proteins were highly abundant and 43 proteins were less abundant in CHA-SQ-1 treated plants. 60 proteins were successfully identified using MALDI-TOF/TOF mass spectrometry. These proteins were found to be involved in pollen maturation and showed a change in the abundance of a battery of proteins involved in multiple biological processes, including pollen development, carbohydrate and energy metabolism, stress response, protein metabolism. Interactions between these proteins were predicted using bioinformatics analysis. Gene ontology and pathway analyses revealed that the majority of the identified proteins were involved in carbohydrate and energy metabolism. Accordingly, a protein-protein interaction network involving in pollen abortion was proposed. These results provide information for the molecular events underlying CHA-SQ-1-induced pollen abortion and may serve as an additional guide for practical hybrid breeding.

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Section: Discussionsupporting

confidence: 93%

Cytological and Proteomic Analysis of Wheat Pollen Abortion Induced by Chemical Hybridization Agent

Wang

Zhang

Fang

et al. 2019

IJMS

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“…Label-free quantification proteomics was applied in the study, as there is no required labeling reagents and limit to samples in experiments [32]. Compared to most proteomics research in Populus [16][17][18], more proteins were identified in our study (Figure 1a). It could be due to the main advantages of label-free quantification, which can achieve higher proteome coverage as compared to the most widely used 2-DE in Populus proteome [1,[16][17][18].…”

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

“…Liu et al presented a proteome profiling of Populus early stem from primary to secondary growth using two-dimensional gel electrophoresis (2-DE) followed by LC-ESI-Q-TOF-MS, identifying 165 differential abundant proteins during various growth stages of Populus early stems [15]. Wang et al analyzed the mature Populus pollen proteome, identifying 159 distinct proteins from 242 spots analyzed by 2-DE and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/TOF MS) [16]. Leijon et al used a similar approach to identify 201 proteins specifically enriched in responses to stress, transport, metabolism and signal transduction from plasmodesmata of Populus cell suspension cultures [17].…”

Section: Introductionmentioning

confidence: 99%

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Comparative Proteomic Analysis of Leaves at Different Ages in Allotriploid Populus

Wang

Wei

et al. 2020

Forests

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Triploid poplar trees have been shown to have a number of growth advantages, especially much bigger leaves that contribute greatly to the increased biomass. In this study, we focused on the relationships between leaf age and leaf metabolism in triploids. We performed comparative proteomic analysis of the 5th (FDR5), 10th (FDR10), and 25th (FDR25) leaves from the apical meristems in allotriploids originated from first-division restitution (FDR). A total of 1970, 1916, and 1850 proteins were identified in the FDR5, FDR10, and FDR25, respectively. Principle component analysis (PCA) and differentially accumulated protein (DAP) analysis showed that FDR10 and FDR25 displayed higher similarities of protein accumulation patterns as compared to FDR5. MapMan enrichment analysis showed that several primary metabolic pathways or processes were significantly enriched in the DAPs. For example, photosynthesis, major CHO metabolism, glycolysis, N metabolism, redox, C1-metabolism, DNA, and protein turnover were significantly altered in both FDR10 and FDR25 compared with FDR5. In addition, amino acid metabolism and gluconeogenesis/glyoxylate cycle also underwent significant changes in FDR25 compared with FDR5. However, only amino acid metabolism was significantly enriched in the DAPs between FDR25 and FDR10. Further, DAP accumulation pattern analysis implied that FDR5, FDR10, and FDR25 were placed in the young, mature, and primary senescence stages of leaves. The most DAPs involved in the light reaction, photorespiration, Calvin cycle, starch and sucrose metabolism, pentose phosphate pathway (OPP), tricarboxylic acid (TCA) cycle, N metabolism, and C1-metabolism displayed higher accumulation in both FDR10 and FDR25 compared to FDR5. However, the most DAPs that are involved in cell wall and lipid metabolism, tetrapyrrole synthesis, nucleotide metabolism exhibited lower accumulation in both FDR10 and FDR25. Almost all DAPs between FDR-10 and FDR-25 showed a dramatic decrease in FDR25. KEGG enrichment analysis showed that carbon metabolism was altered significantly at different leaf ages. DAPs that are involved in carbon metabolism were predicted as different points in protein–protein interaction (PPI) networks from the STRING database. Finally, inconsistent transcript and protein abundance was found for DAPs, indicating the presence of posttranscriptional regulation during leaf-age progression process.

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“…Proteomics analysis was used to characterize the allergens from P. tomentosa mature pollen. In total, 27 proteins were identified as putative allergens, such as heat shock protein, enolase, pollen Ole e 1 allergen, thioredoxin and profilins using the Structural Database of Allergenic Proteins (SDAP) tool and Allergen Online (9).…”

Section: Populus Familymentioning

confidence: 99%

Respiratory allergies: Salicaceae sensitization (Review)

et al. 2021

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Seasonal allergic rhinitis (SAR) is one of the most frequent chronic conditions of the modern world. Pollen carried by the wind from pollinated trees is a major source of SAR. Betulaceae, Oleaceae and Platanus are the most important sources of airway sensitization with regard to tree pollen and, therefore, they are included in the official recommendations of skin prick testing by different official societies. Salicaceae pollen is a moderate source of pollen sensitization. Conversely, large areas are covered with poplars and willows around the world. A number of studies from many countries showed that in some particular situations (large and compacted areas covered by Salicaceae, weather conditions, air pollution, urban ornamental vegetation), poplar and willow pollens may become of local importance in producing SAR. The aim of this review was to present a synthesis of information regarding Salicaceae pollen allergy showing that, if various unfavorable aspects are brought together, a minor problem (Salicaceae sensitization) can became a public health problem. Contents 1. Introduction 2. Populus family 3. Salix family 4. Conclusions

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Proteomic Analysis and Identification of Possible Allergenic Proteins in Mature Pollen of Populus tomentosa

Cited by 12 publications

References 47 publications

Cytological and Proteomic Analysis of Wheat Pollen Abortion Induced by Chemical Hybridization Agent

Cytological and Proteomic Analysis of Wheat Pollen Abortion Induced by Chemical Hybridization Agent

Comparative Proteomic Analysis of Leaves at Different Ages in Allotriploid Populus

Respiratory allergies: Salicaceae sensitization (Review)

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