Proteomic analyses of decellularized porcine ovaries identified new matrisome proteins and spatial differences across and within ovarian compartments

Henning, N; LeDuc, Richard D.; Even, Kelly A.; Laronda, Monica M.

doi:10.1038/s41598-019-56454-3

Cited by 44 publications

(37 citation statements)

References 38 publications

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“…Therefore, the protection from the radiation injuries is of great practical significance in protecting the human body, especially for the highly metabolized and rapidly renewing germ cells. Radiation might lead to POI or even POF, the final phase of POI, which is a female endocrine disease, with the FSH ≥40 IU/L [ 18 ]. The main clinical manifestations of infertility (due to high gonadotropin and low estrogen) may cause the long-term menopausal symptoms, inducing harm to the women's physical and mental health.…”

Section: Discussionmentioning

confidence: 99%

Premature Ovarian Insufficiency (POI) Induced by Dynamic Intensity Modulated Radiation Therapy via P13K-AKT-FOXO3a in Rat Models

Long

et al. 2021

BioMed Research International

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This study is aimed to investigate the mechanisms of radiation-induced mouse models of premature ovarian insufficiency (POI). Wistar female rats were grouped into the control, 3.2 Gy, 4.0 Gy, and 4.8 Gy groups. Overall ovarian functions were assessed with the H&E staining and ELISA. Proinflammatory cytokine secretion was analyzed ELISA, and the reactive oxygen species (ROS) levels were analyzed with immunohistochemistry. Protein expressions were analyzed by Western blot analysis. The 4.0 Gy and 4.8 Gy groups had significantly lower ovarian weight coefficients than the control and 3.2 Gy groups (after only one irradiation therapy). The 3.2 Gy radiation group induced periodic disturbance and hormone change at 4 weeks after radiation. In the 4.0 Gy and 4.8 Gy groups, the preantral follicles and antral follicles were decreased, while Atresia follicles were increased. E2 was decreased, while FSH and LH secretions were increased. The ovaries in the 4.0 Gy group were not completely atrophied, and some preantral follicles remained. Ovarian atrophy and follicular Atresia were found in the 4.8 Gy group. Inflammatory and oxidative markers were upregulated. PI3K and AKT were downregulated in the 4.0 Gy and 4.8 Gy groups, while FOXO3a was upregulated. Ovarian injuries may lead to oxidative damages and inflammatory injuries, downregulate the expression of P13k and Akt, upregulate the expression of FOXO3a, and lead to follicular atresia in the ovary.

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Section: Discussionmentioning

confidence: 99%

Premature Ovarian Insufficiency (POI) Induced by Dynamic Intensity Modulated Radiation Therapy via P13K-AKT-FOXO3a in Rat Models

Long

et al. 2021

BioMed Research International

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“…There is also an opportunity to combine SW ultrasound elastography with other complementary imaging techniques, including second harmonic generation, as has been done with prostate biopsies, 66 to gain new insights into how the structure and organization of ECM proteins contribute to the mechanical properties of tissues. Finally, SW ultrasound elastography data could be used to build three-dimensional (3D) maps of SW velocity and compared to 3D maps of ECM proteins 67 to interrogate how matrix composition influences SW propagation.…”

Section: Discussionmentioning

confidence: 99%

Ultrasound Shear Wave Velocity Varies Across Anatomical Region in Ex Vivo Bovine Ovaries

Gargus

Jakubowski

Arenas

et al. 2020

Tissue Engineering Part A

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The physical properties of the ovarian extracellular matrix (ECM) regulate the function of ovarian cells, specifically the ability of the ovary to maintain a quiescent primordial follicle pool while allowing a subset of follicles to grow and mature in the estrous cycle. Design of a long-term, cycling artificial ovary has been hindered by the limited information regarding the mechanical properties of the ovary. In particular, differences in the mechanical properties of the two ovarian compartments, the cortex and medulla, have never been quantified. Shear wave (SW) ultrasound elastography is an imaging modality that enables assessment of material properties, such as the mechanical properties, based on the velocity of SWs, and visualization of internal anatomy, when coupled with B-mode ultrasound. We used SW ultrasound elastography to assess whole, ex vivo bovine ovaries. We demonstrated, for the first time, a difference in mechanical properties, as inferred from SW velocity, between the cortex and medulla, as measured along the length (cortex: 2.57-0.53 m/s, medulla: 2.87-0.77 m/s, p < 0.0001) and width (cortex: 2.99-0.81 m/s, medulla: 3.24-0.97 m/s, p < 0.05) and that the spatial distribution and magnitude of SW velocity vary between these two anatomical planes. This work contributes to a larger body of literature assessing the mechanical properties of the ovary and related cells and specialized ECMs and will enable the rational design of biomimetic tissue engineered models and durable bioprostheses. Impact Statement Shear wave (SW) ultrasound elastography can be used to simultaneously assess the material properties and tissue structures when accompanied with B-mode ultrasound. We report a quantitative difference in mechanical properties, as inferred from SW velocity, between the cortex and medulla, with SW velocity being 11.4% and 8.4% higher in the medulla than the cortex when measured along the length and width, respectively. This investigation into the spatial and temporal variation in SW velocity in bovine ovaries will encourage and improve design of more biomimetic scaffolds for ovarian tissue engineering.

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“…56 Omics technologies such as proteomics or singlecell transcriptomics address issues with regards to characterisation of cells and matrices. [139][140][141][142] Novel non-destructive imaging techniques, such as micro-CT, optimal coherence tomography or 2photon microscopy promise to overcome the barrier of increased tissue thickness. [143][144][145] The use of second-harmonic generation or modified amino acids, such as azide-labelled proline, provides an ideal system for visualising matrix modifications.…”

Section: Challenges and Future Directionsmentioning

confidence: 99%

Tissue engineering of the biliary tract and modelling of cholestatic disorders

Brevini

Tysoe

Sampaziotis

2020

Journal of Hepatology

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Our insight into the pathogenesis of cholestatic liver disease remains limited, partly owing to challenges in capturing the multitude of factors that contribute to disease pathogenesis in vitro. Tissue engineering could address this challenge by combining cells, materials and fabrication strategies into dynamic modelling platforms, recapitulating the multifaceted aetiology of cholangiopathies. Herein, we review the advantages and limitations of platforms for bioengineering the biliary tree, looking at how these can be applied to model biliary disorders, as well as exploring future directions for the field.

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Proteomic analyses of decellularized porcine ovaries identified new matrisome proteins and spatial differences across and within ovarian compartments

Cited by 44 publications

References 38 publications

Premature Ovarian Insufficiency (POI) Induced by Dynamic Intensity Modulated Radiation Therapy via P13K-AKT-FOXO3a in Rat Models

Premature Ovarian Insufficiency (POI) Induced by Dynamic Intensity Modulated Radiation Therapy via P13K-AKT-FOXO3a in Rat Models

Ultrasound Shear Wave Velocity Varies Across Anatomical Region in Ex Vivo Bovine Ovaries

Tissue engineering of the biliary tract and modelling of cholestatic disorders

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