Proteomic adaptation to chronic high intensity swimming training in the rat heart

Sun, Bo; Wang, Jun hong; Lv, Yuan Yuan; Zhu, Shu; Yang, Jin; Zheng, Ji

doi:10.1016/j.cbd.2007.11.001

Cited by 26 publications

(28 citation statements)

References 36 publications

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“…Research found significantly lower protein levels of IDH2 (93%) and depressed total IDH activities (68%) in SHR heart mitochondria. IDH1 appeared to have a higher level in SD rats undergoing 8 weeks of swimming training (36). IDH activity increased 32% in the 6 weeks of treadmill running in trained intermyofibrillar mitochondria (37).…”

Section: Discussionmentioning

confidence: 91%

Aortic wall proteomic analysis in spontaneously hypertensive rats with a blood pressure decrease induced by 6-week load-free swimming

Hong

Li²,

Zhang

et al. 2015

Biomedical Reports

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Abstract. Decreased arterial compliance is one of the earliest detectable manifestations of adverse structural and functional changes within the vessel wall in hypertension. The proteomic approach is a powerful technique to analyze a complex mixture of proteins in various settings. Physical activity level was negatively associated with blood pressure. Sixteen 4-week-old male spontaneously hypertensive rats (SHR) and 16 Wistar-Kyoto (WKY) rats were randomly divided into four groups: i) SHR exercise group, ii) SHR rest group, iii) WKY exercise group and iv) WKY rest group. In the SHR and WKY exercise groups, rats were treated with a 6-week load-free swimming protocol (1 h/day, 5 days/week). The blood pressure of the rats was tested by the CODA TM2 single non-invasive blood pressure measurement appliance. After the 6-week swimming protocol, the total aorta excluding abdominal aorta was extracted. The proteins were separated by two-dimensional gel electrophoresis and identified via LC-mass spectrometry (MS)/MS. After 6-week load-free swimming, blood pressure decreased in the SHRs. Compared with sedentary SHRs, 11 spots on the 2D-gel showed a significant difference in exercised SHRs. Nine of these were chosen for further identification. There were 5 upregulated proteins (long-chain specific acyl-CoA dehydrogenase, heat shock protein β-1, isocitrate dehydrogenase subunit α, actin, α cardiac muscle 1 preprotein and calmodulin isoform 2) and 4 downregulated proteins (adipocyte-type fatty acid-binding protein, tubulin β-2C chain, 78 kDa glucose-regulated protein precursor and mimecan). Proteomics is an effective method to identify the target proteins of exercise intervention for hypertension. IntroductionHypertension is the most frequent chronic disease in the developed world. It is a multisystemic disease that affects the heart and kidneys among other organs. The overall functional, structural and biochemical alterations in vasculature have been extensively studied during hypertension, but the molecular mechanisms remain unclear. Decreased arterial compliance is one of the earliest detectable manifestations of adverse structural and functional changes within the vessel wall (1). It has been shown that the proteomic approach is a useful technique to analyze a complex mixture of proteins in various settings, usually by combining two-dimensional electrophoresis and mass spectrometry (MS) (2). Bian et al (3) recently analyzed the proteome of aorta from spontaneously hypertensive rats (SHR). They found that SHR showed a significant alteration in the aortic wall protein profile compared with normal rats.Exercise is a key antihypertensive therapy. It is reported that the physical activity level was negatively associated with blood pressure. In addition, the blood pressure can be decreased with long-term physical activity. Blood pressure of SHRs undergoing the physical activity protocol was lower than that of the normal SHRs. The functional and structural alterations in vasculature occurred in hypertensives following exercise t...

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Section: Discussionmentioning

confidence: 91%

Aortic wall proteomic analysis in spontaneously hypertensive rats with a blood pressure decrease induced by 6-week load-free swimming

Hong

Li²,

Zhang

et al. 2015

Biomedical Reports

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“…Exercise stimuli have been extensively shown to modulate the heart proteome [94, 96–102] which is normally followed by an improvement in aerobic capacity [98]. Furthermore, improved aerobic capacity is an independent factor for health status, being also inversely correlated with cardiovascular diseases [103], with exercise being a strong factor for preventing and treating hypertension and associated pathologies such as obesity and diabetes [104].…”

Section: Does Exercise Extenuate Cardiac Pathological Hypertrophy?mentioning

confidence: 99%

Effects of Hypertension and Exercise on Cardiac Proteome Remodelling

Petriz

Franco

2014

BioMed Research International

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Left ventricle hypertrophy is a common outcome of pressure overload stimulus closely associated with hypertension. This process is triggered by adverse molecular signalling, gene expression, and proteome alteration. Proteomic research has revealed that several molecular targets are associated with pathologic cardiac hypertrophy, including angiotensin II, endothelin-1 and isoproterenol. Several metabolic, contractile, and stress-related proteins are shown to be altered in cardiac hypertrophy derived by hypertension. On the other hand, exercise is a nonpharmacologic agent used for hypertension treatment, where cardiac hypertrophy induced by exercise training is characterized by improvement in cardiac function and resistance against ischemic insult. Despite the scarcity of proteomic research performed with exercise, healthy and pathologic heart proteomes are shown to be modulated in a completely different way. Hence, the altered proteome induced by exercise is mostly associated with cardioprotective aspects such as contractile and metabolic improvement and physiologic cardiac hypertrophy. The present review, therefore, describes relevant studies involving the molecular characteristics and alterations from hypertensive-induced and exercise-induced hypertrophy, as well as the main proteomic research performed in this field. Furthermore, proteomic research into the effect of hypertension on other target-demerged organs is examined.

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“…All rats were housed under a 12-h light/dark cycle with free access to food and water. The exercise training protocol consisted of swimming for 8 weeks, and the rats assigned to the exercise training group trained 6 days/week and twice daily as previously described (14). Briefly, after 1 week of familiarizing the rats to the swimming apparatus, the training period continued for 15 min during the first week, with a gradual increase in training time to 120 min in week 4; this schedule was subsequently maintained until the termination of the experiment.…”

Section: Animals and The High Intensity Training Programmentioning

confidence: 99%

High intensity training induces alteration of the ubiquitin-proteasome system gene expression profile and structural changes in the ovaries

et al. 2012

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Abstract. The female reproductive system is highly sensitive to physiological stress. However, the mechanism(s) involved in dysfunction of the ovaries induced by high intensity exercise training remain unknown. In the present study, we established a rat model of high intensity exercise training, and investigated the morphological and functional changes in ovaries. Furthermore, we profiled the differential gene expression in the ovaries between high intensity exercise training rats and control rats by restriction fragment differential display PCR. Differentially expressed genes were validated by real-time PCR. The results showed that the weight and microstructure of the ovaries were significantly altered in the exercise training group rats. In addition, the levels of estradiol, progesterone, luteinizing hormone and follicle-stimulating hormone were significantly decreased in the exercise training group rats compared with the control group rats. Immunohistochemistry revealed weak expression of estradiol, estrogen receptor and progesterone receptor in ovarian tissues from the high intensity exercise training group compared with the control group. Furthermore, the gene expression profiles of the exercise training and control group rats were analyzed, and differentially expressed genes, such as those involved in the ubiquitin-proteasome system, were identified. Our results indicate that high intensity exercise training can suppress the cellular function of the ovary glands. The mechanism may involve a series of alterations in the expression of genes particularly associated with the ubiquitin-proteasome system.

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Proteomic adaptation to chronic high intensity swimming training in the rat heart

Cited by 26 publications

References 36 publications

Aortic wall proteomic analysis in spontaneously hypertensive rats with a blood pressure decrease induced by 6-week load-free swimming

Aortic wall proteomic analysis in spontaneously hypertensive rats with a blood pressure decrease induced by 6-week load-free swimming

Effects of Hypertension and Exercise on Cardiac Proteome Remodelling

High intensity training induces alteration of the ubiquitin-proteasome system gene expression profile and structural changes in the ovaries

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