Proteome-wide identification of non-histone lysine methylation in tomato during fruit ripening

Proteome-Wide Identification of Non-histone Lysine Methylation during Grape Berry Ripening

“…34 However, no conserved motif for non-histone lysine methylation has been identified yet. Xiao et al 2 showed that two conserved nonhistone lysine methylation motifs, xxxxxxExxx_K_xxxExxxxxx and xxxxxxExxx_K_xxxxxxxxxx, are enriched in tomato fruits. In this study, we found that the xxExxx_K_xxxxxx motif is also enriched in grape berries, suggesting that xxExxx_K_xxxxxx is a conserved motif of lysine-methylated non-histone proteins; however, its reliability needs to be verified in additional plant species.…”

“…This is consistent with the results obtained in tomato. 2 To understand the process of protein modification, the identification of conserved amino acid motifs in the substrate protein targeted by upstream enzymes is generally necessary. Therefore, studying the conserved motifs of modified proteins is of great significance for predicting substrate modification sites and substrate−enzyme interactions.…”

“…Moreover, some transcription factors including NF-κB, ERα, E2F1, and RB1 are also targets of methylation. Although protein methylation plays an important role in regulating protein activity and protein–protein interactions and in the interplay with other modifications, the methylation of non-histone proteins has rarely been investigated in plants. , …”

“…1 Among these, methylation plays an indispensable role in the regulation of cell physiology, including gene expression, protein activity and stability, and signal transduction. 2 Protein methylation refers to the enzymatic transfer of a methyl group to an amino acid residue, which is usually lysine or arginine but can also be histidine, cysteine, or aspartic acid. 3 Lysine methylation, including mono-, di-, or trimethylation, is one of the most frequent epigenetic modifications.…”

“…Although protein methylation plays an important role in regulating protein activity and protein−protein interactions and in the interplay with other modifications, the methylation of non-histone proteins has rarely been investigated in plants. 2,12 Grape (Vitis vinifera L.) is an economically important fruit crop, which is valuable for studying berry fruit development and ripening. In this study, we used the 4D label-free quantitative proteomics approach to study the methylation of non-histone lysine residues in grape during berry development.…”