Proteome-wide changes in primary skin keratinocytes exposed to diesel particulate extract—A role for antioxidants in skin health

Rajagopalan, Padmavathy; Jain, Ankit; Nanjappa, Vishalakshi; Patel, Krishna; Mangalaparthi, Kiran K.; Babu, Niraj; Cavusoglu, Nükhet; Roy, Nita; Sœur, Jérémie; Breton, Lionel; Pandey, Akhilesh; Gowda, Harsha; Chatterjee, Aditi; Misra, Namita

doi:10.1016/j.jdermsci.2018.05.003

Cited by 27 publications

(28 citation statements)

References 71 publications

(29 reference statements)

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“…In 3D HEE models, PM usually in concentrations of up to 50 μg/mL was mainly applied topically and short-term exposures (i.e., 24 to 48 h) were performed [128,130,132,133]. PM was shown to affect the physical epidermal barrier in 3D models, as indicated by altered expression of proteins important for epidermal differentiation, such as filaggrin, involucrin, loricrin, and keratins [125,127,129,130,132,133]. Additionally, markers for proliferation, such as Ki67, 5-bromo-2′-deoxyuridine (BrdU) incorporation, and proliferating cell nuclear antigen (PCNA), were shown to be decreased upon PM exposure [125,130].…”

Section: Physical Barriermentioning

confidence: 99%

Impact of airborne particulate matter on skin: a systematic review from epidemiology to in vitro studies

Dijkhoff¹,

Drašler²,

Karakoçak³

et al. 2020

Part Fibre Toxicol

117

116

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Background: Air pollution is killing close to 5 million people a year, and harming billions more. Air pollution levels remain extremely high in many parts of the world, and air pollution-associated premature deaths have been reported for urbanized areas, particularly linked to the presence of airborne nano-sized and ultrafine particles. Main text: To date, most of the research studies did focus on the adverse effects of air pollution on the human cardiovascular and respiratory systems. Although the skin is in direct contact with air pollutants, their damaging effects on the skin are still under investigation. Epidemiological data suggested a correlation between exposure to air pollutants and aggravation of symptoms of chronic immunological skin diseases. In this study, a systematic literature review was conducted to understand the current knowledge on the effects of airborne particulate matter on human skin. It aims at providing a deeper understanding of the interactions between air pollutants and skin to further assess their potential risks for human health. Conclusion: Particulate matter was shown to induce a skin barrier dysfunction and provoke the formation of reactive oxygen species through direct and indirect mechanisms, leading to oxidative stress and induced activation of the inflammatory cascade in human skin. Moreover, a positive correlation was reported between extrinsic aging and atopic eczema relative risk with increasing particulate matter exposure.

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Section: Physical Barriermentioning

confidence: 99%

Impact of airborne particulate matter on skin: a systematic review from epidemiology to in vitro studies

Dijkhoff¹,

Drašler²,

Karakoçak³

et al. 2020

Part Fibre Toxicol

117

116

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“…Cellular ROS production has been shown to mediate PM‐induced cytokine production in cultured primary keratinocytes . Oxidative stress responses were also observed in HaCaT cells exposed to concentrated air particles and in primary human keratinocytes exposed to cigarette smoke condensate or diesel particulate extract . Clinical evidence for an oxidative stress response occurring in humans after exposure to pollutants comes from two prospective clinical studies comparing the impact of pollution on subjects living in Mexico City ( N = 96) and Cuernavaca ( N = 93) or in urban ( N = 79) and rural ( N = 80) areas of Shanghai .…”

Section: Biochemical Changes and Molecular Mechanisms Induced By Pollmentioning

confidence: 99%

The impact of airborne pollution on skin

Araviiskaia

Berardesca

Bieber

et al. 2019

Acad Dermatol Venereol

148

126

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Indoor and outdoor airborne pollutants modify our environment and represent a growing threat to human health worldwide. Airborne pollution effects on respiratory and cardiac health and diseases have been well established, but its impact on skin remains poorly described. Nonetheless, the skin is one of the main targets of pollutants, which reach the superficial and deeper skin layers by transcutaneous and systemic routes. In this review, we report the outcomes of basic and clinical research studies monitoring pollutant levels in human tissues including the skin and hair. We present a current understanding of the biochemical and biophysical effects of pollutants on skin metabolism, inflammatory processes and oxidative stress, with a focus on polyaromatic hydrocarbons and ground‐level ozone that are widespread outdoor pollutants whose effects are mostly studied. We reviewed the literature to report the clinical effects of pollutants on skin health and skin ageing and their impact on some chronic inflammatory skin diseases. We also discuss the potential interactions of airborne pollutants with either ultraviolet radiation or human skin microbiota and their specific impact on skin health.

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“…Diesel particulate extract (DPE) has been shown to induce cell death in different cell types, including keratinocytes (KC) [4]. We first determined what concentration of DPE alters KC growth in our experimental conditions.…”

Section: Diesel Particulate Extract (Dpe) Induces Keratinocytes (Kc) mentioning

confidence: 99%

“…Recent evidence reveals that air pollutants lead to impairment of epidermal barrier function; and diesel particulate extract (DPE) is one of the major components of air pollution, as it is the most abundant combustion-derived particle in traffic emission [2,3]. DPE consists of a heterogeneous mixture of volatile components, including aldehydes, benzene, butadiene, polyaromatic hydrocarbons (PAHs) and their derivatives [4]. Prior studies have demonstrated that cellular mechanisms underlying DPE-mediated detrimental effects are mainly initiated by the generation of oxidative stress [5].…”

Section: Introductionmentioning

confidence: 99%

NADPH Oxidase-Mediated Activation of Neutral Sphingomyelinase Is Responsible for Diesel Particulate Extract-Induced Keratinocyte Apoptosis

Lee¹,

Park

Kwon³

et al. 2020

IJMS

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Human epidermis is positioned at the interface with the external environment, protecting our bodies against external challenges, including air pollutants. Emerging evidence suggests that diesel particulate extract (DPE), a major component of air pollution, leads to impairment of diverse cellular functions in keratinocytes (KC). In this study, we investigated the cellular mechanism underlying DPE-induced KC apoptosis. We first addressed cell death occurring in KC exposed to DPE, paralleled by increased activation of NADPH oxidases (NOXs) and subsequent ROS generation. Blockade of NOX activation with a specific inhibitor attenuated the expected DPE-induced KC apoptosis. In contrast, pre-treatment with a specific inhibitor of reactive oxygen species (ROS) generation did not reverse DPE/NOX-mediated increase in KC apoptosis. We next noted that NOX-mediated KC apoptosis is mainly attributable to neutral sphingomyelinase (SMase)-mediated stimulation of ceramides, which is a well-known pro-apoptotic lipid. Moreover, we found that inhibition of NOX activation significantly attenuated DPE-mediated increase in the ratio of ceramide to its key metabolite sphingosine-1-phosphate (S1P), an important determinant of cell fate. Together, these results suggest that activation of neutral SMase serves as a key downstream signal for the DPE/NOX activation-mediated alteration in ceramide and S1P productions, and subsequent KC apoptosis.

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Proteome-wide changes in primary skin keratinocytes exposed to diesel particulate extract—A role for antioxidants in skin health

Abstract: Our study highlights distinct adverse effects of chronic exposure to DPE/DPE vapor on skin keratinocytes and the potential role of vitamin E in alleviating adverse effects of environmental pollution.

Cited by 27 publications

References 71 publications

Impact of airborne particulate matter on skin: a systematic review from epidemiology to in vitro studies

Impact of airborne particulate matter on skin: a systematic review from epidemiology to in vitro studies

The impact of airborne pollution on skin

NADPH Oxidase-Mediated Activation of Neutral Sphingomyelinase Is Responsible for Diesel Particulate Extract-Induced Keratinocyte Apoptosis

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