Proteome-wide analysis reveals molecular pathways affected by AgNP in a ROS-dependent manner

Miranda, Renata Rank; Oliveira, Anny Carolline Silva; Skytte, Lilian; Rasmussen, Kaare Lund; Kjeldsen, Frank

doi:10.1080/17435390.2022.2036844

Cited by 4 publications

(2 citation statements)

References 83 publications

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“…To evaluate whether the Ag NPs could trigger protective cellular responses in these cells, we investigated whether the Ag-B NPs obtained from Sigma triggered an antioxidant response in the BEAS-2B cell line. As shown in Supplementary Figure S8 , a clear dose-dependent upregulation of NRF2 was observed at 24 h, in line with other recent studies using different cell lines ( Miranda et al, 2022 ). However, no induction was seen in cells exposed to the TiO 2 NPs.…”

Section: Resultssupporting

confidence: 91%

Silver nanoparticles with excellent biocompatibility block pseudotyped SARS-CoV-2 in the presence of lung surfactant

Gupta

Hamawandi

Sheward

et al. 2022

Front. Bioeng. Biotechnol.

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Silver (Ag) is known to possess antimicrobial properties which is commonly attributed to soluble Ag ions. Here, we showed that Ag nanoparticles (NPs) potently inhibited SARS-CoV-2 infection using two different pseudovirus neutralization assays. We also evaluated a set of Ag nanoparticles of different sizes with varying surface properties, including polyvinylpyrrolidone (PVP)-coated and poly (ethylene glycol) (PEG)-modified Ag nanoparticles, and found that only the bare (unmodified) nanoparticles were able to prevent virus infection. For comparison, TiO2 nanoparticles failed to intercept the virus. Proteins and lipids may adsorb to nanoparticles forming a so-called bio-corona; however, Ag nanoparticles pre-incubated with pulmonary surfactant retained their ability to block virus infection in the present model. Furthermore, the secondary structure of the spike protein of SARS-CoV-2 was perturbed by the Ag nanoparticles, but not by the ionic control (AgNO3) nor by the TiO2 nanoparticles. Finally, Ag nanoparticles were shown to be non-cytotoxic towards the human lung epithelial cell line BEAS-2B and this was confirmed by using primary human nasal epithelial cells. These results further support that Ag nanoparticles may find use as anti-viral agents.

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Section: Resultssupporting

confidence: 91%

Silver nanoparticles with excellent biocompatibility block pseudotyped SARS-CoV-2 in the presence of lung surfactant

Gupta

Hamawandi

Sheward

et al. 2022

Front. Bioeng. Biotechnol.

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“…Using a NRF2/ARE Responsive Luciferase Reporter HEK293 Cell Line, it has been shown that CuO, Mn 2 O 3 and ZnO nanomaterials strongly induce the NRF2 mediated gene expression, while a recent study showed that Fe 2 O 3 materials of different sizes induced limited gene expression in these reporter cells ( Seleci et al, 2022 ). Using HEK293 cells, Ag nanomaterials have also been shown in several studies to trigger an NRF2 response in a range of different cell types ( Miranda et al, 2022 , and see other references therein).…”

Section: Introductionmentioning

confidence: 99%

Pre-validation of a reporter gene assay for oxidative stress for the rapid screening of nanobiomaterials

et al. 2022

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Engineered nanomaterials have been found to induce oxidative stress. Cellular oxidative stress, in turn, can result in the induction of antioxidant and detoxification enzymes which are controlled by the nuclear erythroid 2-related factor 2 (NRF2) transcription factor. Here, we present the results of a pre-validation study which was conducted within the frame of BIORIMA (“biomaterial risk management”) an EU-funded research and innovation project. For this we used an NRF2 specific chemically activated luciferase expression reporter gene assay derived from the human U2OS osteosarcoma cell line to screen for the induction of the NRF2 mediated gene expression following exposure to biomedically relevant nanobiomaterials. Specifically, we investigated Fe3O4-PEG-PLGA nanomaterials while Ag and TiO2 “benchmark” nanomaterials from the Joint Research Center were used as reference materials. The viability of the cells was determined by using the Alamar blue assay. We performed an interlaboratory study involving seven different laboratories to assess the applicability of the NRF2 reporter gene assay for the screening of nanobiomaterials. The latter work was preceded by online tutorials to ensure that the procedures were harmonized across the different participating laboratories. Fe3O4-PEG-PLGA nanomaterials were found to induce very limited NRF2 mediated gene expression, whereas exposure to Ag nanomaterials induced NRF2 mediated gene expression. TiO2 nanomaterials did not induce NRF2 mediated gene expression. The variability in the results obtained by the participating laboratories was small with mean intra-laboratory standard deviation of 0.16 and mean inter laboratory standard deviation of 0.28 across all NRF2 reporter gene assay results. We conclude that the NRF2 reporter gene assay is a suitable assay for the screening of nanobiomaterial-induced oxidative stress responses.

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Metal-based nanoparticles in antibacterial application in biomedical field: Current development and potential mechanisms

Jiang,

Li,

et al. 2024

Biomed Microdevices

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The rise in drug resistance in pathogenic bacteria greatly endangers public health in the post-antibiotic era, and drug-resistant bacteria currently pose a great challenge not only to the community but also to clinical procedures, including surgery, stent implantation, organ transplantation, and other medical procedures involving any open wound and compromised human immunity. Biofilm-associated drug failure, as well as rapid resistance to last-resort antibiotics, necessitates the search for novel treatments against bacterial infection. In recent years, the flourishing development of nanotechnology has provided new insights for exploiting promising alternative therapeutics for drug-resistant bacteria. Metallic agents have been applied in antibacterial usage for several centuries, and the functional modification of metal-based biomaterials using nanotechnology has now attracted great interest in the antibacterial field, not only for their intrinsic antibacterial nature but also for their ready on-demand functionalization and enhanced interaction with bacteria, rendering them with good potential in further translation. However, the possible toxicity of MNPs to the host cells and tissue still hinders its application, and current knowledge on their interaction with cellular pathways is not enough. This review will focus on recent advances in developing metallic nanoparticles (MNPs), including silver, gold, copper, and other metallic nanoparticles, for antibacterial applications, and their potential mechanisms of interaction with pathogenic bacteria as well as hosts.

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Proteome-wide analysis reveals molecular pathways affected by AgNP in a ROS-dependent manner

Cited by 4 publications

References 83 publications

Silver nanoparticles with excellent biocompatibility block pseudotyped SARS-CoV-2 in the presence of lung surfactant

Silver nanoparticles with excellent biocompatibility block pseudotyped SARS-CoV-2 in the presence of lung surfactant

Pre-validation of a reporter gene assay for oxidative stress for the rapid screening of nanobiomaterials

Metal-based nanoparticles in antibacterial application in biomedical field: Current development and potential mechanisms

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