2022
DOI: 10.1080/17435390.2022.2036844
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Proteome-wide analysis reveals molecular pathways affected by AgNP in a ROS-dependent manner

Abstract: The use of mass spectrometry-based proteomics has been increasingly applied in nanomaterials risk assessments as it provides a proteome-wide overview of the molecular disturbances induced by its exposure. Here, we used this technique to gain detailed molecular insights of the role of ROS as an effector of AgNP toxicity, by incubating Bend3 cells with AgNP in the absence or presence of an antioxidant N-acetyl L-cystein (NAC). ROS generation is a key player in AgNP-induced toxicity, as cellular homeostasis was k… Show more

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Cited by 5 publications
(3 citation statements)
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“…To evaluate whether the Ag NPs could trigger protective cellular responses in these cells, we investigated whether the Ag-B NPs obtained from Sigma triggered an antioxidant response in the BEAS-2B cell line. As shown in Supplementary Figure S8 , a clear dose-dependent upregulation of NRF2 was observed at 24 h, in line with other recent studies using different cell lines ( Miranda et al, 2022 ). However, no induction was seen in cells exposed to the TiO 2 NPs.…”
Section: Resultssupporting
confidence: 91%
“…To evaluate whether the Ag NPs could trigger protective cellular responses in these cells, we investigated whether the Ag-B NPs obtained from Sigma triggered an antioxidant response in the BEAS-2B cell line. As shown in Supplementary Figure S8 , a clear dose-dependent upregulation of NRF2 was observed at 24 h, in line with other recent studies using different cell lines ( Miranda et al, 2022 ). However, no induction was seen in cells exposed to the TiO 2 NPs.…”
Section: Resultssupporting
confidence: 91%
“…Тому, застосування НЧ є перспективним напрямком у розробці засобів протизапальної дії. Зазначимо, що НЧ набули поширеного використання в біомедичній галузі завдяки їх високій здатності проникати всередину клітин, властивостям зв'язування лігандів завдяки високому співвідношенню площі поверхні до об'єму [7][8][9]. Механістичне дослідження показало наявність протизапальної активності у різних металів і наночастинок оксидів металів, таких як Срібло, Золото тощо [1,9,10].…”
Section: сергієнко в рunclassified
“…Using a NRF2/ARE Responsive Luciferase Reporter HEK293 Cell Line, it has been shown that CuO, Mn 2 O 3 and ZnO nanomaterials strongly induce the NRF2 mediated gene expression, while a recent study showed that Fe 2 O 3 materials of different sizes induced limited gene expression in these reporter cells ( Seleci et al, 2022 ). Using HEK293 cells, Ag nanomaterials have also been shown in several studies to trigger an NRF2 response in a range of different cell types ( Miranda et al, 2022 , and see other references therein).…”
Section: Introductionmentioning
confidence: 99%