2017
DOI: 10.1038/s41598-017-17756-6
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Proteome-wide Analysis of Lysine 2-hydroxyisobutyrylation in Developing Rice (Oryza sativa) Seeds

Abstract: Lysine 2-hydroxyisobutyrylation is a recently identified protein post-translational modification that is known to affect the association between histone and DNA. However, non-histone protein lysine 2-hydroxyisobutyrylation remains largely unexplored. Utilizing antibody-based affinity enrichment and nano-HPLC/MS/MS analyses of 2-hydroxyisobutyrylation peptides, we efficaciously identified 9,916 2-hydroxyisobutyryl lysine sites on 2,512 proteins in developing rice seeds, representing the first lysine 2-hydroxyis… Show more

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Cited by 57 publications
(115 citation statements)
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“…Also in ubiquitin of wheat ( Triticum sp.) and rice, lysines were targeted by acetylation (Wang et al ., ) and by PTMs metabolically related to acetylation, such as 2‐hydroxyisobutyrylation (Meng et al ., ), succinylation (He et al ., ) and malonylation (Mujahid et al ., ). In rice, all ubiquitin lysines, except Lys29, are modified by acetylation, malonylation, butyrylation, and/or succinylation (Figure a).…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Also in ubiquitin of wheat ( Triticum sp.) and rice, lysines were targeted by acetylation (Wang et al ., ) and by PTMs metabolically related to acetylation, such as 2‐hydroxyisobutyrylation (Meng et al ., ), succinylation (He et al ., ) and malonylation (Mujahid et al ., ). In rice, all ubiquitin lysines, except Lys29, are modified by acetylation, malonylation, butyrylation, and/or succinylation (Figure a).…”
Section: Resultsmentioning
confidence: 99%
“…For instance, in plants, this post-translational N-terminal acetylation often occurs on plastid protein N-termini exposed after transit peptide cleavage (Bienvenut et al, 2012;K€ ohler et al, 2015;Rowland et al, 2015). In addition to phosphorylation and acetylation, PTMs reported of late include N-terminal myristoylation (Majeran et al, 2018), Olinked N-acetylglucosaminylation (O-GlcNAcylation) (Xu et al, 2017a) in Arabidopsis, lysine modifications, such as 2-hydroxyisobuturylation (Meng et al, 2017) and malonylation (Mujahid et al, 2018) in rice (O. sativa spp. japonica), and SUMOylation in Arabidopsis (Rytz et al, 2018).…”
Section: Introductionmentioning
confidence: 99%
“…To reveal the conservation and uniqueness of Khib among rice (Oryza sativa) [23], 234 Physcomitrella patens [41], and wheat, we utilized a BLAST search to estimate the 235 degree of conservation and uniqueness of the Khib proteins [42]. As shown in 236 Figure 5A, 752 (70.0%) of the Khib proteins were orthologous proteins between wheat 237 and the other two species (Supplemental Table S10; Figure 5A).…”
Section: Conservation and Uniqueness Of The Khib Proteins 233mentioning
confidence: 99%
“…Khib is known to occur on nuclear, cytoplasmic, mitochondrial, and chloroplast 277 proteins [23,39,40]. As a result, this PTM could be crucial for processes inside the 278 nucleus and could be important for regulating different cellular and metabolic processes.…”
Section: Khib Proteins Involved In Photosynthesis 276mentioning
confidence: 99%
“…For example, for Arabidopsis thaliana (Arabidopsis), approximately 57,000 phosphorylation sites are available in PhosPhAt 4.0 (Heazlewood et al, 2008). Besides phosphorylation, recently reported PTMs include N-terminal myristoylation (Majeran et al, 2018) and O -linked N-acetylglucosaminylation ( O -GlcNAcylation) (Xu et al, 2017) in Arabidopsis, and lysine 2-hydroxyisobuturylation (Meng et al, 2017) and malonylation (Mujahid et al, 2018) in rice ( Oryza sativa spp. japonica ).…”
Section: Introductionmentioning
confidence: 99%