Proteome-wide analysis of head and neck squamous cell carcinomas using laser-capture microdissection and tandem mass spectrometry

Enhanced Interferon Signaling Pathway in Oral Cancer Revealed by Quantitative Proteome Analysis of Microdissected Specimens Using 16O/18O Labeling and Integrated Two-dimensional LC-ESI-MALDI Tandem MS

“…However, relatively few studies have used these techniques to identify OSCC/HNSCC-associated proteins in tissue specimens from patients with OSCC/HNSCC (15,17,29). For example, Melle et al (29) used ProteinChip technology and 2D gel electrophoresis to examine the up-regulation of annexin V in microdissected HNSCC tissues.…”

“…For example, Melle et al (29) used ProteinChip technology and 2D gel electrophoresis to examine the up-regulation of annexin V in microdissected HNSCC tissues. In addition, Baker et al (17) used LC-MS/MS to identify ϳ100 unique proteins in sets of normal and cancerous microdissected tongue specimens and used immunohistochemistry to demonstrate the downregulation of cytokeratin (CK) 13 and the up-regulation of heat-shock protein 90 in tumor cells. Another recent study used LC-MS/MS to analyze the proteins extracted from microdissected formalin-fixed, paraffin-embedded tissue sections of normal oral epithelium as well as well, moderately, and poorly differentiated oral cancers.…”

“…Recently state-of-the-art proteomics technologies have revealed alterations in protein abundance, posttranslational modification and turnover, and spatial and temporal distribution within tumor specimens. Using proteomics approaches, aberrantly expressed proteins have been identified in body fluids (12)(13)(14), frozen or paraffin-embedded tissues (15)(16)(17)(18), and cultured cell lines (19 -22). The fold changes in protein expression in samples from healthy and cancerous states as well as the roles of each protein in disease progression must be determined to identify potential candidates for biomarkers and therapeutic targets.…”

Network-based analysis of calcium-binding protein genes identifies Grp94 as a target in human oral carcinogenesis

“…Considerable evidence has shown a significant association between the heat-shock protein 90 (Hsp90) and a wide range of human malignancies, including head and neck cancers (Ito et al, 1998;Baker et al, 2005;Yin et al, 2005). Grp94, also known as gp96, is the ER-resident member of the Hsp90 family constitutively expressed in virtually all cell types (Van et al, 1989) and the most abundant ER chaperon protein showing high homology (50%) to cytosolic counterpart Hsp90 (Sorger and Pelham, 1987).…”

Tissue Proteomics for the Next Decade? Towards a Molecular Dimension in Histology

“…Label free LC-MS approaches have been used to study several cancers like head and neck squamous cell carcinomas (Baker et al, 2005), esophageal cancer (Hatakeyama et al, 2006), dysplasic cervical cells TISSUE PROTEOMICS 5 (Gu et al, 2007), breast carcinoma tumors (Hill et al, 2011;Johann et al, 2009), tamoxifen-resistant breast cancer cells (Umar et al, 2009), ER + / -breast cancer cells (Rezaul et al, 2010), Barretts esophagus (Stingl et al, 2011), and ovarian endometrioid cancer (Alkhas et al, 2011). Different isotope labeling methods have been used in order to compare proteins expression.…”