Proteome-Wide Analysis and Diel Proteomic Profiling of the Cyanobacterium Arthrospira platensis PCC 8005

Matallana-Surget, Sabine; Derock, Jérémy; Leroy, Baptiste; Badri, Hanène; Deschoenmaeker, Frédéric; Wattiez, Ruddy

doi:10.1371/journal.pone.0099076

Cited by 28 publications

(27 citation statements)

References 43 publications

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“…4). As far as we are aware, these proteins were never been reported as significantly expressed in Arthrospira in response to any other stress condition tested such as light stress (Matallana-Surget et al 2014) or nitrogen deficiency) , which makes their response to IR rather unique and peculiar. It is possible that this set of genes may play an important role in the high radiation resistance of Arthrospira sp.…”

Section: Discussionmentioning

confidence: 89%

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Molecular investigation of the radiation resistance of edible cyanobacterium Arthrospira sp. PCC 8005

et al. 2015

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The aim of this work was to characterize in detail the response of Arthrospira to ionizing radiation, to better understand its radiation resistance capacity. Live cells of Arthrospira sp. PCC 8005 were irradiated with 60Co gamma rays. This study is the first, showing that Arthrospira is highly tolerant to gamma rays, and can survive at least 6400 Gy (dose rate of 527 Gy h−1), which identified Arthrospira sp. PCC 8005 as a radiation resistant bacterium. Biochemical, including proteomic and transcriptomic, analysis after irradiation with 3200 or 5000 Gy showed a decline in photosystem II quantum yield, reduced carbon fixation, and reduced pigment, lipid, and secondary metabolite synthesis. Transcription of photo-sensing and signaling pathways, and thiol-based antioxidant systems was induced. Transcriptomics did show significant activation of ssDNA repair systems and mobile genetic elements (MGEs) at the RNA level. Surprisingly, the cells did not induce the classical antioxidant or DNA repair systems, such superoxide dismutase (SOD) enzyme and the RecA protein. Arthrospira cells lack the catalase gene and the LexA repressor. Irradiated Arthrospira cells did induce strongly a group of conserved proteins, of which the function in radiation resistance remains to be elucidated, but which are a promising novel routes to be explored. This study revealed the radiation resistance of Arthrospira, and the molecular systems involved, paving the way for its further and better exploitation.

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Section: Discussionmentioning

confidence: 89%

“…As far as we are aware, these proteins were never been reported as significantly expressed in Arthrospira in response to any other stress condition tested such as light stress (Matallana‐Surget et al. ) or nitrogen deficiency) (Deschoenmaeker et al. ), which makes their response to IR rather unique and peculiar.…”

Section: Discussionmentioning

confidence: 91%

Molecular investigation of the radiation resistance of edible cyanobacterium Arthrospira sp. PCC 8005

et al. 2015

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“…So far these proteins were not differentially induced in Arthrospira sp. PCC 8005 in response to other stresses, such as nitrogen deprivation [ 84 ] or light/dark growth transition [ 85 ]. These findings suggest a clear correlation between this gene set and the resistance of Arthospira sp.…”

Section: Discussionmentioning

confidence: 99%

Temporal Gene Expression of the Cyanobacterium Arthrospira in Response to Gamma Rays

et al. 2015

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The edible cyanobacterium Arthrospira is resistant to ionising radiation. The cellular mechanisms underlying this radiation resistance are, however, still largely unknown. Therefore, additional molecular analysis was performed to investigate how these cells can escape from, protect against, or repair the radiation damage. Arthrospira cells were shortly exposed to different doses of 60Co gamma rays and the dynamic response was investigated by monitoring its gene expression and cell physiology at different time points after irradiation. The results revealed a fast switch from an active growth state to a kind of 'survival modus' during which the cells put photosynthesis, carbon and nitrogen assimilation on hold and activate pathways for cellular protection, detoxification, and repair. The higher the radiation dose, the more pronounced this global emergency response is expressed. Genes repressed during early response, suggested a reduction of photosystem II and I activity and reduced tricarboxylic acid (TCA) and Calvin-Benson-Bassham (CBB) cycles, combined with an activation of the pentose phosphate pathway (PPP). For reactive oxygen species detoxification and restoration of the redox balance in Arthrospira cells, the results suggested a powerful contribution of the antioxidant molecule glutathione. The repair mechanisms of Arthrospira cells that were immediately switched on, involve mainly proteases for damaged protein removal, single strand DNA repair and restriction modification systems, while recA was not induced. Additionally, the exposed cells showed significant increased expression of arh genes, coding for a novel group of protein of unknown function, also seen in our previous irradiation studies. This observation confirms our hypothesis that arh genes are key elements in radiation resistance of Arthrospira, requiring further investigation. This study provides new insights into phasic response and the cellular pathways involved in the radiation resistance of microbial cells, in particularly for photosynthetic organisms as the cyanobacterium Arthrospira.

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“…Protein synthesis and biomass accumulation measurements show similar patterns in Synechococcus spp. (Glover and Smith,390 1988), the unicellular red alga Cyanidioschyzon merolae (Miyagishima et al, 2014), the cyanobacterium Arthrospira plantensis (Matallana-Surget et al, 2014), and the marine diatom Thalassiosira pseudonana (Ashworth et al, 2013). Diel and circadian regulation of the transcriptome has also been tested.…”

Section: Translational Coincidence As a General Mechanism Of Photopermentioning

confidence: 99%

Photoperiodic control of the Arabidopsis proteome reveals a translational coincidence mechanism

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Baerenfaller

et al. 2017

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Plants respond to seasonal cues, such as the photoperiod, to adapt to current conditions and to prepare for environmental changes in the season to come. To assess photoperiodic responses at the protein level, we quantified the proteome of the model plant Arabidopsis thaliana by mass spectrometry across four 35 photoperiods. This revealed coordinated changes of abundance in proteins of photosynthesis, primary and secondary metabolism, including pigment biosynthesis, consistent with higher metabolic activity in long photoperiods. Higher translation rates in the daytime than the night likely contribute to these changes via rhythmic changes in RNA abundance. Photoperiodic control of protein levels might be greatest only if high translation rates coincide with high transcript levels in some photoperiods. We term this proposed 40 mechanism 'translational coincidence', mathematically model its components, and demonstrate its effect on the Arabidopsis proteome. Datasets from a green alga and a cyanobacterium suggest that translational coincidence contributes to seasonal control of the proteome in many phototrophic organisms. This may explain why many transcripts but not their cognate proteins exhibit diurnal rhythms. 45Keywords:Proteomics / circadian rhythms / metabolism / photoperiod / seasonality . CC-BY 4.0 International license not peer-reviewed) is the author/funder. It is made available under a

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Proteome-Wide Analysis and Diel Proteomic Profiling of the Cyanobacterium Arthrospira platensis PCC 8005

Cited by 28 publications

References 43 publications

Molecular investigation of the radiation resistance of edible cyanobacterium Arthrospira sp. PCC 8005

Molecular investigation of the radiation resistance of edible cyanobacterium Arthrospira sp. PCC 8005

Temporal Gene Expression of the Cyanobacterium Arthrospira in Response to Gamma Rays

Photoperiodic control of the Arabidopsis proteome reveals a translational coincidence mechanism

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