Proteome variation of the rat liver after static cold storage assayed in an ex vivo model

Knecht, Camila A.; Balaban, Cecilia Lucía; Rodriguez, Joaquı́n V.; Ceccarelli, Eduardo A.; Guibert, Edgardo E.; Rosano, Germán L.

doi:10.1016/j.cryobiol.2018.10.003

Cited by 3 publications

(2 citation statements)

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“…Fifty percent of the studies were from Asia, 27% were from the US, and 18% were from Europe. Only one paper was from South America (16). The selected studies were published between 2004 and 2019, with 40% published in the last 5 years (Table 1).…”

Section: Literature Search and Studies Descriptionsmentioning

confidence: 99%

“…Three other studies recollected both ischemia and reperfusion samples and compared them to control (pre- ischemia) samples and even between them (18,29,36). When rats were used as a model, Knecht et al (16) carried out ex vivo cold storage and normothermic reperfusion, whereas the other 2 publications performed a model of in vivo selective warm ischemia using vascular clamping followed by reperfusion, such in normal (20) as in steatotic rats (27).…”

Section: Sample Sources and Study Typesmentioning

confidence: 99%

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Proteomics in Liver Transplantation: A Systematic Review

et al. 2021

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BackgroundAlthough proteomics has been employed in the study of several models of liver injury, proteomic methods have only recently been applied not only to biomarker discovery and validation but also to improve understanding of the molecular mechanisms involved in transplantation.MethodsThe study was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) methodology and the guidelines for performing systematic literature reviews in bioinformatics (BiSLR). The PubMed, ScienceDirect, and Scopus databases were searched for publications through April 2020. Proteomics studies designed to understand liver transplant outcomes, including ischemia-reperfusion injury (IRI), rejection, or operational tolerance in human or rat samples that applied methodologies for differential expression analysis were considered.ResultsThe analysis included 22 studies after application of the inclusion and exclusion criteria. Among the 497 proteins annotated, 68 were shared between species and 10 were shared between sample sources. Among the types of studies analyzed, IRI and rejection shared a higher number of proteins. The most enriched pathway for liver biopsy samples, IRI, and rejection was metabolism, compared to cytokine-cytokine receptor interactions for tolerance.ConclusionsProteomics is a promising technique to detect large numbers of proteins. However, our study shows that several technical issues such as the identification of proteoforms or the dynamic range of protein concentration in clinical samples hinder the successful identification of biomarkers in liver transplantation. In addition, there is a need to minimize the experimental variability between studies, increase the sample size and remove high-abundance plasma proteins.

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Section: Literature Search and Studies Descriptionsmentioning

confidence: 99%