Proteome study of colorectal carcinogenesis

Stulı́k, Jiřı́; Hernychová, Lenka; Porkertová, Stanislava; Knížek, Jiří; Macela, Aleš; Bureš, Jan; Jandı́k, P; Langridge, J.; Jungblut, Peter R.

doi:10.1002/1522-2683(200108)22:14<3019::aid-elps3019>3.0.co;2-t

Cited by 72 publications

(65 citation statements)

References 45 publications

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“…However, the biological function of this protein is still unclear. PHB protein is overexpressed in CRC tissues compared with non-cancer tissue by two-dimensional gel electrophoresis (11,12). Additional large sample, clinical studies are needed to determine the role of PHB in tumorigenesis and development of CRC before PHB can be used as a biomarker for disease based solely on proteomics.…”

Section: Introductioncontrasting

confidence: 55%

Identification of prohibitin as a potential biomarker for colorectal carcinoma based on proteomics technology

Chen

Chen²,

Lu³

et al. 2010

Int J Oncol

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Abstract. Differential protein expression was analyzed in carcinoma tissue to determine the correlation between protein levels and the clinical and pathological parameters of patients with colorectal carcinomas (CRC). Two-dimensional electrophoresis (2-DE) revealed 40 protein spots that were differentially expressed at two or greater fold difference in CRC that were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/TOF MS). Among these proteins, prohibitin (PHB) was found to be overexpressed in CRC. It was selected for Western blot and immunohistochemistry assay in subsequent tissue microassays (TMA). Thirty-five distinct proteins were differentially expressed at least 2-fold among normal and CRC tissues. The expression of 17 proteins, including PHB, was increased by >2-fold in CRC tissues (p<0.01). Immunohistochemistry and Western blot assays found that PHB was overexpressed in CRC cases, and the expression was higher than adenoma and normal tissues (p<0.01), whereas there was no significant difference in expression of PHB between adenoma and normal tissues. Immunohistochemistry also suggested a link between PHB expression and poor differentiation (p<0.01). However, there was no difference in UICC stage, or location of CRC. Survival analysis suggested no significant correlation between PHB expression and poor prognosis. In summary, the overexpression of PHB might be associated with the transformation process from adenoma to CRC. Further, it is a potential diagnostic and differentiation biomarker of CRC in the clinic.

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Section: Introductioncontrasting

confidence: 55%

Identification of prohibitin as a potential biomarker for colorectal carcinoma based on proteomics technology

Chen

Chen²,

Lu³

et al. 2010

Int J Oncol

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“…Moreover, silver-stained protein spots tend to be difficult to identify proteins by mass spectrometry because each spot often contains small amount of protein and the proteins also are modified by formaldehyde used as a fixative. For example, a previous proteomic study of primary colorectal cancer using IPGs and silver staining identified only 18 of 57 (32%) proteins, whereas we were able to identify 97 of 107 (90.7%) proteins in this study (14). Although recent progress of mass spectrometry makes it possible to detect small amount of proteins, it is safe to start with a large amount of protein to identify proteins efficiently.…”

Section: Discussionmentioning

confidence: 91%

“…Although extensive proteome analysis has identified numerous proteins overexpressed in various cancer tissues, few markers have been accepted for routine clinical use because of conflicting reports or because potential candidates have not been detected because of their low abundance (6). Several investigators have extensively performed proteome studies of colorectal cancer; however, a limited number of proteins have been identified (11)(12)(13)(14)(15). Thus, it is necessary to develop novel techniques that permit large-scale quantitative comparisons of protein expression between normal and cancer tissues.…”

Section: Introductionmentioning

confidence: 99%

Identification of Altered Protein Expression and Post-Translational Modifications in Primary Colorectal Cancer by Using Agarose Two-Dimensional Gel Electrophoresis

Tomonaga¹,

Matsushita

Yamaguchi³

et al. 2004

Clinical Cancer Research

128

129

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Purpose: Although numerous proteome studies have been performed recently to identify cancer-related changes in protein expression, only a limited display of relatively abundant proteins has been identified. The aim of this study is to identify novel proteins as potential tumor markers in primary colorectal cancer tissues using a high-resolution two-dimensional gel electrophoresis (2-DE).Experimental Design: 2-DE using an agarose gel for isoelectric focusing was used to compare protein profiling of 10 colorectal cancer tissues and adjacent normal mucosa. Altered expression and post-translational modification of several proteins were examined using Western blot analysis and immunohistochemistry.Results: Ninety-seven proteins of 107 spots (90.7%) that were differentially expressed between matched normal and tumor tissues were identified by mass spectrometry. Among them, 42 unique proteins (49 spots) significantly increased or decreased in the tumors. They include eukaryotic translation initiation factor 4H, inorganic pyrophosphatase, anterior gradient 2 homologue, aldolase A, and chloride intracellular channel 1, whose elevated expression in tumor tissues was confirmed by Western blot analysis and immunohistochemistry. Interestingly, only isoform 1 of two transcript variants of eukaryotic translation initiation factor 4H was greatly up-regulated in most of the tumor tissues.Moreover, post-translational modifications of the prolyl-4-hydroxylase ␤ subunit and annexin A2 also were identified.Conclusions: We identified several novel proteins with altered expression in primary colorectal cancer using agarose 2-DE. This method is a powerful technique with which to search for not only quantitative but also qualitative changes in a biological process of interest and may contribute to the deeper understanding of underlying mechanisms of human cancer.

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“…We, therefore, aimed to identify the radiationresponsive genes at the protein level using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and matrix assisted laser desorption/ionization time of flight-mass spectrometry (MALDI-TOF-MS) in MCF-7 human breast cancer cells. Tools such as 2D-PAGE and MALDI-TOF-MS have been widely used for the study of cancer proteomics, as noted in several other studies (23)(24)(25).…”

Section: Introductionmentioning

confidence: 99%

Protein expression pattern in response to ionizing radiation in MCF-7 human breast cancer cells

Jung

Lee

et al. 2011

Oncology Letters

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Abstract. Breast cancer is one of the most common types of cancer in women and is highly treatable by radiotherapy. However, repeated exposure to radiation results in tumor cell resistance. Understanding the molecular mechanisms involved in the response of tumors to γ-irradiation is important for improving radiotherapy. For this reason, we aimed to identify radiation-responsive genes at the protein level. In the present study, we observed differentially expressed proteins using 2D-PAGE and MALDI-TOF-MS for the global analysis of protein expression patterns in response to ionizing radiation (IR). When the expression patterns of proteins were compared to a control gel, numerous spots were found that differed greatly. Among them, 11 spots were found to be significantly different. One set of proteins (GH2, RGS17, BAK1, CCNH, TSG6, RAD51B, IGFBP1 and CASP14) was upregulated and another set of proteins (C1QRF, PLSCR2 and p34 SE1-1 ) was downregulated after exposure to γ-rays. These proteins are known to be related to cell cycle control, apoptosis, DNA repair, cell proliferation and other signaling pathways.

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Proteome study of colorectal carcinogenesis

Cited by 72 publications

References 45 publications

Identification of prohibitin as a potential biomarker for colorectal carcinoma based on proteomics technology

Identification of prohibitin as a potential biomarker for colorectal carcinoma based on proteomics technology

Identification of Altered Protein Expression and Post-Translational Modifications in Primary Colorectal Cancer by Using Agarose Two-Dimensional Gel Electrophoresis

Protein expression pattern in response to ionizing radiation in MCF-7 human breast cancer cells

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