Proteome Response of<i>Monascus pilosus</i>during Rice Starch Limitation with Suppression of Monascorubramine Production

Lin, Wun-Yuan; Chang, Jer-Haur; Hish, Chih-Hsuan; Pan, Tzu‐Ming

doi:10.1021/jf071109u

Cited by 4 publications

(2 citation statements)

References 52 publications

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“…According to the above, the formation of secondary metabolites is very close to the health function of Monascus -fermented product. Culture conditions or the components added would significantly affect protein expression and levels of the secondary metabolites of Monascus species. − However, production of the functional hypolipidemic ingredients of Monascus -fermented product including monacolin K, monascin, and ankaflavin was increased by supplementation of the special components to stimulate the hypolipidemic effect . Meanwhile, citrinin, known as the mycotoxin involving damage of the liver and kidney, was found in RMR .…”

Section: Introductionmentioning

confidence: 99%

Enhanced Hypolipidemic Effect and Safety of Red Mold Dioscorea Cultured in Deep Ocean Water

Lee

Kung

Wang

et al. 2011

J. Agric. Food Chem.

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Red mold dioscorea (RMD) produced by Monascus sp. was proven to be a hypolipidemic functional food. Deep ocean water (DOW), that is, water obtained from over 200 m deep in the ocean, was found to promote the growth of fungus via its mineral richness. On the basis of the advantages, this study used 650 m DOW as the culture water to culture Monascus purpuresus NTU 568 and produce the DOW-RMD. The goal of this study is to compare the difference between DOW-RMD and reverse osmosis water-cultured RMD (ROW-RMD) on the hypolipidemic effect. Hyperlipidemic hamsters were fed a high-cholesterol diet and administered various doses of DOW-RMD or ROW-RMD for 8 weeks. After sacrifice, biochemical analyses in serum, liver, and feces were carried out. The results showed that DOW-RMD had a greater effect on lowering cholesterol levels and lipid peroxidation in serum and lipid plaque in heart aorta than ROW-RMD. However, DOW was likely to modulate the Monascus metabolite biosynthesis pathway toward the formation of hypolipidemic yellow pigments (such as monascin and ankaflavin) rather than red pigments and the mycotoxin citrinin. In addition, the DOW with higher Mg(2+) ion was proven to absorb into DOW-RMD; however, the accumulation of Mg(2+) ions should contribute a greater hypolipidemic effect to DOW-RMD. Comprehensively, the DOW-induced metabolism modulation and the ions of DOW were a benefit to the development of safe DOW-RMD with low citrinin levels and high hypolipidemic, antiatherosclerosis, and anti-fatty liver effects.

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Section: Introductionmentioning

confidence: 99%

Enhanced Hypolipidemic Effect and Safety of Red Mold Dioscorea Cultured in Deep Ocean Water

Lee

Kung

Wang

et al. 2011

J. Agric. Food Chem.

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“…However, compared to other filamentous fungi such as Aspergillus and Penicillium , besides a few 2-DE, studies have been performed on Monascus , and the quantitative and qualitative proteomic studies of Monascus are very few . − More importantly, accurate quantification and large-scale identification of differentially expressed proteins cannot be provided by 2-DE and iTRAQ proteomics approach. Now, SWATH-MS (sequential window acquisition of all theoretical mass spectra–mass spectrometry) can acquire data by repeatedly cycling through sequential mass windows over the whole chromatographic elution range generating a complete recording of all analyses in the sample and collect data on the low-intensity proteins that are commonly missed in data-dependent acquisition (DDA) methods .…”

Section: Introductionmentioning

confidence: 99%

Quantitative Proteomics Analysis by Sequential Window Acquisition of All Theoretical Mass Spectra–Mass Spectrometry Reveals Inhibition Mechanism of Pigments and Citrinin Production of Monascus Response to High Ammonium Chloride Concentration

Zhou

Tian

et al. 2019

J. Agric. Food Chem.

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Various Monascus bioactive metabolites used as food or food additives in Asia for centuries are subjected to constant physical and chemical changes and different Monascus genus. With the aim to identify enzymes that participate in or indirectly regulate the pigments and citrinin biosynthesis pathways of Monascus purpureus cultured under high ammonium chloride, the changes of the proteome profile were examined using sequential window acquisition of all theoretical mass spectra–mass spectrometry-based quantitative proteomics approach in combination with bioinformatics analysis. A total of 292 proteins were confidently detected and quantified in each sample, including 163 that increased and 129 that decreased (t-tests, p ≤ 0.05). Pathway analysis indicated that high ammonium chloride in the present study accelerates the carbon substrate utilization and promotes the activity of key enzymes in glycolysis and β-oxidation of fatty acid catabolism to generate sufficient acetyl-CoA. However, the synthesis of the monascus pigments and citrinin was not enhanced because of inhibition of the polyketide synthase activity. All results demonstrated that the cause of initiation of pigments and citrinin synthesis is mainly due to the apparent inhibition of acyl and acetyl transfer by some acyltransferase and acetyltransferase, likely malony-CoA:ACP transacylase.

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Proteome analysis reveals global response to deletion of mrflbA in Monascus ruber

et al. 2018

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Monascus spp. are commonly used for a wide variety of applications in the food and pharmaceutical industries. In previous studies, the knock-out of mrflbA (a putative regulator of the G protein α subunit) in M. ruber led to autolysis of the mycelia, decreased pigmentation and lowered mycotoxin production. Therefore, we aimed to obtain a comprehensive overview of the underlying mechanism of mrflbA deletion at the proteome level. A two-dimensional gel electrophoresis analysis of mycelial proteins indicated that the abundance of 178 proteins was altered in the ΔmrflbA strain, 33 of which were identified with high confidence. The identified proteins are involved in a range of activities, including carbohydrate and amino acid metabolism, hyphal development and the oxidative stress response, protein modification, and the regulation of cell signaling. Consistent with these findings, the activity of antioxidative enzymes and chitinase was elevated in the supernatant of the ΔmrflbA strain. Furthermore, deletion of mrflbA resulted in the transcriptional reduction of secondary metabolites (pigment and mycotoxin). In short, the mutant phenotypes induced by the deletion of mrflbA were consistent with changes in the expression levels of associated proteins, providing direct evidence of the regulatory functions mediated by mrflbA in M. ruber.

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Proteome Response ofMonascus pilosusduring Rice Starch Limitation with Suppression of Monascorubramine Production

Cited by 4 publications

References 52 publications

Enhanced Hypolipidemic Effect and Safety of Red Mold Dioscorea Cultured in Deep Ocean Water

Enhanced Hypolipidemic Effect and Safety of Red Mold Dioscorea Cultured in Deep Ocean Water

Quantitative Proteomics Analysis by Sequential Window Acquisition of All Theoretical Mass Spectra–Mass Spectrometry Reveals Inhibition Mechanism of Pigments and Citrinin Production of Monascus Response to High Ammonium Chloride Concentration

Proteome analysis reveals global response to deletion of mrflbA in Monascus ruber

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