Proteome of Plasmopara viticola -infected Vitis vinifera provides insights into grapevine Rpv1 / Rpv3 pyramided resistance to downy mildew

Nascimento-Gavioli, Maria Carolina Andrade; Agapito-Tenfen, Sarah Zanon; Nodari, Rubens Onofre; Welter, Leocir José; Sánchez-Mora, Fernando David; Saifert, Luciano; Silva, Aparecido Lima da; Guerra, Miguel Pedro

doi:10.1016/j.jprot.2016.05.024

Cited by 19 publications

(11 citation statements)

References 65 publications

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“…assimilation was noticed few hours after pathogen contact with its host, and such a decrease remained up to 12 days after P. viticola inoculation in resistant cultivars Solaris, Cabernet Blanc and Phoenix. This behavior could result from the downregulation of proteins related to photosynthesis and carbohydrate metabolism as previously reported in resistant grapevine cultivars (Figueiredo et al, 2017;Nascimento-Gavioli et al, 2017). A fast and massive transcription of signaling genes affected by P. viticola has also been reported on Rpv-bearing in grapevine cultivars (Fröbel et al, 2019).…”

Section: Discussionsupporting

confidence: 67%

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Photosynthetic Cost Associated With Induced Defense to Plasmopara viticola in Grapevine

et al. 2020

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Downy mildew caused by Plasmopara viticola is one of the most destructive diseases of Vitis vinifera worldwide. Grapevine breeding programs have introgressed P. viticolaresistant traits into cultivated V. vinifera genotypes and launched interspecific hybrids with resistance against downy mildew. In general, pathogen infection affects primary metabolism, reduces plant growth and development and modifies the secondary metabolism toward defense responses, which are costly in terms of carbon production and utilization. The objective of this work was to evaluate the photosynthesis impairment by inducible defenses at the leaf level in V. vinifera cultivars resistant to P. viticola. Photosynthetic limitations imposed by P. viticola in susceptible and resistant grapevine cultivars were evaluated. Histochemical localization of hydrogen peroxide and superoxide and the activity of ascorbate peroxidase were assessed. Measurements of leaf gas exchange, chlorophyll fluorescence and the response of leaf CO 2 assimilation to increasing air CO 2 concentrations were taken, and photosynthetic limitations determined in cultivars Solaris (resistant) and Riesling (susceptible). The net photosynthetic rates were reduced (−25%) in inoculated Solaris plants even before the appearance of cell death-like hypersensitive reactions ("HR"). One day after "HR" visualization, the net photosynthetic rate of Solaris was reduced by 57% compared with healthy plants. A similar pattern was noticed in resistant Cabernet Blanc and Phoenix plants. While the susceptible cultivars did not show any variation in leaf gas exchange before the appearance of visual symptoms, drastic reductions in net photosynthetic rate and stomatal conductance were found in diseased plants 12 days after inoculation. Decreases in the maximum Rubisco carboxylation rate and photochemical impairment were noticed in Riesling after inoculation with P. viticola, which were not found in Solaris. Damage to the photochemical reactions of photosynthesis was likely associated with the oxidative burst found in resistant cultivars within the first 24 h after inoculation. Both chlorophyll degradation and stomatal closure were also noticed in the incompatible interaction. Taken together, our data clearly revealed that the defense response against P. viticola causes a photosynthetic cost to grapevines, which is not reversible even 12 days after the pathogen infection.

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Section: Discussionsupporting

confidence: 67%

“…Expression of plant defenses is often assumed to be costly, requiring diversion of resources away from plant growth and development (Walters and Boyle, 2005), and P. viticola greatly enhances carbohydrate hydrolysis and represses photosynthesis-associated proteins in resistant cultivars (Nascimento-Gavioli et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Photosynthetic Cost Associated With Induced Defense to Plasmopara viticola in Grapevine

et al. 2020

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“…The increasing number of infection responsive proteins during inoculation suggests that, although larger transcriptional changes occur as soon as 6 hpi [24], a larger proteome modulation occurs with the growing contact between pathogen and plant. In fact, other studies also report an increase number of P. viticola responsive proteins along the inoculation time-course [21,22]. 84 of the identified proteins were differentially accumulated in both time-points.…”

Section: Proteomic Modulation In Grapevine Leaves In the First Hours mentioning

confidence: 75%

“…A clear difference between pre-and post-infection stages was reported when comparing protein modulation in compatible and incompatible interactions [20,21]. The incompatible interaction in Rpv1 and Rpv3 pyramided resistance loci genotypes is characterized by a high accumulation of redox, energy and defence response proteins [22]. In previous studies, we have characterized the genotype-specific responses to P. viticola inoculation by direct comparison of a tolerant (Rpv3.1 background) with a susceptible grapevine genotype using a 2D-DIGE approach.…”

Section: Introductionmentioning

confidence: 99%

Grapevine – Downy Mildew <em>Rendez-Vous</em>: Proteome Analysis of the First Hours of an Incompatible Interaction

Santos¹,

Nascimento²,

Coelho³

et al. 2020

Preprint

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Grapevine is one of the most relevant crops in the world being used for economically important products such as wine. Yet, relevant grapevine cultivars are heavily affected by diseases such as the downy mildew disease caused by Plasmopara viticola. Improvements on grapevine resistance are made mainly by breeding techniques where resistance traits are introgressed into cultivars with desired grape characteristics. However, there is still a lack of knowledge on how resistant or tolerant cultivars tackle the P. viticola pathogen. In this study, using a shotgun proteomics LC-MS/MS approach, we unravel the protein modulation of a highly tolerant grapevine cultivar, V. vinifera ‘Regent’, in the first hours post inoculation (hpi) with P. viticola. At 6 hpi, proteins related to defence and to response to stimuli are negatively modulated while at 12 hpi there is an accumulation of proteins belonging to both categories. The co-occurrence of effector-triggered susceptibility (ETS) and effector-triggered immunity (ETI) is detected at both time-points, showing that these defence processes present high plasticity. The results obtained in this study unravel the tolerant grapevine defence strategy towards P. viticola and may provide valuable insights on resistance associated candidates and mechanisms, which may play an important role in the definition of new strategies on breeding approaches.

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“…The age of metabolomics arrived in recent decades after genomics and proteomics dominated in biomedical sciences due to their applications [1]. Powerful techniques in the later cases such as transcript analysis, protein expression studies were useful to determine the molecular mechanism of biological phenomena at genomics and proteomics level [2,3]. During those past periods, biochemistry had very least role although the analyses of metabolites using individual conventional biochemical techniques had been used from past centuries.…”

Section: Introductionmentioning

confidence: 99%

Mass Spectrophotometry: An Advanced Technique in Biomedical Sciences

Paital¹

2015

Adv Tech Biol Med

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Present century is the era of life sciences and metabolomics is the dominating field in present day over genomics and proteomics. Powerful techniques in the later cases are useful to determine the qualitative and quantitative studies of the levels of metabolites in bio-medical samples. In past time, biochemistry had very least role because the analyses of metabolites using individual conventional biochemical techniques had been used from past centuries. Owing to this limitation, mass spectrometry (MS) was introduced in bio-medical sciences. The fundamental rule in MS is that it ionizes individual chemical species and sorts the fragmented samples at charged state (ions) on the basis of their mass to charge (m/z) ratio. Therefore, each molecule in purified form or in homogenate can be identified and quantified based on their unique m/z ratio. In other words, MS measures the masses of the fragmented samples by enabling them to be charged during measurement. This particular technique has therefore, many applications starting from quantitative to qualitative analyses of metabolites under normal, experimental and diseased conditions in organisms including human being. Based on the method applied, sample preparation process, sample type, measurement process such as abundance or time of retention or flight of charged fragments etc., each MS vary with its own advantages and disadvantages. A general commentary article is written on this particular modern technique to make it understood and popular among the researchers.

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Proteome of Plasmopara viticola -infected Vitis vinifera provides insights into grapevine Rpv1 / Rpv3 pyramided resistance to downy mildew

Cited by 19 publications

References 65 publications

Photosynthetic Cost Associated With Induced Defense to Plasmopara viticola in Grapevine

Photosynthetic Cost Associated With Induced Defense to Plasmopara viticola in Grapevine

Grapevine – Downy Mildew <em>Rendez-Vous</em>: Proteome Analysis of the First Hours of an Incompatible Interaction

Mass Spectrophotometry: An Advanced Technique in Biomedical Sciences

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