Proteome of olive non-glandular trichomes reveals protective protein network against (a)biotic challenge

Roka, Loukia; Κουδούνας, Κωνσταντίνος; Δάρας, Γεράσιμος; Zoidakis, Jérôme; Vlahou, Antonia; Kalaitzis, Panagiotis; Hatzopoulos, Polydefkis

doi:10.1016/j.jplph.2018.09.016

Cited by 17 publications

(11 citation statements)

References 72 publications

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“…Recently, molecular studies confirmed that mature olive trichomes are transcriptionally active, coding mainly for enzymes catalysing reactions involved in the biosynthesis of phenolics playing important protective and defensive roles (Koudounas et al 2015). Roka et al (2018) identified 249 proteins from olive mature trichomes which were classified to diverse groups such as "phosphorylation", "response to stress" and "carbohydrate metabolic process" indicating that the cells of these structures are physiologically and biochemically active.…”

Section: Non-glandular Trichomes Contain Phenolics Associated With Cell Wallsmentioning

confidence: 99%

Protective and defensive roles of non-glandular trichomes against multiple stresses: structure–function coordination

et al. 2019

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As superficial structures, non-glandular trichomes, protect plant organs against multiple biotic and abiotic stresses. The protective and defensive roles of these epidermal appendages are crucial to developing organs and can be attributed to the excellent combination of suitable structural traits and chemical reinforcement in the form of phenolic compounds, primarily flavonoids. Both the formation of trichomes and the accumulation of phenolics are interrelated at the molecular level. During the early stages of development, non-glandular trichomes show strong morphological similarities to glandular ones such as the balloon-like apical cells with numerous phenolics. At later developmental stages, and during secondary wall thickening, phenolics are transferred to the cell walls of the trichomes. Due to the diffuse deposition of phenolics in the cell walls, trichomes provide protection against UV-B radiation by behaving as optical filters, screening out wavelengths that could damage sensitive tissues. Protection from strong visible radiation is also afforded by increased surface light reflectance. Moreover, the mixtures of trichome phenolics represent a superficial chemical barrier that provides protection against biotic stress factors such as herbivores and pathogens. Although the cells of some trichomes die at maturity, they can modulate their quantitative and qualitative characteristics during development, depending on the prevailing conditions of the external biotic or abiotic environment. In fact, the structure and chemical constituents of trichomes may change due to the particular light regime, herbivore damage, wounding, water stress, salinity and the presence of heavy metals. Hence, trichomes represent dynamic protective structures that may greatly affect the outcome of many plant–environment interactions.

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Section: Non-glandular Trichomes Contain Phenolics Associated With Cell Wallsmentioning

confidence: 99%

Protective and defensive roles of non-glandular trichomes against multiple stresses: structure–function coordination

et al. 2019

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“…Olive transcriptomics, proteomics, and metabolomics [9][10][11][12] have begun to provide indications of candidate genes that may be key to the definition of an interesting phenotype. However, data from these high throughput approaches only has physiological value if the functionality is confirmed, usually by knockout or overexpression of target genes [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Somatic Embryogenesis from Mature Embryos of Olea europaea L. cv. ‘Galega Vulgar’ and Long-Term Management of Calli Morphogenic Capacity

Pires

Cardoso

Ribeiro

et al. 2020

Plants

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Several olive cultivars, characterized by high-quality olive oil show agronomical issues such as excessive vigor, high susceptibility to biotic and abiotic stresses, and low propagation ability. They are strong candidates for breeding based on new technologies to improve their performance in a short period of time. For this reason, the first step is developing efficient somatic embryogenesis (SE) protocols. Somatic embryogenesis in olive is highly genotype-dependent for both adult tissues and mature embryos as initial explants, requiring the development of specific protocols for each genotype. Trials using cotyledons and radicles as initial explants, isolated from ripe seeds from the Portuguese olive cv. ‘Galega vulgar’, gave more than 95% calli development. Radicles proved to be the most responsive tissue for SE induction, with an average of 2 embryos per callus after callus transfer to expression medium, and 14 embryos per callus after subculture on the olive cyclic embryogenesis medium (ECO). Embryogenic competence could be recovered after several subcultures on ECO medium that maintained cyclic embryogenesis for an indeterminate period of time. Embryo conversion and plant acclimatization were also attained with high success rates. Media management for cyclic embryogenesis maintenance is of general importance for SE protocols in any olive genotype. Somatic embryogenesis was thus attained for the first time in embryo-derived explants of cv. ‘Galega vulgar’.

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“…S1; Xiong et al ., ; Yuan et al ., ,,). This implies that EgrTBL16 may be novel SCW xylan‐modification gene or it may be involved in the acetylation of SCW glucomannan‐like TBL25 (Gille et al ., ; Haghighat et al ., ; Roka et al ., ; Zhong et al ., ). However, further functional validation needs to be completed to validate the association of this TBL with the biosynthesis or modification of an SCW polysaccharide.…”

Section: Resultsmentioning

confidence: 99%

A systems genetics analysis in Eucalyptus reveals coordination of metabolic pathways associated with xylan modification in wood‐forming tissues

et al. 2019

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Summary Acetyl‐ and methylglucuronic acid decorations of xylan, the dominant hemicellulose in secondary cell walls (SCWs) of woody dicots, affect its interaction with cellulose and lignin to determine SCW structure and extractability. Genes and pathways involved in these modifications may be targets for genetic engineering; however, little is known about the regulation of xylan modifications in woody plants. To address this, we assessed genetic and gene expression variation associated with xylan modification in developing xylem of Eucalyptus grandis × Eucalyptus urophylla interspecific hybrids. Expression quantitative trait locus (eQTL) mapping identified potential regulatory polymorphisms affecting gene expression modules associated with xylan modification. We identified 14 putative xylan modification genes that are members of five expression modules sharing seven trans‐eQTL hotspots. The xylan modification genes are prevalent in two expression modules. The first comprises nucleotide sugar interconversion pathways supplying the essential precursors for cellulose and xylan biosynthesis. The second contains genes responsible for phenylalanine biosynthesis and S‐adenosylmethionine biosynthesis required for glucuronic acid and monolignol methylation. Co‐expression and co‐regulation analyses also identified four metabolic sources of acetyl coenxyme A that appear to be transcriptionally coordinated with xylan modification. Our systems genetics analysis may provide new avenues for metabolic engineering to alter wood SCW biology for enhanced biomass processability.

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Proteome of olive non-glandular trichomes reveals protective protein network against (a)biotic challenge

Cited by 17 publications

References 72 publications

Protective and defensive roles of non-glandular trichomes against multiple stresses: structure–function coordination

Protective and defensive roles of non-glandular trichomes against multiple stresses: structure–function coordination

Somatic Embryogenesis from Mature Embryos of Olea europaea L. cv. ‘Galega Vulgar’ and Long-Term Management of Calli Morphogenic Capacity

A systems genetics analysis in Eucalyptus reveals coordination of metabolic pathways associated with xylan modification in wood‐forming tissues

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