Proteome of human plasma very low-density lipoprotein and low-density lipoprotein exhibits a link with coagulation and lipid metabolism

Dashti, Monireh; Levels, Johannes H.M.; Motazacker, Mohammad Mahdi; Vries, Margriet M. de; Mahmoudi, Mohammad Jafar; Peppelenbosch, Maikel P.; Rezaee, Farhad

doi:10.1016/j.atherosclerosis.2014.05.418

Cited by 11 publications

(20 citation statements)

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“…The mechanisms that underlie our observations are not yet known. A possible explanation is that the presence of C3 on the surface of lipoproteins [6][7][8] may affect their metabolism. This idea is supported by the observation that complement C1r, a component of the classical complement pathway, was indeed in vitro shown to bind phospholipid transfer protein and to partially stabilize its lipid transfer function.…”

Section: Discussionmentioning

confidence: 99%

“…C4 and C9 are present on very-low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL). [6][7][8] This suggests a possible relationship between the complement system and metabolism and/or function of circulating lipoproteins. Experimental findings also suggest that the complement system, particularly the alternative pathway, may be causally involved in lipid metabolism.…”

Section: Introductionmentioning

confidence: 99%

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C3 and alternative pathway components are associated with an adverse lipoprotein subclass profile: The CODAM study

Xin

Hertle

Kallen

et al. 2021

Journal of Clinical Lipidology

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BACKGROUND: Plasma lipoproteins contain heterogeneous subclasses. Previous studies on the associations of the complement system with lipids and lipoproteins are mainly limited to the major lipid classes, and associations of complement with lipoprotein subclass characteristics remain unknown. OBJECTIVE: We investigated the associations of C3 and other components of the alternative complement pathway with plasma lipoprotein subclass profile. METHODS: Plasma complement concentrations (complement component 3 [C3], properdin, factor H, factor D, MASP-3, C3a, Bb), and lipoprotein subclass profile (as measured by nuclear magnetic resonance spectroscopy) were obtained in 523 participants (59.6 6 6.9 years, 60.8% men) of the Cohort on Diabetes and Atherosclerosis Maastricht (CODAM) study. Multiple linear regression was used to investigate the associations of C3 (primary determinant) and other alternative pathway components (secondary determinants) with characteristics (particle concentration and size [main outcomes], and lipid contents [secondary outcomes]) of 14 lipoprotein subclasses, ranging from extremely large VLDL to small HDL (all standardized [std] values). RESULTS: Participants with higher C3 concentrations had more circulating VLDL (stdbs ranging from 0.27 to 0.36), IDL and LDL (stdbs ranging from 0.14 to 0.17), and small HDL (stdb 5 0.21). In contrast, they had fewer very large and large HDL particles (stdbs 5 20.36). In persons with higher C3 concentrations, all lipoprotein subclasses were enriched in triglycerides. Similar but weaker associations were observed for properdin, factor H, factor D, and MASP-3, but not for C3a and Bb.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

C3 and alternative pathway components are associated with an adverse lipoprotein subclass profile: The CODAM study

Xin

Hertle

Kallen

et al. 2021

Journal of Clinical Lipidology

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“… 15 Several proteins were reported as specific markers for a variety of lipoprotein subtypes, such as ApoA1, ApoB48 and ApoB100. 17 , 20 , 42 ApoA1 is majorly associated with HDL, and previous researches have shown SEC can remove >95% HDL. 19 , 31 Therefore, in this study, we used ApoB expression as the metrics to evaluate the lipoprotein level, which was also utilised by previous studies.…”

Section: Discussionmentioning

confidence: 96%

<p>Quality Assessment and Comparison of Plasma-Derived Extracellular Vesicles Separated by Three Commercial Kits for Prostate Cancer Diagnosis</p>

Pang

Zhu

et al. 2020

IJN

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Introduction Current standard biomarkers in clinic are not specific enough for prostate cancer (PCa) diagnosis. Extracellular vesicles (EVs) are nano-scale vesicles released by most mammalian cells. EVs are promising biomarker source for PCa liquid biopsy due to its minimal invasive approach, rich information and improved accuracy compared to the clinical standard prostate-specific antigen (PSA). However, current EV separation methods cannot separate pure EVs and the quality characteristics from these methods remain largely unknown. In this study, we evaluated the quality characteristics of human plasma-derived EVs by comparing three clinical suitable separation kits. Methods We combined EV separation by commercial kits with magnetic beads capture and flow cytometry analysis, and compared three kits including ExoQuick Ultra based on precipitation and qEV35 and qEV70 based on size exclusion chromatography (SEC). Results Our results indicated that two SEC kits provided higher EV purity and lower protein contamination compared to ExoQuick Ultra precipitation and that qEV35 demonstrated a higher EV yield but lower EV purity compared to qEV70. Particle number correlated very well particularly with CD9/81/63 positive EVs for all three kits, which confirms that particle number can be used as the estimate for EV amount. At last, we found that several EV metrics including total EVs and PSA-specific EVs could not differentiate PCa patients from health controls. Conclusion We provided a systematic workflow for the comparison of three separation kits as well as a general analysis process in clinical laboratories for EV-based cancer diagnosis. Better EV-associated cancer biomarkers need to be explored in the future study with a larger cohort.

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“…LDL cholesterol particles are referred to as bad cholesterol because they can transport their contents of lipid molecules into arterial walls attract macrophages, all of which result in atherosclerosis. Thus, increasing concentrations of LDL cholesterol particles are strongly associated with increasing rates of accumulation of atherosclerosis within the walls of arteries which over time, eventually results in sudden plaque ruptures, triggering clots within the artery opening (Dashty et al., ). The findings of this study with respect to the LDL cholesterol‐raising properties of boiled T. africana seed therefore call for caution in the consumption of this plant as a regular meal.…”

Section: Discussionmentioning

confidence: 99%

Effect of ethanol extract of boiled breadfruit (Treculia Africana) seed on the oral glucose tolerance, lipid profile, and body weight of normoglycemic albino rats

Eleazu

Maduabuchi

Eleazu

2018

Food Science & Nutrition

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The effect of ethanol extracts of boiled Treculia africana seed on the oral glucose tolerance (OGTT), lipid profile, and body weight of normoglycemic albino rats was determined. Fifteen male albino rats were used and were divided into three groups of five rats each. Groups 1 and 2 received 100 and 200 mg/kg of the extract, while group 3 (control group) received 1 ml/kg of normal saline. The experiment lasted for 28 days. The body weights of the rats were determined daily. OGTT was determined at week zero (before extract administration) and at weeks 2 and 4, respectively, following extract administration. Glycemic index (GI) of the extracts was calculated from the incremental area under the OGTT curve. The total cholesterol, high‐density lipoprotein (HDL) cholesterol, low‐density lipoprotein (LDL) cholesterol, and very‐low‐density lipoprotein (VLDL) cholesterol levels in the sera of the rats were determined using standard techniques. Atherogenic index (AI) and coronary risk index (CRI) of the rats were expressed as ratios of LDL cholesterol to HDL cholesterol and total cholesterol to HDL cholesterol, respectively. Following 2 weeks of the extract administration, the blood glucose for groups 1 and 2 rats declined to values ≤100 mg/dl after oral glucose loading. GI for the standard rat feeds and the extracts at 100 and 200 mg/kg by the second week of experimentation were 100, 114, and 96.09, respectively. GI for the extract at 100 mg/kg decreased to 103.63 at the 4th week, while that for the extract at 200 mg/kg increased to 98.07. The extract at 100 mg/kg increased the LDL cholesterol, AI, and CRI of the rats, suggesting that consumption of boiled African breadfruit may expose an individual to the risk of development of cardiovascular diseases. Finally, the study suggested that consumption of T. africana seed by a nondiabetic subject may have no effect on the glucose tolerance of the individual, while it will negatively impact on the glycemic status of a diabetic subject.

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Proteome of human plasma very low-density lipoprotein and low-density lipoprotein exhibits a link with coagulation and lipid metabolism

Cited by 11 publications

References 0 publications

C3 and alternative pathway components are associated with an adverse lipoprotein subclass profile: The CODAM study

C3 and alternative pathway components are associated with an adverse lipoprotein subclass profile: The CODAM study

<p>Quality Assessment and Comparison of Plasma-Derived Extracellular Vesicles Separated by Three Commercial Kits for Prostate Cancer Diagnosis</p>

Effect of ethanol extract of boiled breadfruit (Treculia Africana) seed on the oral glucose tolerance, lipid profile, and body weight of normoglycemic albino rats

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