Proteome of Agave angustifolia Haw.: Uncovering metabolic alterations, over-accumulation of amino acids, and compensatory pathways in chloroplast-deficient albino plantlets

Transcriptome analysis reveals molecular mechanisms underlying chloroplast biogenesis in albino Agave angustifolia plantlets

“…sharing an identical genetic background but displaying distinct phenotypes were generated via the micropropagation technique, as previously documented (Duarte‐Aké et al, 2016; Us‐Camas et al, 2017). The micropropagation and incubation protocols for both the green (G) and albino (A) plantlets (Figure 1) closely adhered to those reported by Andrade‐Marcial et al (2023). Leaf and shoot meristem (referred to as “meristem” throughout the article), from ~2.5‐cm tall plantlets of each phenotype, were used in this study, leading to the inclusion of four distinct study tissues: green leaf (GL), albino leaf (AL), green meristem (GM), and albino meristem (AM).…”

“…In an effort to elucidate the relationship between gene expression and protein accumulation during chloroplast biogenesis, a comparative analysis of transcriptomic and proteomic data derived was conducted for two somaclonal variants. The dataset regarding the differential accumulation of proteins in the two somaclonal variants under investigation was obtained from our laboratory and published by Andrade‐Marcial et al (2023). Differentially accumulated proteins (DAPs) were selected using a p ‐value ≤0.05 and a LFC of ≥0.58 denoted up‐accumulated proteins, while an LFC of ≤ −0.58 identified under‐accumulated proteins.…”

“…Our research indicates that variations in DNA methylation patterns during subculturing may contribute to the development of the albino condition in these plants. Despite advancements in delineating the structural and physiological anomalies of albino plantlets (Duarte‐Aké et al, 2016; Us‐Camas et al, 2017; Hernández‐Castellano et al, 2020; Duarte‐Aké & De‐la‐Peña, 2021; Andrade‐Marcial et al, 2022; Andrade‐Marcial et al, 2023), the primary causes at the genetic and molecular levels, particularly those influencing the signaling pathways of chloroplast biogenesis, remain to be fully elucidated.…”