Proteome modifications of Pacific white shrimp (Litopenaeus vannamei) muscle under biofloc system

Abstract

The objective of the study was to examine the effects of biofloc technology on the muscle proteome of Litopenaeus vannamei. Two biofloc treatments and one control were compared: biofloc‐based tanks under zero‐water exchange fed with 150 g/kg crude protein (BF15), or with 250 g/kg crude protein (BF25) diets, and clear water tanks with 50% of daily water exchange stocked with shrimp fed with similar amount of a 250 g/kg crude protein diet, referred to as control. The shrimp (5.28 ± 0.42 g) were divided into the 300‐L fibreglass tanks (water volume of 200 L) at a density of 35 shrimp per tank and were cultured for 35 days. The biofloc groups displayed better growth and survival compared to the control. The muscle tissue from the control and BF25 groups was subjected to proteomic analysis. Lactate dehydrogenase, enolase, arginine kinase, mitochondrial ATP synthase subunit alpha, mitochondrial ATPase inhibitor factor 1 precursor, serpin 3 and myeloid differentiation factor 88 had an increased abundance in the BF25 group, while myosin heavy chain type 1 and myosin heavy chain type 2 showed a decreased abundance. The results indicate that biofloc technology could alter the expression of proteins involved in structure, metabolism and immune status of cultured shrimp.

Research Article: The effect of biofloc-supplemented diets on the Pacific white shrimp (Litopenaeus vannamei): Analysis of water quality, growth performance, and biochemical composition

“…In this study, to change the efficiency of inorganic nitrogen to microbial protein, the C: N ratio was kept at 15: 1 (Avnimelech, 1999). The consumption of biofloc by shrimp has verified various merits, including the decline of food conversion ratio (Burford et al, 2004), the growth rate improvement (Wasielesky et al, 2006;Abbaszadeh et al, 2019). At the end of our experiment, in biofloc W10 treatment, the growth performance parameters were significantly higher than the control treatment.…”

“…It can also be used as a microbial protein source in the digestive system of fish and shrimp (Ekasari et al, 2014). There are bioactive compounds such as free amino acids, essential fatty acids, chlorophylls, minerals, and vitamins in the biofloc (Ju et al, 2008;Tacon et al, 2002;Crab et al, 2012) that can improve the immune system, antioxidant status, growth, and reproduction in aquatic animals (Ekasari et al, 2014;Abbaszadeh et al, 2019;Adineh et al, 2019).…”

Effect of Stocking Density and Dietary Protein Level in Biofloc System on the Growth, Digestive and Antioxidant Enzyme Activities, Health, and Resistance to Acute Crowding Stress in Juvenile Common Carp (Cyprinus carpio)

“…On day 60, the lowest TAN levels were observed in the control and MD biofloc groups. Previous studies also showed that TAN concentration was effectively reduced in the BFT groups [ 8 , 35 ]. Moreover, the lowest nitrite concentrations were observed in the MD25 (1.09 mg L −1 ) and control groups on day 50.…”

Water quality monitoring in recirculating aquaculture systems

“…In Biofloc system, microbial protein is produced for the raised species which improves feed conversion ratio, FCR (Khanjani & Sharifinia, 2020). Biofloc has been widely studied in recent decades for example in shrimp and finfish farming (Abbaszadeh et al., 2019; Ebrahimi et al., 2020).…”