Proteome-Level Investigation of Brassica carinata-Derived Resistance to Leptosphaeria maculans

Abstract

Plants resistant to the fungal pathogen Leptosphaeria maculans were generated by an interspecific cross between the highly susceptible Brassica napus (canola) and the highly resistant Brassica carinata. Changes in the leaf protein profiles of these lines were investigated in order to understand the biochemical basis for the observed resistance. Two-dimensional electrophoresis followed by tandem mass spectrometry led to the identification of proteins unique to the susceptible (5 proteins) and resistant genotypes (7 proteins) as well those that were differentially expressed in the resistant genotype 48 h after challenge with the pathogen (28 proteins). Proteins identified as being unique in the resistant plant material included superoxide dismutase, nitrate reductase, and carbonic anhydrase. Photosynthetic enzymes (fructose bisphosphate aldolase, triose phosphate isomerase, sedoheptulose bisphosphatase), dehydroascorbate reductase, peroxiredoxin, malate dehydrogenase, glutamine synthetase, N-glyceraldehyde-2-phosphotransferase, and peptidyl-prolyl cis-trans isomerase were observed to be elevated in the resistant genotype upon pathogen challenge. Increased levels of the antioxidant enzyme superoxide dismutase were further validated and supported by spectrophotometric and in-gel activity assays. Other proteins identified in this study such as nitrate reductase and peptidylprolyl isomerase have not been previously described in this plant-pathogen system, and their potential involvement in an incompatible interaction is discussed.

Changes of antioxidant enzymes of mung bean [Vigna radiata (L.) R. Wilczek] in response to host and non-host bacterial pathogens

“…Our data showed increment in expression of antioxidant enzymes in incompatible interaction versus compatible interaction that was in agreement with previous studies. Subramanian et al (2005) showed promotion in the levels of SOD in incompatible interaction between Brassica carinata -Leptosphaeria maculans compared to compatible interaction. Cheng et al (2012) displayed more expression of SOD and CAT in inoculated broad beans with a non-host pathogen compared to the control.…”

Comparative proteome analysis of metabolic changes by low phosphorus stress in two Brassica napus genotypes

“…Twodimensional gel electrophoresis (2-DE) is one of these tools, which allows the separation of several hundreds of gene products in a single gel and their subsequent quantification (Jorrín-Novo et al 2009). In B. napus, several studies on proteome analysis were conducted during seed filling (Hajduch et al 2006), in plastids of developing embryos (Jain et al 2008), and in pathogen-stress response against fungus Leptosphaeria maculans (Subramanian et al 2005) or against obligate parasite Plasmodiophora brassicae (Cao et al 2008) as well as comparative proteomics of leaf, stem, and root tissues (Albertin et al 2009). In other crops, this approach has been applied to study the plant response to phosphorus deficiency.…”

Media Optimization in Immobilized Culture to Enhance the Content of Curcumin in Curcuma longa (Zingiberaceae) and Protein Profile of Treated Samples in Static Culture

“…This may be of Glycine rich Protein (works on defence mechanism) which is already reported in Curcuma comosa [23]. There are some reports that support the hypothesis that biological damage mediated by the occurrence of ROS can be generated by abiotic stress [24][25][26]. The expression of protein (based on intensity of the bands) was reduced in treated samples as compared to control; this may be due to the formation of Reactive Oxygen species (ROS) in culture condition and which modified the protein.…”