Proteome Fishing for CRISPR/Cas12a-Based Orthogonal Multiplex Aptamer Sensing
Shuangqin Li,
Baichuan Jin,
Yintao Ma
et al.
Abstract:Detection of serum protein biomarkers is extremely challenging owing to the superior complexity of serum. Here, we report a method of proteome fishing from the serum. It uses a magnetic nanoparticle−protein corona and a multiplexed aptamer panel, which we incubated with the nanoparticle−protein corona for biomarker recognition. To transfer protein biomarker detection to aptamer detection, we established a CRISPR/Cas12a-based orthogonal multiplex aptamer sensing (COMPASS) platform by profiling the aptamers of p… Show more
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