Proteome characterization of a human urothelial cell line resistant to the bladder carcinogen 4-aminobiphenyl

Pastorelli, Roberta; Saletta, Federica; Carpi, Donatella; Campagna, Roberta; dell'Osta, Carlo; Schiarea, Silvia; Víneis, Paolo; Airoldi, Luisa; Matullo, Giuseppe

doi:10.1186/1477-5956-5-6

Cited by 6 publications

(6 citation statements)

References 20 publications

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“…Three different electrophoretic spots proved to be immunoreactive in the experimental window reported (pH 3–10, mass 10–200 kDa) showing approximately the same M r , but different pI values. The presence of protein variants differing in charge through amino acid differences or post-translational modifications is the most straightforward explanation of these shifts (Pastorelli et al, 2007). Representative mass spectra and the sequences of the assigned peptides for ANXA2 isoform 2 are shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Up-regulation of annexin A2 in cholangiocarcinoma caused by Opisthorchis viverrini and its implication as a prognostic marker

Yonglitthipagon

Pairojkul

Chamgramol

et al. 2010

International Journal for Parasitology

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Cholangiocarcinoma (CCA), or cancer of the bile ducts, is primarily associated with infection with the liver fluke Opisthorchis viverrini in northeast Thailand. The disease . is associated with late presentation, poses challenges for diagnosis and has a high mortality rate – features that highlight the need for tumor markers. At present, there are no specific tumor markers that can indicate the early stages and status of CCA. Proteomic analysis of the proteins expressed on the surface of tumor cells is particularly difficult since proteome-wide analysis of surface membrane proteins has thus far been hampered by the lack of effective strategies to profile hydrophobic membrane proteins. In this study, a sequential protein extraction was utilized to overcome this problem. Membrane protein was extracted from four CCA cell lines with different tumor forming capabilities. The non-tumor H69 biliary cell line was used as a control. Two-dimensional-PAGE followed by MALDI-TOF-MS was used to identify differentially expressed proteins. Among 20 up-regulated membrane proteins identified in the CCA cell lines was ANXA2, a participant in tumor invasion and metastasis in other cancers. Accordingly, ANXA2 was verified in human subjects by probing, using a commercial anti-mouse monoclonal antibody and a tissue microarray of CCA (301 diagnosed cases), where it was found to associate with one of several tumor progression stages as reflected by lymphatic invasion (P = 0.014) and metastasis (P = 0.026). Patients with high expression of ANXA2 had a significantly shorter survival time (P = 0.011). ANXA2 expression in tumors may be useful for predicting the poor outcome of CCA patients.

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Section: Resultsmentioning

confidence: 99%

Up-regulation of annexin A2 in cholangiocarcinoma caused by Opisthorchis viverrini and its implication as a prognostic marker

Yonglitthipagon

Pairojkul

Chamgramol

et al. 2010

International Journal for Parasitology

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“…The process was done as previously described (Pastorelli et al 2007). Briefly, total 30 lg proteins were separated by 10 % SDS-PAGE and transferred 1.5 h to a PVDF membrane (BioRad).…”

Section: Western Blottingmentioning

confidence: 99%

Identification of proteins differentially expressed in adriamycin-resistant (pumc-91/ADM) and parental (pumc-91) human bladder cancer cell lines by proteome analysis

Meng

Lei

Zhang

et al. 2012

J Cancer Res Clin Oncol

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“…It has been reported that ANNA1 is upregulated in human pancreatic cancer [20], breast cancer [21], hepatocellular carcinoma [22], and pituitary adenoma [23] but downregulated in human esophageal squamous cell carcinoma [24], prostate cancer [25], and B-cell nonHodgkin's lymphoma [26]. However, a recent study reported an overexpression of annexin 2 in bladder carcinoma RT112 sublines was related to assistance to aromatic amine 4-aminobiphenyl (4-ABP), a major etiological agent of human bladder cancer [27]. Consistent with this finding, we found that ANNA1 expression was decreased in human bladder cancer specimens compared to normal urothelial samples, although its significant overexpression was observed in rat bladder cancer induced by calculi.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of annexin a1 induced by terephthalic acid calculi in rat bladder cancer

et al. 2007

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Prolonged cell proliferation in response to irritation by bladder calculi can evoke malignant transformation of the urothelium. However, the molecular mechanisms responsible for calculi-associated bladder carcinogenesis are unknown. We compared the protein expression pattern of rat bladder transitional cell carcinomas (TCCs) induced by terephthalic acid with that of normal bladder tissues using 2-DE. Comparative analysis of the respective spot patterns on 2-DE showed 146 spots that were markedly changed in TCC samples. Subsequently, 56 of the variant protein spots were identified by MALDI-TOF MS. Among them, overexpression of annexin a1 (ANNA1) in rat TCCs was confirmed by Western blotting and real-time RT-PCR analysis. Immunohistochemical staining revealed that ANNA1, usually a cytoplasmic protein in normal urothelium, was translocated to the nucleus in rat bladder cancer cells. In contrast to the animal studies, examination of human clinical specimens showed that ANNA1 expression was reduced in TCC compared to normal urothelium. The expression of ANNA1 was inversely related to the level of differentiation of TCC. Our data suggest that overexpression of ANNA1 is involved in bladder carcinogenesis induced by bladder calculi and that translocation of the protein may be partly responsible for the effect. ANNA1 may serve as a new marker of differentiation for the histopathological grading of human TCC.

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Proteome characterization of a human urothelial cell line resistant to the bladder carcinogen 4-aminobiphenyl

Cited by 6 publications

References 20 publications

Up-regulation of annexin A2 in cholangiocarcinoma caused by Opisthorchis viverrini and its implication as a prognostic marker

Up-regulation of annexin A2 in cholangiocarcinoma caused by Opisthorchis viverrini and its implication as a prognostic marker

Identification of proteins differentially expressed in adriamycin-resistant (pumc-91/ADM) and parental (pumc-91) human bladder cancer cell lines by proteome analysis

Overexpression of annexin a1 induced by terephthalic acid calculi in rat bladder cancer

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