Proteome changes and associated physiological roles in chickpea (

Abstract

Interrogative proteome analyses are used to identify and quantify the expression of proteins involved in heat tolerance and to identify associated physiological processes in heat-stressed plants. The objectives of the study were to identify and quantify the expression of proteins involved in heat tolerance and to identify associated physiological processes in chickpea (Cicer arietinum L.) heat-tolerant (Acc#7) and sensitive genotype (Acc#8) from a field study. Proteomic and gene ontological analyses showed an upregulation in proteins related to protein synthesis, intracellular traffic, defence and transport in the heat-tolerant genotype compared to the susceptible one at the warmer site. Results from KEGG analyses indicate the involvement of probable sucrose-phosphate synthase (EC 2.4.1.14) and sucrose-phosphate phosphatase (EC 3.1.3.24) proteins, that were upregulated in the heat-tolerant genotype at the warmer site, in the starch and sucrose pathway. The presence of these differentially regulated proteins including HSP70, ribulose bisphosphate carboxylase/oxygenase activase, plastocyanin and protoporphyrinogen oxidase suggests their potential role in heat tolerance, at flowering growth stage, in field-grown chickpea. This observation supports unaltered physiological and biochemical performance of the heat-tolerant genotypes (Acc#7) relative to the susceptible genotype (Acc#8) in related studies (Makonya et al. 2019). Characterisation of the candidate proteins identified in the current study as well as their specific roles in the tolerance to heat stress in chickpea are integral to further crop improvement initiatives.

Genomic-Mediated Breeding Strategies for Global Warming in Chickpeas (Cicer arietinum L.)

“…Through this analysis, they uncovered several candidate genes associated with thermotolerance. Similarly, Makonya et al [221] investigated heat stress and found that the heat-tolerant genotypes exhibited an increase in specific proteins related to protein synthesis, defense, and transport during the flowering stage. These proteins included sucrose-phosphate synthase, sucrose-phosphatase, HSP70, ribulose bisphosphate carboxylase/oxygenase activase, plastocyanin, and protoporphyrinogen oxidase.…”

Temperature‐smart plants: A new horizon with omics‐driven plant breeding

“…In a study on Monterey pine ( Pinus radiata ) embryos exposed to two HS conditions (40°C for 4 h, 60°C for 5 min), proteomic analysis revealed the up‐regulation of ribosomes, cell wall carbohydrates, transmembrane transport proteins, HSPs and chaperones, post‐transcriptional regulation proteins, and fatty acid biosynthesis proteins and the down‐regulation of adenosylhomocysteinase protein, glycolytic pathway enzymes, nitrogen assimilation enzymes, oxidative stress enzymes, and methionine‐tRNA ligase (Castander‐Olarieta et al, 2021). Similarly, in chickpea ( Cicer arietinum L.), HS led to the differential expression of various proteins, including HSP70, ribulose bisphosphate carboxylase/oxygenase activase, plastocyanin oxidase and protoporphyrinogen oxidase, alongside the up‐regulation of proteins related to defense, transport, intracellular traffic, and protein biosynthesis (Makonya et al, 2021).…”

“…40 C for 4 h, 60 C for 5 min), proteomic analysis revealed the upregulation of ribosomes, cell wall carbohydrates, transmembrane transport proteins, HSPs and chaperones, post-transcriptional regulation proteins, and fatty acid biosynthesis proteins and the downregulation of adenosylhomocysteinase protein, glycolytic pathway enzymes, nitrogen assimilation enzymes, oxidative stress enzymes, and methionine-tRNA ligase(Castander-Olarieta et al, 2021). Similarly, in chickpea (Cicer arietinum L.), HS led to the differential expression of various proteins, including HSP70, ribulose bisphosphate carboxylase/oxygenase activase, plastocyanin oxidase and protoporphyrinogen oxidase, alongside the up-regulation of proteins related to defense, transport, intracellular traffic, and protein biosynthesis(Makonya et al, 2021).Wang et al (2021) reported 1,591 differentially abundant proteins (DAPs) related to stimuli response, structural molecule activity and transporter activity, catalytic activity, energy production and conversion, and carbohydrate transport and metabolism in two pepper varieties (heat-tolerant 17CL30 and heat-sensitive 05S180) upon HS exposure (40 C). In wheat under HS, Chunduri et al (2021) reported enhanced expression of DAPs involved in translation, gliadins, and low-molecular-weight glutenins and decreased expression of defenserelated, photosynthesis, glycolysis, and high-molecular-weight glutenins DAPs.…”

A Comprehensive Review on Chickpea (Cicer arietinum L.) Breeding for Abiotic Stress Tolerance and Climate Change Resilience

“…Regarding heat stress, Makonya et al [ 144 ] identified an up-regulation in proteins related to protein synthesis, intracellular traffic, defense, and transport in the heat-tolerant genotype (Acc#7). Among the proteins are sucrose-phosphate synthase, sucrose-phosphate phosphatase, HSP70, ribulose bisphosphate carboxylase/oxygenase activase, plastocyanin, and protoporphyrinogen oxidase, which have a role in heat tolerance at the flowering growth stage.…”