Proteome and immune responses of extracellular vesicles derived from macrophages infected with the periodontal pathogen <i>Tannerella forsythia</i>

Lim, Younggap; Kim, Hyun Young; Han, Dohyun; Choi, Bong‐Kyu

doi:10.1002/jev2.12381

Cited by 4 publications

(4 citation statements)

References 70 publications

(88 reference statements)

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“…Even amoxicillin was not statistically significant in reducing the amounts of this pathogen when compared to vehicle-treated biofilms. A recent article described that, during the infection of macrophages by T. forsythia, the immune response was led to produce pro-inflammatory factors (such as BspA, sialidase, GroEL, and numerous lipoproteins) related to periodontal tissue damage through the activation of the Toll-like 2 receptor of macrophages [31].…”

Section: Discussionmentioning

confidence: 99%

Combination of Neovestitol and Vestitol Modifies the Profile of Periodontitis-Related Subgingival Multispecies Biofilm

Macedo,

Malavazi,

Vargas

et al. 2024

Biomedicines

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The aim of this study was to evaluate the effect of the combination of neovestitol–vestitol (CNV) compounds obtained from Brazilian red propolis on the microbiological profile of a mature multispecies subgingival biofilm. The biofilm with 32 bacterial species associated with periodontitis was formed for seven days using a Calgary device. Treatment with CNV (1600, 800, 400, and 200 μg/mL), amoxicillin (54 μg/mL), and vehicle control was performed for 24 h on the last day of biofilm formation. Biofilm metabolic activity and DNA–DNA hybridization (checkerboard) assays were performed. The groups treated with CNV 1600 and amoxicillin reduced 25 and 13 species, respectively, compared to the control vehicle treatment (p ≤ 0.05); both reduced P. gingivalis, while only CNV reduced T. forsythia. When the data from the two treatments (CNV and AMOXI) were compared, a statistically significant difference was observed in 13 species, particularly members of Socransky’s orange complex. Our results showed that CNV at 1600 μg/mL showed the best results regarding the metabolic activity of mature biofilms and obtained a reduction in species associated with the disease, such as T. forsythia, showing a better reduction than amoxicillin. Therefore, CNV seems to be a promising alternative to eradicate biofilms and reduce their pathogenicity.

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Section: Discussionmentioning

confidence: 99%

Combination of Neovestitol and Vestitol Modifies the Profile of Periodontitis-Related Subgingival Multispecies Biofilm

Macedo,

Malavazi,

Vargas

et al. 2024

Biomedicines

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“…Remarkably, the prevalence of the T. forsythia BspA genotype is markedly elevated in patients with chronic and aggressive periodontitis, suggesting a direct contribution of BspA to periodontal disease (44). Additionally, T. forsythia and BspA induce foam cell formation in THP-1 macrophages and promote the progression of atherosclerotic lesions in ApoE (−/−) mice (45), indicating a potential impact of this virulence factor on systemic health.…”

Section: Cell Surface Antigen Bspamentioning

confidence: 99%

“…Outer membrane vesicles (OMVs) from T. forsythia induce the production of TNF-α and IL-8 in U937 macrophages, and IL-6, IL-8, and MCP-1 in human periodontal ligament mesenchymal stromal cells (hPDL-MSCs) in a concentration-dependent manner ( 46 ). The response of host cells to T. forsythia OMVs is comparable to or even stronger than that elicited by the whole bacterium ( 46 ) and is mediated through TLR2 activation ( 47 ). Monocytes and differentiated macrophages may phagocytose T. forsythia's OMVs, leading to the activation of a pro-inflammatory response ( 48 ).…”

Section: Virulence Factors Of T Forsythiamentioning

confidence: 99%

The intriguing strategies of Tannerella forsythia's host interaction

Schäffer,

Andrukhov

2024

Front. Oral. Health

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Tannerella forsythia, a member of the “red complex” bacteria implicated in severe periodontitis, employs various survival strategies and virulence factors to interact with the host. It thrives as a late colonizer in the oral biofilm, relying on its unique adaptation mechanisms for persistence. Essential to its survival are the type 9 protein secretion system and O-glycosylation of proteins, crucial for host interaction and immune evasion. Virulence factors of T. forsythia, including sialidase and proteases, facilitate its pathogenicity by degrading host glycoproteins and proteins, respectively. Moreover, cell surface glycoproteins like the S-layer and BspA modulate host responses and bacterial adherence, influencing colonization and tissue invasion. Outer membrane vesicles and lipopolysaccharides further induce inflammatory responses, contributing to periodontal tissue destruction. Interactions with specific host cell types, including epithelial cells, polymorphonuclear leukocytes macrophages, and mesenchymal stromal cells, highlight the multifaceted nature of T. forsythia's pathogenicity. Notably, it can invade epithelial cells and impair PMN function, promoting dysregulated inflammation and bacterial survival. Comparative studies with periodontitis-associated Porphyromonas gingivalis reveal differences in protease activity and immune modulation, suggesting distinct roles in disease progression. T. forsythia's potential to influence oral antimicrobial defense through protease-mediated degradation and interactions with other bacteria underscores its significance in periodontal disease pathogenesis. However, understanding T. forsythia's precise role in host-microbiome interactions and its classification as a keystone pathogen requires further investigation. Challenges in translating research data stem from the complexity of the oral microbiome and biofilm dynamics, necessitating comprehensive studies to elucidate its clinical relevance and therapeutic implications in periodontitis management.

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“…Moreover, it is estimated that severe periodontitis leads to an annual global loss in productivity of 54 billion dollars [4]. Furthermore, periodontitis is strongly associated with various systemic diseases, such as Alzheimer's disease, adverse pregnancy outcomes, rheumatoid arthritis, cardiovascular disease, and diabetes [5][6][7]. Consequently, preventing the occurrence of periodontitis is crucial not only to oral health but also to systemic health.…”

Section: Introductionmentioning

confidence: 99%

The association between polyunsaturated fatty acids and periodontitis: NHANES 2011–2014 and Mendelian randomisation analysis

Li,

Wu,

et al. 2024

Lipids Health Dis

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Background We aimed to explore the association and potential causality between polyunsaturated fatty acids concentrations and the risk of periodontal disease. Materials and methods Data were collected from the 2011–2014 National Health and Nutrition Examination Survey (NHANES). Weighted logistic regression analysis and restricted cubic spline (RCS) analysis were used to analyse the associations of the concentrations of omega-3 and omega-6 fatty acids and the omega-6/omega-3 fatty acids ratio with the risk of periodontitis. E-value and propensity score matching (PSM) analyses were used for sensitivity analyses. In addition, two-sample Mendelian randomisation (MR) analyses were performed to assess the potential causal impact of the concentrations of those fatty acids on periodontitis risk. Results A total of 2462 participants from the NHANES were included. Logistic regression analysis revealed that high omega-3 fatty acids levels were negatively associated with the risk of developing periodontitis (P < 0.05), while the omega-6/omega-3 fatty acids ratio was positively associated with the risk of developing periodontitis (P < 0.05). There was no significant association between omega-6 concentrations and the risk of periodontitis. The findings mentioned above were confirmed by analysis following a 1:1 PSM. Furthermore, MR examination of the two samples indicated no possible causal link between the risk of periodontitis and the concentrations of omega-3 or omega-6 fatty acids or the ratio of omega-6 to omega-3 fatty acids (P > 0.05). Conclusion Although omega-3 fatty acids and the omega-6/omega-3 fatty acids ratio were associated with the risk of periodontitis in cross-sectional studies, the MR results did not support a causal relationship between them. Therefore, there is no indication that an increase in the omega-3 fatty acids concentration or a decrease in the omega-6/omega-3 fatty acids ratio may be beneficial for preventing periodontitis.

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Proteome and immune responses of extracellular vesicles derived from macrophages infected with the periodontal pathogen Tannerella forsythia

Cited by 4 publications

References 70 publications

Combination of Neovestitol and Vestitol Modifies the Profile of Periodontitis-Related Subgingival Multispecies Biofilm

Combination of Neovestitol and Vestitol Modifies the Profile of Periodontitis-Related Subgingival Multispecies Biofilm

The intriguing strategies of Tannerella forsythia's host interaction

The association between polyunsaturated fatty acids and periodontitis: NHANES 2011–2014 and Mendelian randomisation analysis

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