Proteome analysis of the causative agent of Chagas disease: Trypanosoma cruzi

Parodi-Talice, Adriana; Durán, Rosario; Arrambide, Nicolás; Prieto, Victoria; Piñeyro, María Dolores; Pritsch, Otto; Cayota, Alfonso; Červeñanský, Carlos; Robello, Carlos

doi:10.1016/j.ijpara.2004.05.002

Cited by 61 publications

(56 citation statements)

References 18 publications

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“…mass spectrometry (MS)]. Contrary to previous preliminary studies (27), the present work relies on a firm phylogenetic framework to explore T. cruzi proteomic diversity.…”

mentioning

confidence: 99%

Phylogenetic character mapping of proteomic diversity shows high correlation with subspecific phylogenetic diversity in Trypanosoma cruzi

Tellería

Biron²,

Brizard

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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We performed a phylogenetic character mapping on 26 stocks of Trypanosoma cruzi, the parasite responsible for Chagas disease, and 2 stocks of the sister taxon T. cruzi marinkellei to test for possible associations between T. cruzi-subspecific phylogenetic diversity and levels of protein expression, as examined by proteomic analysis and mass spectrometry. We observed a high level of correlation (P < 10) between genetic distance, as established by multilocus enzyme electrophoresis, and proteomic dissimilarities estimated by proteomic Euclidian distances. Several proteins were found to be specifically associated to T. cruzi phylogenetic subdivisions (discrete typing units). This study explores the previously uncharacterized links between infraspecific phylogenetic diversity and gene expression in a human pathogen. It opens the way to searching for new vaccine and drug targets and for identification of specific biomarkers at the subspecific level of pathogens.infectious disease | parallel evolution | gene expression | Chagas disease | parasite

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“…mass spectrometry (MS)]. Contrary to previous preliminary studies (27), the present work relies on a firm phylogenetic framework to explore T. cruzi proteomic diversity.…”

mentioning

confidence: 99%

Phylogenetic character mapping of proteomic diversity shows high correlation with subspecific phylogenetic diversity in Trypanosoma cruzi

Tellería

Biron²,

Brizard

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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“…Among these uncommon characteristics are the organization of the open reading frames into long polycistronic regions and post-transcriptional processing that makes the regulation of gene expression in these protozoa peculiar (Clayton and Shapira 2007 ). The regulation depends on the stability and translation of each mRNA, and the protein content is directly related to this post-translational control (Parodi-Talice et al 2004 ;Andrade et al 2008 ). More recently, the presence of non-translated mRNA was detected in the T. cruzi cytosol (Holetz et al 2010 ), reinforcing the notion that RNA-based techniques may be limited for the evaluation of parasite gene expression.…”

Section: State Of the Artmentioning

confidence: 99%

The Expected Outcome of the Trypanosoma cruzi Proteomic Map: A Review of Its Potential Biological Applications for Drug Target Discovery

Menna-Barreto

Perales

2013

Subcellular Biochemistry

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Chagas disease is a neglected tropical illness endemic to Latin America, and its treatment remains unsatisfactory. This disease is caused by the hemoflagellate protozoan Trypanosoma cruzi, which has a complex life cycle involving three evolutive forms in both vertebrate and invertebrate hosts. Targeting metabolic pathways in the parasite for rational drug design represents a promising research field. This research area requires high performance techniques and proteomics become a powerful tool in this context. Here, we review advances in the construction of proteomic maps of the different forms of T. cruzi, emphasizing their biological applications towards the identification of alternative candidates for drug intervention.

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“…, Nirmalan, et al, 2004, Trypanosoma sp. (Paba, et al, 2004, Parodi-Talice, et al, 2004, van Deursen, et al, 2003, Leishmania sp. (Acestor, et al, 2002, Drummelsmith, et al, 2003, El Fakhry, et al, 2002, Nugent, et al, 2004, Thiel and Bruchhaus, 2001, Toxoplasma gondii and Cryptosporidium sp.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Proteomic analysis of Giardia: Studies from the pre- and post-genomic era

Steuart¹

2010

Experimental Parasitology

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The proteome of Giardia duodenalis has been under study for the last 25 years and has lead to the discovery of valuable information on the biology and variation of the parasite.Proteomic techniques, mainly SDS-PAGE and 2D-PAGE, have been used to investigate protein variation, cellular structure and host parasite interactions. This has allowed for the identification of assemblage and host specific proteins, structural proteins, proteins released by trophozoites upon exposure to host cell monolayers and immunoreactive proteins. These data are important in understanding the pathogenesis of G. duodenalis infections, as well as highlighting potential drug and vaccine targets. There is however a large amount of future work needed to fully understand the proteome of this parasite.

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Proteome analysis of the causative agent of Chagas disease: Trypanosoma cruzi

Cited by 61 publications

References 18 publications

Phylogenetic character mapping of proteomic diversity shows high correlation with subspecific phylogenetic diversity in Trypanosoma cruzi

Phylogenetic character mapping of proteomic diversity shows high correlation with subspecific phylogenetic diversity in Trypanosoma cruzi

The Expected Outcome of the Trypanosoma cruzi Proteomic Map: A Review of Its Potential Biological Applications for Drug Target Discovery

Proteomic analysis of Giardia: Studies from the pre- and post-genomic era

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