Proteome analysis of soybean roots subjected to short-term drought stress

Abstract

Drought is one of the most important constraints on the growth and productivity of many crops, including soybeans. However, as a primary sensing organ, the plant root response to drought has not been well documented at the proteomic level. In the present study, we carried out a proteome analysis in combination with physiological analyses of soybean roots subjected to severe but recoverable drought stress at the seedling stage. Drought stress resulted in the increased accumulation of reactive oxygen species and subsequent lipid peroxidation. The proline content increased in drought-stressed plants and then decreased during the period of recovery. The high-resolution proteome map demonstrated significant variations in about 45 protein spots detected on Comassie briliant blue-stained 2-DE gels. Of these, 28 proteins were identified by mass spectrometry; the levels of 5 protein spots were increased, 21 were decreased and 2 spots were newly detected under drought condition. When the stress was terminated by watering the plants for 4 days, in most cases, the protein levels tended towards the control level. The proteins identified in this study are involved in a variety of cellular functions, including carbohydrate and nitrogen metabolism, cell wall modification, signal transduction, cell defense and programmed cell death, and they contribute to the molecular mechanism of drought tolerance in soybean plants. Analysis of protein expression patterns revealed that proteins associated with osmotic adjustment, defense signaling and programmed cell death play important roles for soybean plant drought adaptation. The identification of these proteins provides new insight that may lead to a better understanding of the molecular basis of the drought stress responses. Abbreviations 2-DE Two-dimensional gel electrophoresis MALDI-TOF Matrix-assisted laser desorption ionization time-of-flight PMF Peptide mass fingerprinting ROS Reactive oxygen species SDS-PAGE Sodium dodecylsulfate polyacrylamide gel electrophoresis TBARS Thiobarbituric acid reactive substance Plant Soil (2010) 333:491-505

Proteomics Approach for Identifying Abiotic Stress Responsive Proteins in Soybean

“…The Sadenosylmethionine synthetase gene is expressed in all living cells, and its product, Sadenosyl-L-methionine, is the major methyl donor in all cells. It has been shown that the expression of S-adenosylmethionine synthetase in soybean root decreases upon exposure to drought stress (Alam et al, 2010b). Down-regulation of this enzyme under drought is consistent with the inhibition of photosynthetic activity as a general feature of abiotic stresses.…”

“…Carbohydrate metabolism is likely to be most affected by drought stress after photosynthesis. Proteome analysis of soybean root under drought condition showed that two key enzymes involved in carbohydrate metabolism, UDPglucose pyrophosphorylase and 2,3-bisphosphoglycerate independent phosphoglycerate mutase, were down-regulated upon exposure to drought (Alam et al, 2010b). The levels of expression of both enzymes tended to revert to that of the control plants when watering was restored.…”

“…In the case of drought stress, up-regulation of reactive oxygen species (ROS) scavengers such as superoxide dismutase (SOD) was reported in soybean seedlings (Toorchi et al, 2009) and in rice (Ali & Komatsu, 2006). Proteomic investigations on soybean root revealed the accumulation of dehydrin and ferritin under drought stress (Alam et al, 2010b). Dehydrins, which belong to the group of late-embryogenesis-abundant (LEA) proteins, have been reported to function in abiotic stress tolerance by minimizing the negative effects of ROS (Mowla et al, 2006).…”

Tetracycline Accumulation in Pea Seedlings and its Effects on Proteome and Enzyme Activities

“…This protein is believed to have a detoxicating capability in case of excessive concentrations of iron, ozone, NO or hydrogen peroxide (Zhao et al, 2002;Theil et al, 2006;Arosio et al, 2009); it may also be able to detoxicate the cell from tetracycline. Data accumulated so far indicate that ferritin is stimulated by dehydration and excessive moisture content (Ravet et al, 2009;Alam et al, 2010).…”

Biochemical response of three Vigna mungo varieties (T9, RBU38 and VM4) under drought stress

“…After recovery, decreased proline content was observed but it remained higher for each variety when compared with the control plants of the same variety. Studies of plants under drought stress indicate proline accumulation as a protective behaviour (Alam et al, 2010;Keyvan, 2010;Yazdanpanah et al, 2011;Alam et al, 2013). Turkan et al (2005) observed higher proline content in drought tolerant plants in comparison to sensitive plants.…”