Proteome analysis of matrix vesicles isolated from femurs of chicken embryo

Balcerzak, Marcin; Malinowska, Agata; Thouverey, Cyril; Sekrecka, Anna; Dadlez, Michał; Buchet, René; Pikula, Sławomir

doi:10.1002/pmic.200700612

Cited by 87 publications

(91 citation statements)

References 90 publications

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“…These membrane proteins play a role in the efflux of metabolites including ATP and phosphate in the mitochondria. 40 Interestingly, these proteins are recurrently identified in matrix vesicles (MV), 14,41 the organelles implicated in initiation of mineralization. 41,43 Start of mineral deposition occurs by accommodating the proper environment for crystal growth in the MV.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Human Osteoblastic Cells: Relevant Proteins and Functional Categories for Differentiation

et al. 2010

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Osteoblasts are the bone forming cells, capable of secreting an extracellular matrix with mineralization potential. The exact mechanism by which osteoblasts differentiate and form a mineralized extracellular matrix is presently not fully understood. To increase our knowledge about this process, we conducted proteomics analysis in human immortalized preosteoblasts (SV-HFO) able to differentiate and mineralize. We identified 381 proteins expressed during the time course of osteoblast differentiation. Gene ontology analysis revealed an overrepresentation of protein categories established as important players for osteoblast differentiation, bone formation, and mineralization such as pyrophosphatases. Proteins involved in antigen presentation, energy metabolism and cytoskeleton rearrangement constitute other overrepresented processes, whose function, albeit interesting, is not fully understood in the context of osteoblast differentiation and bone formation. Correlation analysis, based on quantitative data, revealed a biphasic osteoblast differentiation, encompassing a premineralization and a mineralization period. Identified differentially expressed proteins between mineralized and nonmineralized cells include cytoskeleton (e.g., CCT2, PLEC1, and FLNA) and extracellular matrix constituents (FN1, ANXA2, and LGALS1) among others. FT-ICR-MS data obtained for FN1, ANXA2, and LMNA shows a specific regulation of these proteins during the different phases of osteoblast differentiation. Taken together, this study increases our understanding of the proteomics changes that accompany osteoblast differentiation and may permit the discovery of novel modulators of bone formation.

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Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Human Osteoblastic Cells: Relevant Proteins and Functional Categories for Differentiation

et al. 2010

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“…Nascent HA crystals exposed to the extravesicular fluid have been found to bind OPN, a collagen-binding molecule (Chen et al 1992;Liu et al 2007;Martin et al 2004). Given that MVs display collagen-binding molecules on their membranes (TNAP, Annexin A5 (AnxA5)) (Balcerzak et al 2008;Wu et al 1991;Xiao et al 2007), it is conceivable that a specific binding of MVs to collagen mediates the physical transfer of HA crystals to the exterior of collagen fibrils. This model does not negate, nor is it incompatible with, data indicating that crystals are able to form in the gaps and holes of the collagen fibrils, as has been well demonstrated by the work of many investigators, but if proven correct it would help explain how crystals formed within MVs can contribute to ECM mineralization.…”

Section: The Role Of Phosphatases In Mv-mediated Initiation Of Skeletmentioning

confidence: 99%

“…Almost 40 years after the existence of the NKA pump was proposed, Hsu and Anderson (1996) described that an ATPase inhibited by vanadate, but not by alkaline phosphatase, could be also responsible for initiating Ca 2+ and P i deposition into intact MVs (Hsu and Anderson 1996). In a study carried out much later on the proteome of the chicken embryo, Balcerzak et al (2008) revealed MVs to be a very complex biological environment where they identified 23 transporter proteins. Among them, five α and β subunits from NKA self-assemble in specific dimers that can subsequently associate into multimeric species (Tokhtaeva et al 2012;Yoneda et al 2016).…”

Section: The Role Of Transporters In Mv-mediated Initiation Of Skeletmentioning

confidence: 99%

“…Among the usual protein markers of lipid rafts domains (Foster et al 2003), AnxA6 and TNAP have been found in MVs isolated from epiphyseal growth plate cartilage (Balcerzak et al 2008), suggesting that the lipid composition is as important as the protein composition for the role of MVs in the mineralization process. Bolean et al (2010Bolean et al ( , 2011 therefore carried out experiments to better understand the role of the lipids present in lipid rafts and their interactions with TNAP by means of differential scanning calorimetry using the substrates DPPC, Chol, SM and the ganglioside GM1.…”

Section: What Are the Biomimetic Systems Of Mvs?mentioning

confidence: 99%

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Biophysical aspects of biomineralization

et al. 2017

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During the process of endochondral bone formation, chondrocytes and osteoblasts mineralize their extracellular matrix (ECM) by promoting the synthesis of hydroxyapatite (HA) seed crystals in the sheltered interior of membranelimited matrix vesicles (MVs). Several lipid and proteins present in the membrane of the MVs mediate the interactions of MVs with the ECM and regulate the initial mineral deposition and posterior propagation. Among the proteins of MV membranes, ion transporters control the availability of phosphate and calcium needed for initial HA deposition. Phosphatases (orphan phosphatase 1, ectonucleotide pyrophosphatase/ phosphodiesterase 1 and tissue-nonspecific alkaline phosphatase) play a crucial role in controlling the inorganic pyrophosphate/inorganic phosphate ratio that allows MVmediated initiation of mineralization. The lipidic microenvironment can help in the nucleation process of first crystals and also plays a crucial physiological role in the function of MVassociated enzymes and transporters (type III sodiumdependent phosphate transporters, annexins and Na + /K + ATPase). The whole process is mediated and regulated by the action of several molecules and steps, which make the process complex and highly regulated. Liposomes and proteoliposomes, as models of biological membranes, facilitate the understanding of lipid-protein interactions with emphasis on the properties of physicochemical and biochemical processes. In this review, we discuss the use of proteoliposomes as multiple protein carrier systems intended to mimic the various functions of MVs during the initiation and propagation of mineral growth in the course of biomineralization. We focus on studies applying biophysical tools to characterize the biomimetic models in order to gain an understanding of the importance of lipid-protein and lipid-lipid interfaces throughout the process.Keywords Lipid microenvironment . Biomineralization . Matrix vesicles . Liposome . Proteoliposome . Hydroxyapatite Mineralization process and the biogenesis of matrix vesiclesMineralization of cartilage and bone occurs by a series of physicochemical and biochemical processes that together facilitate the deposition of calcium phosphate. The deposited calcium phosphate is subsequently converted into hydroxyapatite (HA) [Ca 10 (PO 4 ) 6 (OH) 2 ] in specific areas of the extracellular matrix (ECM). Various experiments have revealed the presence of HA crystals both inside and outside collagen fibrils in the ECM (McNally et al. 2012) and also within the

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“…While progress has been made in the study of MVs, (3,(7)(8)(9)(10). Data on the MVs protein component have been generated.…”

Section: Introductionmentioning

confidence: 99%

MVsCarta: A protein database of matrix vesicles to aid understanding of biomineralization

Cui

Luan

et al. 2015

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Proteome analysis of matrix vesicles isolated from femurs of chicken embryo

Cited by 87 publications

References 90 publications

Proteomic Analysis of Human Osteoblastic Cells: Relevant Proteins and Functional Categories for Differentiation

Proteomic Analysis of Human Osteoblastic Cells: Relevant Proteins and Functional Categories for Differentiation

Biophysical aspects of biomineralization

MVsCarta: A protein database of matrix vesicles to aid understanding of biomineralization

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