Proteome analysis of Pueraria mirifica tubers collected in different seasons

Abstract

Pueraria mirifica-derived tuberous powder has been long-term consumed in Thailand as female hormone-replacement traditional remedies. The protein profiles of tubers collected in different seasons were evaluated. Phenol extraction, 2D-PAGE, and mass spectrometry were employed for tuberous proteome analysis. Out of the 322 proteins detected, over 59% were functionally classified as being involved in metabolism. The rest proteins were involved in defense, protein synthesis, cell structure, transportation, stress, storage, and also unidentified function. The proteins were found to be differentially expressed with respect to harvest season. Importantly, chalcone isomerase, isoflavone synthase, cytochrome p450, UDP-glycosyltransferase, and isoflavone reductase, which are all involved in the biosynthesis pathway of bioactive isoflavonoids, were most abundantly expressed in the summer-collected tubers. This is the first report on the proteomic patterns in P. mirifica tubers in relevant with seasonal variation. The study enlights the understanding of variance isoflavonoid production in P. mirifica tubers.

The Eucalyptus grandis chloroplast proteome: Seasonal variations in leaf development

“…In addition to its role in chlorophyll biosynthesis, PORA can also exert a photoprotective action in the transition from dark to light [ 67 ], whereas the Triose phosphate isomerase enzyme (TPI) interconverts glyceraldehyde 3-phosphate (G3P) in the Calvin cycle placed in chloroplasts. Up-regulation of TPI in the winter has been reported in experiments performed on tubers and Eucalyptus grandis bark [ 68 , 69 ].…”

The Eucalyptus grandis chloroplast proteome: leaf development and seasonal variations

“…In addition to its role in chlorophyll biosynthesis, PORA can also exert a photoprotective actionin the transition from dark to light [74], whereas the Triosephosphate isomerase enzyme (TPI) interconverts glyceraldehyde 3-phosphate (G3P) in the Calvin cycle placed in chloroplasts. Up-regulation of TPI in the winter has been reported in experiments performed on tubers and Eucalyptus grandis bark [75, 76].…”

Hormonal and transcriptional analyses provides new insights into the molecular mechanisms underlying root thickening and isoflavonoid biosynthesis in Callerya speciosa (Champ. ex Benth.) Schot

“…CHIs might regulate the efficient metabolic flux of isoflavonoid biosynthesis by interacting with CHS1, IFS1, and CYP93C in soybean 36 . It was reported that genes encoding CHI, CHS, CYP, and isoflavone reductase (IFR) were all involved in the biosynthesis pathway of bioactive isoflavonoids and most abundantly expressed in the summer-collected tubers in Pueraria mirifica 37 . Many isoflavonoids could also be O-methylated at hydroxyl groups by O-methyltransferases (OMTs), and 4′-O-methylated under the action of HI4′OMTs, which was reported to be important for isoflavonoids (e.g., formononetin) biosynthesis in several www.nature.com/scientificreports/ leguminous plant species 38 .…”

“…In addition, MYB44s, bHLH35, and WRKY41 were also reported to regulate the anthocyanin biosynthesis 46,51,52 , which might be involved in isoflavonoid biosynthesis. Genes encoding GBSS, SUS, PGM, UGP, and BAMY, were reported to be associated with sucrose hydrolyzation and starch synthesis during tuberous root thickening in several species 5,20 , while genes encoding CHI, CHS, HIDM, HI4′OMT, VR, IFS and I3′H were found to be involved in the isoflavonoid metabolism pathway 17,[35][36][37] . Taken together, the results indicated that all these DEGs potentially involved in the regulatory model of root thickening and isoflavonoid biosynthesis in C. speciosa.…”