Proteome Analysis of Human Aqueous Humor

Chowdhury, Uttio Roy; Madden, Benjamin J.; Charlesworth, Mary Christine; Fautsch, Michael P.

doi:10.1167/iovs.10-5531

Cited by 156 publications

(187 citation statements)

References 40 publications

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“…15 TTR is present in almost all ocular tissues in noninflammatory eyes, including AH. [16][17][18] TTR in the vitreous has been demonstrated not to originate from the blood. 17 Similarly, considering its suggested production by CPE, local production of TTR in AH seems plausible.…”

Section: Discussionmentioning

confidence: 99%

“…27 In the past, AH was considered to be a plasma filtrate, 27,28 however, recent studies show the presence of many specific proteins in normal AH. 16,18 Although the functions of the majority of these proteins are not known, their presence suggests active local production by cells and tissues of the anterior segment. Expression levels of AH proteins in our study showed significant correlation with each other, which could imply their interactions in the inflammatory or, alternatively, regulatory molecular mechanisms.…”

Section: Discussionmentioning

confidence: 99%

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Intraocular Biomarker Identification in Uveitis Associated With Juvenile Idiopathic Arthritis

Ayuso

Boer

Byers

et al. 2013

Invest. Ophthalmol. Vis. Sci.

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PURPOSE.To investigate the presence of biomarkers in aqueous humor (AH) from patients with uveitis associated with juvenile idiopathic arthritis (JIA).METHODS. AH (n ¼ 73) and serum (n ¼ 105) samples from 116 children were analyzed using surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-ToF MS). The samples were divided into the following groups: JIA, silent chronic anterior uveitis (AU), other uveitis entities, and noninflammatory controls. Statistical biomarker identification was performed using the SELDI-ToF Biomarker Analysis Cluster Wizard followed by multivariate statistical analysis. Biochemical identification of biomarkers was performed by polyacrylamide gel protein separation, followed by liquid chromatography tandem mass spectrometry. ELISA was performed in a number of AH samples representing all four study groups.RESULTS. In the JIA group, one AH protein peak at mass/charge (m/z) 13,762 had qualitative and quantitative differences in expression compared with the other uveitis entities and the controls, but not to the group of silent chronic AU. Its quantitative expression in AH of patients with JIA and other silent chronic AU was positively associated with uveitis activity. The protein at m/z 13,762 in AH was identified as transthyretin (TTR). The TTR concentration in AH differed significantly between the study groups (P ¼ 0.006) with considerably higher TTR concentrations in JIA and silent chronic AU samples positive for m/z 13,762 than those of the other uveitis and control groups.CONCLUSIONS. TTR is a potential intraocular biomarker of JIA-associated uveitis. Its role in the pathogenesis of silent chronic AU with and without arthritis needs further investigation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Intraocular Biomarker Identification in Uveitis Associated With Juvenile Idiopathic Arthritis

Ayuso

Boer

Byers

et al. 2013

Invest. Ophthalmol. Vis. Sci.

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“…It contains electrolytes, proteins, cytokines, organic solutes, and growth factors to nourish the avascular tissue of the anterior chamber. 4 Most proteins are released through active secretion. 5 Na + is released by epithelial cells of the ciliary body via membrane Na + /K + ATPases, and chloride reaches the aqueous humor via Cl -channels.…”

Section: Physiology Of Aqueous Humor Production and Outflowmentioning

confidence: 99%

Pharmacotherapy of Glaucoma

Schmidl

Schmetterer²,

Garhöfer³

et al. 2015

Journal of Ocular Pharmacology and Therapeutics

127

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Glaucoma is a group of diseases involving the optic nerve and associated structures, which is characterized by progressive visual field loss and typical changes of the optic nerve head (ONH). The only known treatment of the disease is reduction of intraocular pressure (IOP), which has been shown to reduce glaucoma progression in a variety of large-scale clinical trials. Nowadays, a relatively wide array of topical antiglaucoma drugs is available, including prostaglandin analogues, carbonic anhydrase inhibitors, beta-receptor antagonists, adrenergic agonists, and parasympathomimetics. In clinical routine, this allows for individualized treatment taking risk factors, efficacy, and safety into account. A major challenge is related to adherence to therapy. Sustained release devices may help minimize this problem but are not yet available for clinical routine use. Another hope arises from non-IOP-related treatment concepts. In recent years, much knowledge has been gained regarding the molecular mechanisms that underlie the disease process in glaucoma. This also strengthens the hope that glaucoma therapy beyond IOP lowering will become available. Implementing this concept with clinical trials remains, however, a challenge.

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“…The MS scan 1 automatic gain control target was set to 1e 6 , and the MS scan 2 target was set to 5e 5 with maximum ion injection times of 50 and 100 ms, respectively. Dynamic exclusion placed selected ions on an exclusion list for 30 s (50,51). Two different preparations of the complex were independently subjected to chemical cross-linking, limited proteolysis, and MS/MS as described above, the first in the absence and the second in the presence of the reducing agent, tris(2-carboxyethyl)phosphine (50 mM final concentration) (Hampton Research).…”

Section: Docking Of Yfh1mentioning

confidence: 99%

Architecture of the Yeast Mitochondrial Iron-Sulfur Cluster Assembly Machinery

Ranatunga

Gakh

Galeano

et al. 2016

Journal of Biological Chemistry

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The biosynthesis of Fe-S clusters is a vital process involving the delivery of elemental iron and sulfur to scaffold proteins via molecular interactions that are still poorly defined. Furthermore, molecular modeling suggests that two subunits of the cysteine desulfurase, Nfs1, may bind symmetrically on top of two adjacent Isu1 trimers in a manner that creates two putative [2Fe-2S] cluster assembly centers. In each center, conserved amino acids known to be involved in sulfur and iron donation by Nfs1 and Yfh1, respectively, are in close proximity to the Fe-S cluster-coordinating residues of Isu1. We suggest that this architecture is suitable to ensure concerted and protected transfer of potentially toxic iron and sulfur atoms to Isu1 during Fe-S cluster assembly.

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Proteome Analysis of Human Aqueous Humor

Cited by 156 publications

References 40 publications

Intraocular Biomarker Identification in Uveitis Associated With Juvenile Idiopathic Arthritis

Intraocular Biomarker Identification in Uveitis Associated With Juvenile Idiopathic Arthritis

Pharmacotherapy of Glaucoma

Architecture of the Yeast Mitochondrial Iron-Sulfur Cluster Assembly Machinery

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