Proteome analysis of human amniotic mesenchymal stem cells (hA-MSCs) reveals impaired antioxidant ability, cytoskeleton and metabolic functionality in maternal obesity

Abstract

Maternal obesity increases the risk of obesity and/or obesity-related diseases in the offspring of animal models. The aim of this study was to identify metabolic dysfunctions that could represent an enhanced risk for human obesity or obesity-related diseases in newborn or in adult life, similar to what occurs in animal models. To this aim, we studied the proteome of 12 obese (Ob-) and 6 non-obese (Co-) human amniotic mesenchymal stem cells (hA-MSCs) obtained from women at delivery by cesarean section (pre-pregnancy body mass index [mean ± SD]: 42.7 ± 7.7 and 21.3 ± 3.3 kg/m2, respectively). The proteome, investigated by two-dimensional fluorescence difference gel electrophoresis/mass spectrometry, revealed 62 differently expressed proteins in Ob- vs Co-hA-MSCs (P < 0.05), nine of which were confirmed by western blotting. Bioinformatics analysis showed that these 62 proteins are involved in several statistically significant pathways (P < 0.05), including the stress response, cytoskeleton and metabolic pathways. Oxidative stress was shown to be an early triggering factor of tissue fat accumulation and obesity-related disorders in the offspring of obese animal models. Our finding of a reduced stress response in Ob-hA-MSCs suggests that a similar mechanism could occur also in humans. Long-term follow-up studies of newborns of obese mothers are required to verify this hypothesis.

Label-Free Quantitative Proteomics in a Methylmalonyl-CoA Mutase-Silenced Neuroblastoma Cell Line

“…Cellular proteomes were resolved on a 10% Sodium Dodecyl Sulphate (SDS)-polyacrylamide gel ( Figure 3 ). Each gel lane was fractionated in order to obtain 40 fractions, which were cut and properly processed for protein identification by nanoLC-MS/MS [ 18 , 19 ]. The protein species identified by more than three peptides were taken into account and included in our proteomic dataset.…”

New insights on the functional role of URG7 in the cellular response to ER stress

“…The FLAG‐URG7 and mock lanes were cut in 35 slices per lane. Each slice was crushed and treated for in situ hydrolysis [Caterino et al ., ; Spaziani et al ., ; Capobianco et al ., ].…”

Proteomic Analysis of Mucopolysaccharidosis IIIB Mouse Brain

“…To investigate the molecular pathways influenced by NAGLU depletion in murine brain tissues, the identified proteomic dataset was analyzed by using the PANTHER (Protein ANalysis THrough Evolutionary Relationship) database available online at http://www.pantherdb.org [41][42][43] and the REACTOME database available online at https://www.reactome.org. Results of the PANTHER analyses for the biological process enrichment and pathway enrichment were expressed as percentage of protein listed in each category.…”