Proteome Analysis of Detached Fronds from a Resurrection Plant Selaginella Bryopteris - Response to Dehydration and Rehydration

Deeba, Farah; Pandey, Vivek; Pathre, Uday V.; Kanojiya, Sanjeev

doi:10.4172/jpb.1000067

Cited by 30 publications

(18 citation statements)

References 19 publications

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“…S. moellendorffii reached 25% RWC within 1 h, whereas S. lepidophylla required 4 h to reach this same point. This rate is similar to S. bryopteris , a related resurrection species that takes 6 h to lose 80–90% of its RWC (Deeba et al. , 2009; Pandey et al.…”

Section: Resultssupporting

confidence: 58%

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Comparative metabolic profiling between desiccation‐sensitive and desiccation‐tolerant species of Selaginella reveals insights into the resurrection trait

et al. 2012

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SUMMARYSpike mosses (Selaginellaceae) represent an ancient lineage of vascular plants in which some species have evolved desiccation tolerance (DT). A sister-group contrast to reveal the metabolic basis of DT was conducted between a desiccation-tolerant species, Selaginella lepidophylla, and a desiccation-sensitive species, Selaginella moellendorffii, at 100% relative water content (RWC) and 50% RWC using non-biased, global metabolomics profiling technology, based on GC/MS and UHLC/MS/MS 2 platforms. A total of 301 metabolites, including 170 named (56.5%) and 131 (43.5%) unnamed compounds, were characterized across both species. S. lepidophylla retained significantly higher abundances of sucrose, mono-and polysaccharides, and sugar alcohols than did S. moellendorffii. Aromatic amino acids, the well-known osmoprotectant betaine and flavonoids were also more abundant in S. lepidophylla. Notably, levels of c-glutamyl amino acid, linked with glutathione metabolism in the detoxification of reactive oxygen species, and with possible nitrogen remobilization following rehydration, were markedly higher in S. lepidophylla. Markers for lipoxygenase activity were also greater in S. lepidophylla, especially at 50% RWC. S. moellendorffii contained more than twice the number of unnamed compounds, with only a slightly greater abundance than in S. lepidophylla. In contrast, S. lepidophylla contained 14 unnamed compounds of fivefold or greater abundance than in S. moellendorffii, suggesting that these compounds might play critical roles in DT. Overall, S. lepidophylla appears poised to tolerate desiccation in a constitutive manner using a wide range of metabolites with some inducible components, whereas S. moellendorffii mounts only limited metabolic responses to dehydration stress.

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Section: Resultssupporting

confidence: 58%

“…Several members of the Selaginella genus, including S. lepidophylla (Iturriaga et al. , 2006), Selaginella bryopteris (Deeba et al. , 2009) and Selaginella tamariscina (Wang et al.…”

Section: Introductionmentioning

confidence: 99%

Comparative metabolic profiling between desiccation‐sensitive and desiccation‐tolerant species of Selaginella reveals insights into the resurrection trait

et al. 2012

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“…Uniquely, its detached fronds have the ability to survive in nearly complete desiccation similar to that of the whole plant. [ 17 18 ] Thus, in China some of these herbs are called longevity herbs and were thought to be beneficial for the human health. Studies have demonstrated that some Selaginella species have potent antitumor activities.…”

Section: Discussionmentioning

confidence: 99%

Comparison of cytotoxic activities of extracts from Selaginella species

Li¹,

Xiang²

2014

Phcog Mag

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Background:Selaginella species are resurrection plants, which are known, possess various molecular bioactivities depending on species, but only a few species have been detailed observe in the advanced research.Objective:The objective of the following study is to compare the chemical profiles of different species of Selaginella and to investigate cytotoxicity and induction of apoptosis activities of some species of Selaginella.Materials and Methods:The high-performance liquid chromatography (HPLC) method was developed for chemical analysis. Ethyl acetate, ethanol and water-soluble extracts from seven Selaginella species were submitted to 3-(4,5-dimenthyl thizol-2-yl)-2,5-diphenyl tetrazolium bromide assay, flow cytometry, deoxyribonucleic acid (DNA) laddering analysis and caspase-3 expression using Bel-7402, HT-29 and HeLa cells.Results:The HPLC analysis revealed two major common peaks, which were identified as amentoflavone and robustaflavone and another three main peaks in their chromatograms. The results showed that S. labordei, Selaginella tamariscina and Selaginella uncinata had relatively stronger activities on Bel-7402 and HeLa cells and Selaginella moellendorfii had moderate antiproliferation activities, but Selaginella remotifolia and Selaginella pulvinata had almost no inhibitory activities. The main active components were in the ethyl acetate extracts which had abundant biflavonoids. The effects of these extracts on cell proliferation and apoptosis in different cells were not the same, they were more apparent on HeLa cells than on HT-29 cells. The assay of DNA laddering analysis and caspase-3 expression further confirmed that inducing cell apoptosis was one of antitumor mechanisms and antitumor activities of Selaginella species were related to apoptosis induced by caspase family.Conclusion:S. labordei, S. tamariscina and S. uncinata would be potential antitumor agents.

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“…(10) In dehydration: Chemical analysis shows that the herb contains hexoses and proteins [17,18] suggest that S. bryopteris, has about 250 proteins that expressed in response to dehydration.…”

Section: (9) In Improvement Of Learning and Memorymentioning

confidence: 99%