Proteome analysis of chloroplast proteins in stage albinism line of winter wheat (triticum aestivum) FA85

Hou, Dan; Xu, Hong; Du, Guangyuan; Lin, Juntang; Duan, Min; Guo, Aizhen

doi:10.5483/bmbrep.2009.42.7.450

Cited by 32 publications

(31 citation statements)

References 30 publications

(20 reference statements)

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“…Heat-shock proteins/chaperones are responsible for protein folding, assembly, translocation and degradation in cellular processes and contribute to cellular homeostasis under stress conditions [32]. HSP 70 is essential for chloroplast development [33] and is down-regulated in a stage albinism line of winter wheat [20]. In our study, HSP 60 and HSP 70 were down-regulated, which implies that the protein processing system of White leaf No.…”

Section: Discussionmentioning

confidence: 60%

“…Transcriptomic analyses have identified many albinism-responsive genes and revealed a complex network involved in the albinism [16,18-20]. A transcriptomic analysis was also used for revealing the regulatory mechanism of changes in leaf color and amino acid levels during leaf development in White leaf No.…”

Section: Discussionmentioning

confidence: 99%

“…Glutamine synthetase (GS) catalyzes the formation of glutamine from glutamic acid, NH 3 and ATP. Expression of GS is suppressed by low-temperature stress in a stage albinism line of winter wheat [20] and is induced or reduced by salt stress in leaves of salt-tolerant and salt-sensitive rice, respectively [29]. Because White leaf No.…”

Section: Discussionmentioning

confidence: 99%

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Proteomic analysis of young leaves at three developmental stages in an albino tea cultivar

et al. 2011

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BackgroundWhite leaf No.1 is a typical albino tea cultivar grown in China and it has received increased attention in recent years due to the fact that white leaves containing a high level of amino acids, which are very important components affecting the quality of tea drink. According to the color of its leaves, the development of this tea cultivar is divided into three stages: the pre-albinistic stage, the albinistic stage and the regreening stage. To understand the intricate mechanism of periodic albinism, a comparative proteomic approach based on two-dimensional electrophoresis (2-DE) and mass spectrometry was adopted first time to identify proteins that changed in abundance during the three developmental periods.ResultsThe 2-DE results showed that the expression level of 61 protein spots varied markedly during the three developmental stages. To analyze the functions of the significantly differentially expressed protein spots, 30 spots were excised from gels and analyzed by matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry. Of these, 26 spots were successfully identified. All identified protein spots were involved in metabolism of carbon, nitrogen and sulfur, photosynthesis, protein processing, stress defense and RNA processing, indicating these physiological processes may play crucial roles in the periodic albinism. Quantitative real-time RT-PCR analysis was used to assess the transcriptional level of differentially expressed proteins. In addition, the ultrastructural studies revealed that the etioplast-chloroplast transition in the leaf cell of White leaf No. 1 was inhibited and the grana in the chloroplast was destroyed at the albinistic stage.ConclusionsIn this work, the proteomic analysis revealed that some proteins may have important roles in the molecular events involved in periodic albinism of White leaf No. 1 and identificated many attractive candidates for further investigation. In addition, the ultrastructural studies revealed that the change in leaf color of White leaf No. 1 might be a consequence of suppression of the etioplast-chloroplast transition and damage to grana in the chloroplast induced by temperature. These results provide much useful information to improve our understanding of the mechanism of albinism in the albino tea cultivar.

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

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Proteomic analysis of young leaves at three developmental stages in an albino tea cultivar

et al. 2011

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“…The wsl12 gene encodes a nucleoside diphosphate kinase 2 (OsNDPK2) that regulates chloroplast development and the abiotic stress response in rice (Ye et al, 2016). In wheat, the stage albinism line FA85 exhibits albinism in cold during early spring, but it reverts to green as the temperature increases gradually, where it is controlled by nuclear-cytoplasmic interaction (Hou et al, 2009). The WSL3 gene encodes an essential peripheral subunit of the plastid RNA polymerase complex (Wang et al, 2016a).…”

Section: Cytogenetic Analyses Of Psl1 Mutant a Novel Low-temperaturementioning

confidence: 99%

“…In Capsicum chinense Jacq., sy-2 exhibits chlorophyll deficiency at temperatures below 20°C, but normal development above 24°C . In wheat, FA85 exhibits albinism in all of the aerial parts in early spring, but it gradually becomes green as the temperature increases (Hou et al, 2009). The other low-temperature-sensitive mutant type is sensitive to cold temperatures, such as the conditions experienced by some overwintering cereals including wheat and barley.…”

Section: Diversity Of Low-temperature-sensitive Leaf Color Mutants Unmentioning

confidence: 99%

Cytogenetic Analyses of PSL1 Mutant, a Novel Low‐Temperature‐Sensitive Purple‐Striped Leaf Color Mutant in Wheat

Cong

Shi

et al. 2018

Crop Science

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Low‐temperature‐sensitive leaf color mutants are valuable materials for investigating the mechanisms related to chloroplast development and chlorophyll biosynthesis under low‐temperature conditions. PSL1 is a spontaneous low‐temperature‐sensitive purple‐striped leaf mutant of wheat (Triticum aestivum L.). The progeny of PSL1 exhibited three leaf color phenotypes under low‐temperature conditions (i.e., purple and albino leaf [pur], purple‐striped and albino‐striped leaf [pst], and normal green leaf [gre]). The leaf color of pur and pst displayed normal green under high temperature conditions. The leaf color of gre remained normal green under cold or high temperatures. The critical low temperature for leaf color variations in pur and pst was ∼5°C for >35 d. The shapes of chloroplasts in the purple and albino leaf sectors were irregular in pur and pst, where the thylakoid lamellar was undeveloped and no grana formed. The chlorophyll and carotenoid contents of pur and gre were remarkably reduced in the purple and albino periods, but the anthocyanin contents accumulated greatly in the same period. The photosynthetic capacities of the flag leaves in pur, pst, and gre were the same as that in the Xinong1125 during the heading stage. Genetic analysis showed that the leaf color mutations in PSL1 in response to temperature were controlled by cytoplasmic inheritance. Quantitative real‐time polymerase chain reaction showed that the psbA and psbC genes were downregulated most in the newly developed leaf tissues in pur and pst under low temperatures. This indicated that the psbA and psbC genes may be involved in chloroplast development under low‐temperature conditions in wheat.

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Comprehensive analysis of differently expressed genes and proteins in albino and green plantlets from a wheat anther culture

Zhao¹,

Wang²,

Zhang³

et al. 2017

Biologia plant.

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Proteome analysis of chloroplast proteins in stage albinism line of winter wheat (triticum aestivum) FA85

Cited by 32 publications

References 30 publications

Proteomic analysis of young leaves at three developmental stages in an albino tea cultivar

Proteomic analysis of young leaves at three developmental stages in an albino tea cultivar

Cytogenetic Analyses of PSL1 Mutant, a Novel Low‐Temperature‐Sensitive Purple‐Striped Leaf Color Mutant in Wheat

Comprehensive analysis of differently expressed genes and proteins in albino and green plantlets from a wheat anther culture

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