Proteome analysis of cerebrospinal fluid in Guillain–Barré syndrome (GBS)

Lehmensiek, Vera; Süssmuth, SD; Brettschneider, Johannes; Tauscher, G.; Felk, Sandra; Gillardon, Frank; Tumani, Hayrettin

doi:10.1016/j.jneuroim.2007.01.022

Cited by 40 publications

(34 citation statements)

References 20 publications

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“…Similar up-regulations of this multifunctional protein, which acts as a transporter of vitamin D sterols and fatty acids, by activating macrophage and chemotaxis, and as a scavenger [12], have been reported in many other neurological diseases [23,21,3,32]. Therefore, it is likely that vitamin D-binding protein up-regulation in CSF could represent a non-specific reaction to neurological inflammatory, or even non-inflammatory insults, with possible beneficial functions on post-inflammatory tissue repair.…”

supporting

confidence: 56%

“…Some proteins, such as transthyretin, apolipoprotein A-IV, vitamin D-binding protein, and haptoglobin, which were differentially regulated in the CSF of our GBS patients vs. controls, have been reported to be expressed in the CSF of patients with very different diseases, which range from neuromyelitis optica, a severe inflammatory CNS disease (transthyretin and vitamin D-binding protein [3]), and viral meningitis or multiple sclerosis (vitamin Dbinding protein [21]), to non-overtly-inflammatory CNS diseases, such as temporal lobe epilepsy (vitamin D-binding protein [32]), lumbar disk herniation (apolipoprotein A-IV and vitamin D-binding protein [23]), Alzheimer disease (transthyretin [26,11]), and amyotrophic lateral sclerosis (transthyretin [25]). As a result, it is likely that the relative-to-total-protein CSF concentration of these proteins is non-specifically influenced by very different neurological pathologies, and therefore it is very unlikely that they can be used as biomarkers in GBS.…”

mentioning

confidence: 88%

“…Recent proteomic studies showed that apolipoprotein A-IV and haptoglobin were up-regulated, whereas transthyretin and apolipoprotein E were down-regulated in the CSF of GBS patients [21,19,33,7].…”

mentioning

confidence: 99%

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Protein profiling of Guillain–Barrè syndrome cerebrospinal fluid by two-dimensional electrophoresis and mass spectrometry

D’Aguanno

Franciotta

Lupisella

et al. 2010

Neuroscience Letters

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a b s t r a c tProtein profiling of cerebrospinal fluid in Guillain-Barrè syndrome (GBS), an acute and immune-mediated disease affecting the peripheral nervous system, was performed by two-dimensional electrophoresis. Significant modulated spots in GBS patients vs. control groups (a group of multiple sclerosis patients and one of healthy donors) underwent MALDI-TOF/TOF investigation. Inflammation-related proteins, such as vitamin D-binding protein, beta-2 glycoprotein I (ApoH), and a complement component C3 isoform were up-regulated in GBS, whereas transthyretin (the monomer and the dimer forms), apolipoprotein E, albumin and five of its fragments were down-regulated. Then, we used an isoelectric-focusingdinitrophenylhydrazine-based technique to analyse the extent of carbonylation and, as a result, of oxidative damage of GBS CSF proteome. We observed a major sensitivity to carbonylation for albumin and alpha-glycoprotein in inflammation and a selective increase of reactivity for a glycosylated Fab from an IgM globulin in GBS CSF. Our results add new proteins to candidate CSF features of GBS, and suggest that oxidative stress could contribute to the immunopathological mechanisms in this syndrome.

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supporting

confidence: 56%

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confidence: 88%

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Protein profiling of Guillain–Barrè syndrome cerebrospinal fluid by two-dimensional electrophoresis and mass spectrometry

D’Aguanno

Franciotta

Lupisella

et al. 2010

Neuroscience Letters

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“…However, there have been no comprehensive analyses of the canine CSF proteome. In humans, analyses of the CSF proteome have been used to search for diagnostic markers in patients with multiple sclerosis, Guillain-Barré syndrome and Alzheimer's disease [1,4,5,11,16,17]. The search for biomarkers of neurological diseases in dogs will require proteomic analyses of CSF in normal dogs.…”

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confidence: 99%

Proteome Analysis of Cerebrospinal Fluid in Healthy Beagles and Canine Encephalitis

Nakamura

Miyasho

Nomura

et al. 2012

The Journal of Veterinary Medical Science

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AbSTRAcT. We performed proteomics analysis of the cerebrospinal fluid (CSF) of healthy dogs and dogs with meningoencephalitis of unknown etiology (MUE). By comparing two-dimensional electrophoreses (2DE), an upregulated spot was found in MUE dogs. This protein was identified as a neuron-specific enolase (NSE) by analysis with MALDI-TOF mass spectrometry. In comparing dot blots using an antibody against NSE, the NSE levels in the CSF of MUE dogs was significantly higher than that of the controls. NSE is a diagnostic marker of neuroendocrine tumors, brain injury and spinal cord trauma in humans. It seems that the NSE concentration in the CSF is increased by cellular destruction in canine encephalitis. Though elevation of NSE may not be specific in canine encephalitis because the NSE level was increased in other CNS diseases, further study including measurement with serum is necessary.

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“…In Guillain-Barré syndrome (GBS) high levels of serum Hp were identified and attributed to the concomitant raise in Interleukin 6 (IL 6) 8 . More recently, elevation of Hp levels in the cerebrospinal fluid (CSF) of GBS patients was reported 9 .…”

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confidence: 99%

Haptoglobin study in myasthenia gravis

Oliveira

França

Nucci

et al. 2008

Arq. Neuro-Psiquiatr.

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-Objective: A cross-sectional study of haptoglobin (Hp) in myasthenia gravis (MG) was designed, with the objective to identify its values and correlate them with different disease status. Method: 46 patients were enrolled in the study, all having disease severity established according to the quantitative myasthenia gravis strength scores (QMGSS). Based on the functional scale determined by Myasthenia Gravis Foundation of America (MGFA) recommendations, patients were classified as having: complete stable remission (CSR; n=10); minimal manifestations-0 (MM0; n=6), minimal manifestations-1 (MM1; n=4); pharmacological remission (PR; n=6). Two other groups participated: thymomatous patients (T; n=10) and patients without imunosuppression or thymectomy, until the assessment for Hp (WIT; n=10). Hp dosage was done by immunonephelometry, blindly to clinical data. Student´s t-test, Anova test and linear regression were employed for statistical analyses. Results: Statistically significant differences occurred between CSR+MM0xWIT groups (86.62x157.57, p<0.001) and PR+MM1xWIT groups (73.93x157.57, p<0.001). Linear regression showed correlation between Hp levels and QMGSS (r=0.759, p<0.001). Conclusion: Our results suggest that Hp may be useful in clinical practice as a disease severity marker in MG.KEY WORDS: haptoglobin, acute phase response, myasthenia gravis, immune disorders. estudo sobre a haptoglobina na miastenia graveResumo -Objectivo: Desenhou-se estudo transversal sobre a haptoglobina (Hp) na miastenia grave (MG) com o objetivo de identificar seus valores e correlacioná-los a diferentes condições na doença. Método: 46 pacientes foram incluídos, todos tendo a gravidade da doença estabelecida segundo escores internacionais (QMGSS). Os pacientes tiveram seu estado funcional determinado de acordo com a Myasthenia Gravis Foundation of América (MGFA) e classificados em: remissão completa estável (CSR; n=10); mínima manifestação-0 (MM0; n=6), mínima manifestação-1 (MM1; n=4); remissão farmacológica (PR; n=6). Dois outros grupos participaram: pacientes timomatosos (T; n=10) e pacientes sem imunossupressão ou timectomia, até o momento da inclusão no estudo (WIT; n=10). A dosagem de Hp foi realizada por imunonefelometria, de modo cego quanto à clínica. As análises estatísticas incluíram o teste de Student, Anova e regressão linear. Resultados: Observou-se diferença significativa entre os grupos CSR+MM0xWIT (86,62x157,57, p<0,001) e entre PR+MM1xWIT (73,93x157,57, p<0,001). A regressão linear mostrou correlação positiva entre os valores de Hp e os escores QMGSS (r=0,759, p<0,001). Conclusão: O estudo sugere que valores altos de Hp se correlacionaram a maior gravidade da MG. PALAVRAS-CHAVE: haptoglobina, resposta de fase aguda, miastenia grave, doença autoimune.

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Proteome analysis of cerebrospinal fluid in Guillain–Barré syndrome (GBS)

Cited by 40 publications

References 20 publications

Protein profiling of Guillain–Barrè syndrome cerebrospinal fluid by two-dimensional electrophoresis and mass spectrometry

Protein profiling of Guillain–Barrè syndrome cerebrospinal fluid by two-dimensional electrophoresis and mass spectrometry

Proteome Analysis of Cerebrospinal Fluid in Healthy Beagles and Canine Encephalitis

Haptoglobin study in myasthenia gravis

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