Proteome analysis of Bacillus subtilis extracellular proteins: a two-dimensional protein electrophoretic study The SWISS-PROT accession numbers for the N-terminal amino acid sequences reported in this paper are: P00691 for AmyE; P54507 for CotN; O07921 for Csn; P09124 for Gap; P26901 for KatA; P39116 for Pel; P39824 for PenP; P54375 for SodA; P29141 for Vpr; Q07833 for WapA; P54423 for WprA; P54327 for XkdG; Q45071 for XynD; P94421 for YclQ; O31803 for YcnM; O05512 for YdhT; O34952 for YflE; O06487 for Yfn

Abstract

To analyse the proteome of Bacillus subtilis extracellular proteins, extracellular protein samples were prepared from culture media (minimal medium containing 04 % glucose) of parental B. subtilis 168, a secA-temperature sensitive mutant and an ffh conditional mutant, and examined by twodimensional gel electrophoresis. Approximately 100 to 110 spots were visualized in a gel of B. subtilis 168 extracellular proteins. Over 90 % and 80 % of these disappeared in the absence of SecA and Ffh, respectively. Thirty-eight obvious spots on the gel of the B. subtilis 168 preparation were selected and compared with spots obtained under SecA-or Ffh-deficient conditions. The appearance of 36 of these 38 spots depended on SecA and Ffh. Nineteen additional extracellular proteins were detected in cultures maintained in cellobiose, maltose and soluble starch. Among 23 proteins of which the Nterminal amino acid sequences were determined, 17 were extracellular proteins having signal peptides in their precursor form. Two membrane proteins, YfnI and YflE, were cleaved behind 226 Ala-Tyr-Ala 228 and 213 Ala-LeuAla 215 , respectively, and of which products seemed to be liberated into the culture medium. The production of YfnI and YflE were also dependent on SecA and Ffh. These results indicate that most extracellular proteins target to and translocate across the cytoplasmic membrane by co-operation between the signal-recognition particle and Sec protein-secretion pathways. In contrast, a spot for Hag appeared independent from SecA and Ffh. Intracellular proteins Gap, SodA and KatA were identified in the extracellular protein samples. On the basis of these results and computer searches, it was predicted that B. subtilis produces 150 to 180 proteins extracellularly.

Selective Contribution of the Twin-Arginine Translocation Pathway to Protein Secretion in Bacillus subtilis

“…SecA-dependent Secretion of Overproduced LipA-The Secdependent pathway of B. subtilis can be regarded as the major route for protein secretion into the growth medium (2,39). Therefore, we investigated whether the Tat-independently secreted LipA was transported in a Sec-dependent manner.…”

“…Proteomic Evaluation of Sec-dependent Protein SecretionPrevious proteomic studies by Hirose et al (39) suggested that the secretion of the majority of extracellular proteins by B. subtilis is strongly SecA-dependent. However, the interpretation of these results was complicated by the fact that a temperature-sensitive secA mutant strain was used, which stops growing and dies upon temperature upshift.…”

Biochemical properties and three-dimensional structures of two extracellular lipolytic enzymes from Bacillus subtilis

“…Activities were determined using (1) , spectrophotometric; (2) , pH-stat; (3) , monolayer assays. the triacylglyceride tricaprylin (C8:0).…”

“…was about 10% for each given value. Activities were determined using the following assays, (1) , pH-stat; (2) , UVspectrophotometric; (3) , fluorescent; (4) , monolayer; (5) , spectrophotometric.…”

“…Bacillus subtilis is one of the best studied microorganisms with respect to its genome, proteome and secretome [1][2][3]. Its genome sequence published in 1997 by Kunst et al [1] represented the first complete genome sequenced from a Grampositive bacterium.…”