Proteome Analysis of B. subtilis in Response to Calcium

Domniguez, Delfina C

doi:10.4172/2155-9872.s6-001

Cited by 11 publications

(17 citation statements)

References 55 publications

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“…These data were in accordance with those reported in a previous study demonstrating that EF-Tu of B. subtilis can bind to calcium ions 22 . In addition, Day and coworker 23 have shown that EF-Tu also has EF-hand domains via the genomic analysis of the EF-hand related sequences in Arabidopsis .…”

Section: Resultssupporting

confidence: 93%

Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation

Amimanan

Tavichakorntrakool

Fong-ngern

et al. 2017

Sci Rep

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Escherichia coli is the most common bacterium isolated from urine and stone matrix of calcium oxalate (CaOx) stone formers. Whether it has pathogenic role(s) in kidney stone formation or is only entrapped inside the stone remains unclear. We thus evaluated differences between E. coli isolated from urine of patients with kidney stone (EUK) and that from patients with urinary tract infection (UTI) without stone (EUU). From 100 stone formers and 200 UTI patients, only four pairs of EUK/EUU isolates had identical antimicrobial susceptibility patterns. Proteomic analysis revealed nine common differentially expressed proteins. Among these, the greater level of elongation factor Tu (EF-Tu) in EUK was validated by Western blotting. Outer membrane vesicles (OMVs) derived from EUK had greater promoting activities on CaOx crystallization, crystal growth and aggregation as compared to those derived from EUU. Neutralizing the OMVs of EUK with monoclonal anti-EF-Tu antibody, not with an isotype antibody, significantly reduced all these OMVs-induced promoting effects. Moreover, immunofluorescence staining of EF-Tu on bacterial cell surface confirmed the greater expression of surface EF-Tu on EUK (vs. EUU). Our data indicate that surface EF-Tu and OMVs play significant roles in promoting activities of E. coli on CaOx crystallization, crystal growth and aggregation.

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Section: Resultssupporting

confidence: 93%

Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation

Amimanan

Tavichakorntrakool

Fong-ngern

et al. 2017

Sci Rep

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“…In support, in prokaryotes, Ca 2+ has been implicated in various physiological processes such as spore formation, motility, cell differentiation, transport, and virulence (reviewed in [5]). It has also been shown that Ca 2+ modulates bacterial gene expression [6-8], suggesting its regulatory role in prokaryotes. Furthermore, there is growing evidence that Ca 2+ plays a signaling role in prokaryotes, which requires a tight control of cellular Ca 2+ homeostasis.…”

Section: Introductionmentioning

confidence: 99%

Calcium homeostasis in Pseudomonas aeruginosa requires multiple transporters and modulates swarming motility

et al. 2013

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Pseudomonas aeruginosa is an opportunistic human pathogen causing severe acute and chronic infections. Earlier we have shown that calcium (Ca2+) induces P. aeruginosa biofilm formation and production of virulence factors. To enable further studies of the regulatory role of Ca2+, we characterized Ca2+ homeostasis in P. aeruginosa PAO1 cells. By using Ca2+-binding photoprotein aequorin, we determined that the concentration of free intracellular Ca2+ ([Ca2+]in) is 0.14±0.05 μM. In response to external Ca2+, the [Ca2+]in quickly increased at least 13 fold followed by a multi-phase decline by up to 73%. Growth at elevated Ca2+ modulated this response. Treatment with inhibitors known to affect Ca2+ channels, monovalent cations gradient, or P-type and F-type ATPases impaired [Ca2+]in response, suggesting the importance of the corresponding mechanisms in Ca2+ homeostasis. To identify Ca2+ transporters maintaining this homeostasis, bioinformatic and LC-MS/MS-based membrane proteomic analyses were used. [Ca2+]in homeostasis was monitored for seven Ca2+-affected and eleven bioinformatically predicted transporters by using transposon insertion mutants. Disruption of P-type ATPases PA2435, PA3920, and ion exchanger PA2092 significantly impaired Ca2+ homeostasis. The lack of PA3920 and vanadate treatment abolished Ca2+- induced swarming, suggesting the role of the P-type ATPase in regulating P. aeruginosa response to Ca2+.

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“…These proteins were induced when cells were treated with extracellular divalent cation chelator ethylene glycol tetraacetic acid (EGTA) and reduced when treated with high extracellular Ca 2+ . None of these proteins however had Ca 2+ binding domains [32]. Notably genes encoding fructose biposhate aldolase, DnaK 70 and adenylate kinase were found to be modulated by Ca 2+ in E. coli [20].…”

Section: Ca 2+ Homeostasis In Bacteriamentioning

confidence: 97%

“…However, recent work shows that the disruption of particular ATPases (PA2435, PA3920), the exchanger (PA2092) and a putative EF-hand protein, is evidence that these transporters are necessary to maintain low intracellular Ca 2+ levels in P. aeruginosa [15,38]. A proteomic analysis in B. subtilis showed that several cytosolic proteins appear to bind Ca 2+ , as determined by Ca 2+ autoradiography [32]. Some of these proteins, identified by liquid chromatography/ mass spectrometry include: a potential cation transport ATPase, fructose biposhate aldolase, DnaK 70 and adenylate kinase.…”

Section: Ca 2+ Homeostasis In Bacteriamentioning

confidence: 99%

“…There is evidence of calcium binding proteins (CaBP) in several genera of bacteria, including proteins with EF-hand domains [25,26], and other calcium motifs such as β-rolls motif, Greek key motif, repeats in toxin and Big Ca 2+ domain [27][28][29][30] but their functional role needs to be investigated. Proteomic and transcriptomic studies in E. coli, Calcium and Signal Transduction P. aeruginosa and B. subtilis showed that hundreds of genes and proteins are up/downregulated by changes in ([Ca 2+ ] i ) but the physiological role needs to be elucidated [20,21,23,31,32]. Figure 1 illustrates the potential roles of Ca 2+ in prokaryotes.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Calcium Signaling in Prokaryotes

Domı́nguez¹

2018

Calcium and Signal Transduction

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Calcium (Ca 2+) functions as a universal messenger in eukaryotes and regulates many intracellular processes such as cell division and gene expression. However, the physiological role of Ca 2+ in prokaryotic cells remains unclear. Indirect evidence suggests that Ca 2+ is involved in a wide variety of bacterial cellular processes including membrane transport mechanisms (channels, primary and secondary transporters), chemotaxis, cell division and cell differentiation processes such as sporulation and heterocyst formation. In addition, Ca 2+ signaling has been implicated in various stages of bacterial infections and host-pathogen interactions. The most significant discovery is that similar to eukaryotic cells, bacteria always maintain very low cytosolic free Ca 2+ , even in the presence of millimolar extracellular Ca 2+. Furthermore, Ca 2+ transients are produced in response to stimuli by several agents. Transport systems, which may be involved in Ca 2+ homeostasis are present in bacteria but none of these have been examined critically. Ca 2+-binding proteins have also been identified, including proteins with EF motifs but their role as intracellular Ca 2+ targets is elusive. Genomic studies indicate that changes in intracellular Ca 2+ up and downregulate hundreds of genes and proteins suggesting a physiological role. This chapter presents an overview of the role of Ca 2+ in prokaryotes summarizing recent developments.

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Proteome Analysis of B. subtilis in Response to Calcium

Cited by 11 publications

References 55 publications

Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation

Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation

Calcium homeostasis in Pseudomonas aeruginosa requires multiple transporters and modulates swarming motility

Calcium Signaling in Prokaryotes

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