Proteome analysis of Arabidopsis seedlings exposed to bacterial volatiles

Kwon, Young Chul; Ryu, Choong‐Min; Lee, Mi Kyung; Park, Hyo Bee; Han, Ki Soo; Lee, Jung Han; Lee, Kyung‐Hee; Chung, Woo Sik; Jeong, Mi‐Jeong; Kim, Hee Kyu; Bae, Dong-Won

doi:10.1007/s00425-010-1259-x

Cited by 135 publications

(91 citation statements)

References 49 publications

(77 reference statements)

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“…The importance of the ethylenedependent signaling pathway for elicitation of ISR by VOCs from strain GB03 was confirmed in tests using GUS fusions to the PDF1.2 gene, which is an indicator for the ethylene response. Recently, the relationship between ethylene signaling and bacterial VOC-elicited ISR was further substantiated by proteomic analysis that demonstrated significant upregulation of three major ethylene biosynthesis proteins (i.e., aspartate aminotransferase, S-adenosylmethionine synthetase 2, and methionine adenosyltransferase 3) following bacterial VOC treatment (31).…”

Section: Production and Effects Of Bacterial Volatilesmentioning

confidence: 99%

The Multifactorial Basis for Plant Health Promotion by Plant-Associated Bacteria

Kim

Leveau

Gardener

et al. 2011

Appl Environ Microbiol

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225

138

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7On plants, microbial populations interact with each other and their host through the actions of secreted metabolites. However, the combined action of diverse organisms and their different metabolites on plant health has yet to be fully appreciated. Here, the multifactorial nature of these interactions, at the organismal and molecular level, leading to the biological control of plant diseases is reviewed. To do so, we describe in detail the ecological significance of three different classes of secondary metabolites and discuss how they might contribute to biological control. Specifically, the roles of auxin, acetoin, and phenazines are considered, because they represent very different but important types of secondary metabolites. We also describe how studies of the global regulation of bacterial secondary metabolism have led to the discovery of new genes and phenotypes related to plant health promotion. In conclusion, we describe three avenues for future research that will help to integrate these complex and diverse observations into a more coherent synthesis of bacterially mediated biocontrol of plant diseases.Diverse plant-associated bacteria can positively impact plant health and physiology in a variety of ways (19). Three wellstudied mechanisms of biological disease control and plant health promotion conferred by plant-associated bacteria have recently been reviewed (38). However, the focus on identifying and characterizing individual mechanisms has obscured the complex, multifactorial nature of biological control. So, while many different biocontrol bacteria have been identified and much has been learned about different types of plant-bacterium interactions for some of these populations, we are just beginning to appreciate the number and complexity of interactions actually taking place in situ. Indeed, many different bacteria and many different bacterial metabolites have been identified as important contributors to the biological control of plant diseases. However, we still lack a clear understanding of how the populations and activities of the diverse populations of microorganisms that colonize every plant are connected and integrated in natural and agricultural environments.Here, we review recent work that indicates the multifactorial nature of biocontrol (Fig. 1). Specifically, we review a number of the studies indicating that biocontrol arises from the combined actions of multiple bacterial populations, each expressing several different classes of bioactive metabolites under the control of multiple genes and regulons. And we highlight recent work in the authors' laboratories that begins to identify the diverse factors contributing to changes in plant growth and health status. We conclude by providing a road map for future research that will lead to a greater understanding of the complex, dynamic, and multifactorial nature of biological control phenotypes expressed by plant-associated bacteria.

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Section: Production and Effects Of Bacterial Volatilesmentioning

confidence: 99%

The Multifactorial Basis for Plant Health Promotion by Plant-Associated Bacteria

Kim

Leveau

Gardener

et al. 2011

Appl Environ Microbiol

Self Cite

225

138

View full text Add to dashboard Cite

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“…The analysis of Arabidopsis proteome when exposed to VOCs from B . subtilis GB03 showed upregulation of ethylene biosynthesis, antioxidant proteins, and proteins in jasmonic acid (JA) and SA signaling (Kwon et al 2010 ). Rhizobacterial VOCs not only promote plant growth but also play a role in ISR: 2,3-butanediol from B .…”

Section: Arabidopsis Model To Study Host-endophyte Symbiosismentioning

confidence: 99%

Unraveling the Dark Septate Endophyte Functions: Insights from the Arabidopsis Model

Mandyam

Jumpponen

2013

Advances in Endophytic Research

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The global occurrence of plant root-associated fungal endophytes and their great abundance in many habitats necessitate studies to decipher their potential functions. Improved understanding of the basic endophyte ecology including host range, host preference, and host responses to endophyte colonization has been made possible through populations of endophytes (e.g., Periconia macrospinosa and Microdochium sp.) isolated from North American native tallgrass prairie. The recent demonstration of the endophyte symbiosis of the model plant Arabidopsis thaliana has provided additional tools to further elucidate the ecology of these endophytes. The availability of a large number of Arabidopsis ecotypes and mutants, microarrays, and databases allows the molecular dissection of endophyte symbiosis to better understand the importance of fungal endophytes in host nutrient uptake, defenses, and/or responses to pathogens and stress. In this chapter, we discuss the ecology and functions of endophytic fungi through experiments utilizing the Arabidopsis model system. We draw parallels with another deeply dissected Piriformospora indica root endophyte symbiosis, which has been demonstrated to promote growth of model and non-model plants.

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“…2-D gel electrophoresis and image analysis 2-D gel electrophoresis was performed as previously described (Kwon et al 2010). Briefly, proteins (100 µg) were separated using a 17-cm pH 5-8 IPG strip in a Protean IEF cell (Bio-Rad, Hercules, CA, USA) and in-gel rehydrated for 12 h. Isoelectric focusing (IEF) was performed at 20℃ for 15 min at 250 V, 3 h at 10,000 V, and 9 h at 90,000 V. The strips were equilibrated using equilibrating solution [50 mMTris-HCL (pH 8.8), 6 M urea, 30% glycerol, 2% SDS] containing 1% DTT and then incubated with the same buffer containing 2.5% iodoacetamide.…”

Section: Protein Extraction and Quantificationmentioning

confidence: 99%

Comparative proteomic analysis of plant responses to sound waves in Arabidopsis

Kwon¹,

Jeong²,

Cha³

et al. 2012

Journal of Plant Biotechnology

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Environmental factors greatly influence the growth, development, and even genetic characteristics of plants. The mechanisms by which sound influences plant growth, however, remain obscure. Previously, our group reported that several genes were differentially regulated by specific frequenciesof sound treatmentusing a sound-treated subtractive library. In this study, we used a proteomic approach to investigate plant responses to sound waves in Arabidopsis. The plants were exposed to 250-Hz or 500-Hz sound waves, and total proteins were extracted from leaves 8 h and 24 h after treatment. Proteins extracted from leaves were subjected to 2-DE analysis. Thirty-eight spots were found to be differentially regulated in response to sound waves and were identified using MALDI-TOF MS and MALDI-TOF/TOF MS. The functions of the identified proteins were classified into photosynthesis, stress and defense, nitrogen metabolism, and carbohydrate metabolism. To the best of our knowledge, this is the first report on the analysis of protein changes in response to sound waves in Arabidopsis leaves. These findings provide a better understanding of the molecular basis of responses to sound waves in Arabidopsis.

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Proteome analysis of Arabidopsis seedlings exposed to bacterial volatiles

Cited by 135 publications

References 49 publications

The Multifactorial Basis for Plant Health Promotion by Plant-Associated Bacteria

The Multifactorial Basis for Plant Health Promotion by Plant-Associated Bacteria

Unraveling the Dark Septate Endophyte Functions: Insights from the Arabidopsis Model

Comparative proteomic analysis of plant responses to sound waves in Arabidopsis

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