Proteome analysis for rat saliva

Abstract

Proteome analysis is a popular method to discover biomarkers for the prevention and diagnosis of diseases. Since saliva is a non-invasively available body fluid, gathering of saliva causes minimal harm to patients. Therefore, detection of proteins for the prevention and diagnosis from the saliva sample may be the preferred method, especially for children and elderly people. However, the abundance of salivary proteins and contaminant proteins from food and mouth bacteria obscure identification of proteins present in the saliva at low concentrations. To address this problem, we developed a shotgun proteomic method using two-dimensional nano-flow LC tandem mass spectrometry. We report here that our method is able to detect proteins quantitatively even in small sample volumes of saliva.

Effects of chronic dietary exposure to monosodium glutamate on feeding behavior, adiposity, gastrointestinal motility, and cardiovascular function in healthy adult rats

“…However, serous and mucous acini in the submaxilar salivary gland were not altered. Further research is needed to determine whether saliva composition is affected by daily MSG intake.…”

Positive Selection Shaped the Convergent Evolution of Independently Expanded Kallikrein Subfamilies Expressed in Mouse and Rat Saliva Proteomes

“…A human salivary proteome has been produced by a similar method to that we report here [21] and, in addition, the UCLA Human Saliva Proteome Project (HSPP) is available on-line at the Salivaomics Knowledge Database (SKB) (http://www.skb.ucla.edu/). No similar resource is available for rodent saliva proteomes although a brief description of a shotgun proteomic method using two-dimensional nano-flow LC tandem mass spectrometry to study rat salivas appeared recently [22].…”