“…223 and 224 were mixed and used for immunoelectrophoresis. 4 Fibrin-agar-electrophoresis: The leukocyte extract was subjected to electrophoresis in fibrin-agar-plates (7.6 cm X 2.5 cm) according to the method originally described (16,17). The optimal pH value for fibrin digestion was determined by incubating the plates, after electrophoresis, for 30 to 60 min at 22°C in buffer solutions of different pH values.…”
“…223 and 224 were mixed and used for immunoelectrophoresis. 4 Fibrin-agar-electrophoresis: The leukocyte extract was subjected to electrophoresis in fibrin-agar-plates (7.6 cm X 2.5 cm) according to the method originally described (16,17). The optimal pH value for fibrin digestion was determined by incubating the plates, after electrophoresis, for 30 to 60 min at 22°C in buffer solutions of different pH values.…”
“…serum mono-n-butyrin and liver esterase (5) Proteinases inhibited by reducing agents (a) Trypsin (kinase said to be inhibited) (34) (b) Proteinases of aerobic bacteria in absence of Fe ++ (85) Peptidascs inhibRed by reducing agents (a) Some yeast and mold dipeptidases (also inhibited by Mn ++ and polypeptidases (33, 10) (b) Intestinal leucine aminopeptidase (33), effect counteracted by Mn ++ and Mg ++ (9) (c) Papain peptidase (12) Other enzyme systems inhibited by reducing agents (a) Protein synthesis by papain (80, not confirmed in 77) at pH 8. Human polymorphs (obtained from the blood of myeloid leukemia patients) have been found to contain proteinase most active at pH 7 to 8, other proteinase most active at pH 4, and peptidase most active at pH 7 to 8 (Husfeldt, 1931).…”
Section: S --Me-~-h + Active Inactivementioning
confidence: 99%
“…Peptidase activity has been found in extracts of human polymorphonuclear leucocytes (Husfeldt, 1931). Human serum has also been found to contain a small amount of active peptidase (Jobling and Strouse, 1912).…”
Section: The Control Of Tiie Activity Of Leucopeptidase (Experim'ementioning
Because enzymes are among the most important regulators of animal and plant life processes, the results of the ability to control and utilize them may well prove to be far reaching. Such control and utilization can only follow an understanding of their mode of action, and of the conditions affecting their activity.One of the most important and best studied groups of organic catalysts is the proteolytic enzymes.
“…In guter tTbereinstimmung mit den Angaben , dass in Kaninchen Leukocyten nur die starke Kathepsinwirkung auftrat, die Trypsin wirkung aber vermisst wurde (Dernby (1918)2), Husfeldt (1931)3), Kleinmann und Scharr (1932)4)), hat Verf. daraus angenommen, dass bei der akut verlaufenden allergischen Pneumonie die merkwar dige Steigerung der Leukocyteninfiltration und die autolytische sowie heterolytische Katheptasewirkung in naher Beziehung stehen musse u nd Bass die Anhaufung der Leukocyten im Lungengewebe an der Steigerung der Katheptasewirkung teilnehmen musse .…”
Section: (Aus Dem Medizinisch-chemischen Institut Der Medizinischenunclassified
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