Proteolytic Processing of SERK3/BAK1 Regulates Plant Immunity, Development, and Cell Death

Zhou, Jianguang; Wang, Ping; Claus, Lucas Alves Neubus; Savatin, Daniel V.; Xu, Guangyuan; Wu, Shu-Jing; Meng, Xiangzong; Russinova, Eugenia; He, Ping; Shan, Libo

doi:10.1104/pp.18.01503

Cited by 50 publications

(33 citation statements)

References 86 publications

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“…Association with different RLKs leads to different BAK1 phosphorylation code, explaining specificity of different RLKs in plant immunity and growth/development. Besides, the Ca 2+ ‐dependent proteolytic cleavage process of BAK1 is conserved in multiple plants, and the homeostasis of BAK1 mediated by a proteolytic process is also important for plant immunity, growth, and development (Zhou et al 2019). Elucidation of these codes of post‐translational modification for signaling specificity will improve our understanding of PRRs.…”

Section: Recent Discoveries Of the Activation Of Immunity Mediated Bymentioning

confidence: 99%

Plant immune signaling: Advancing on two frontiers

Wang

Feng

Zhou

et al. 2020

JIPB

176

144

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Plants have evolved multiple defense strategies to cope with pathogens, among which plant immune signaling that relies on cell-surface localized and intracellular receptors takes fundamental roles. Exciting breakthroughs were made recently on the signaling mechanisms of pattern recognition receptors (PRRs) and intracellular nucleotide-binding site (NBS) and leucine-rich repeat (LRR) domain receptors (NLRs). This review summarizes the current view of PRRs activation, emphasizing the most recent discoveries about PRRs' dynamic regulation and signaling mechanisms directly leading to downstream molecular events including mitogen-activated protein kinase (MAPK) activation and calcium (Ca 2+ ) burst. Plants also have evolved intracellular NLRs to perceive the presence of specific pathogen effectors and trigger more robust immune responses. We also discuss the current understanding of the mechanisms of NLR activation, which has been greatly advanced by recent breakthroughs including structures of the first full-length plant NLR complex, findings of NLR sensor-helper pairs and novel biochemical activity of Toll/interleukin-1 receptor (TIR) domain.

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Section: Recent Discoveries Of the Activation Of Immunity Mediated Bymentioning

confidence: 99%

Plant immune signaling: Advancing on two frontiers

Wang

Feng

Zhou

et al. 2020

JIPB

176

144

View full text Add to dashboard Cite

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“…Notably, tyrosine phosphorylation is also essential for BAK1 function in immune signaling (Perraki et al , ). BAK1 is a co‐receptor for many LRR‐RKs and functions in plant immunity, growth, and development (de Oliveira et al , ; Yamada et al , ; Peng et al , ; Zhou et al , ). A recent study showed that Y403 phosphorylation is required for BAK1 function in immune signaling, highlighting the importance of tyrosine phosphorylation for this immune protein (Perraki et al , ).…”

Section: Discussionmentioning

confidence: 99%

Tyrosine phosphorylation of the lectin receptor‐like kinase LORE regulates plant immunity

Luo

Liang

et al. 2020

The EMBO Journal

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Plant pattern recognition receptors (PRRs) perceive pathogen‐associated molecular patterns (PAMPs) to activate immune responses. Medium‐chain 3‐hydroxy fatty acids (mc‐3‐OH‐FAs), which are widely present in Gram‐negative bacteria, were recently shown to be novel PAMPs in Arabidopsis thaliana. The Arabidopsis PRR LIPOOLIGOSACCHARIDE‐SPECIFIC REDUCED ELICITATION (LORE) is a G‐type lectin receptor‐like kinase that recognizes mc‐3‐OH‐FAs and subsequently mounts an immune response; however, the mechanisms underlying LORE activation and downstream signaling are unexplored. Here, we report that one of the mc‐3‐OH‐FAs, 3‐OH‐C10:0, induces phosphorylation of LORE at tyrosine residue 600 (Y600). Phosphorylated LORE subsequently trans‐phosphorylates the receptor‐like cytoplasmic kinase PBL34 and its close paralogs, PBL35 and PBL36, and therefore activates plant immunity. Phosphorylation of LORE Y600 is required for downstream phosphorylation of PBL34, PBL35, and PBL36. However, the Pseudomonas syringae effector HopAO1 targets LORE, dephosphorylating the tyrosine‐phosphorylated Y600 and therefore suppressing the immune response. These observations uncover the mechanism by which LORE mediates signaling in response to 3‐OH‐C10:0 in Arabidopsis.

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“…The in vitro kinase activity assay was performed as previous described (Zhou et al, 2019). In brief, reactions were performed in 30 µl of kinase buffer [20 mM Tris-HCl at pH 7.5, 10 mM MgCl 2 , 5mM EGTA, 100 mM NaCl, and 1 mM dithiothreitol containing 10 µg fusion proteins with 0.1 mM cold ATP and 5 µCi ( 32 P) γ-ATP] at room temperature for 3 h with gentle shaking.…”

Section: Methodsmentioning

confidence: 99%

BIK1 and ERECTA Play Opposing Roles in Both Leaf and Inflorescence Development in Arabidopsis

Chen

Liu

et al. 2019

Front. Plant Sci.

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Plants employ cell-surface receptor-like kinases to detect extrinsic and intrinsic signals, thus make a trade-off between growth and immunity. The receptor-like cytoplasmic kinases on the cytoplasmic side act as downstream components involved in the activation, transmission, and integration of intracellular signals. In Arabidopsis thaliana, the RLCK BOTRYTIS-INDUCED KINASE1 (BIK1) associates with multiple RLKs to regulate pathogen defense responses and brassinosteroid (BR) signaling. However, little is known about the biological functions of BIK1 in developmental processes in Arabidopsis. In this study, we established that mutation of ERECTA (ER), an important RLK, counteracts the developmental effects of loss of BIK1 function. BIK1 and ER play opposing roles in leaf morphogenesis and inflorescence architecture. Moreover, we confirmed that BIK1 is required to maintain appropriate auxin response during leaf margin morphogenesis. Finally, we found that BIK1 interacts with ER-family proteins and directly phosphorylates ER. Our findings might provide novel insight into the function of BIK1 in leaf and inflorescence development.

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Proteolytic Processing of SERK3/BAK1 Regulates Plant Immunity, Development, and Cell Death

Abstract: Arabidopsis BAK1, a co-receptor of multiple receptor-like kinases undergoes proteolytic cleavage process, which is essential for its functions in plant immunity, growth and cell death control.

Cited by 50 publications

References 86 publications

Plant immune signaling: Advancing on two frontiers

Plant immune signaling: Advancing on two frontiers

Tyrosine phosphorylation of the lectin receptor‐like kinase LORE regulates plant immunity

BIK1 and ERECTA Play Opposing Roles in Both Leaf and Inflorescence Development in Arabidopsis

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