Proteolytic mechanisms in necrotic cell death and neurodegeneration

Artal‐Sanz, Marta; Tavernarakis, Nektarios

doi:10.1016/j.febslet.2005.03.052

Cited by 123 publications

(82 citation statements)

References 130 publications

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“…97 It was also observed that a fetal mouse heart kept for 1 hr in organ culture undergoes autophagy followed by progressive necrosis. 98,99 This confirms that autophagy acts as the first line of defense during a stress response.…”

Section: Table 1 Suggestions Of Kinetic Parameters To Be Acquired Forsupporting

confidence: 62%

Cell death: A dynamic response concept

Loos

Engelbrecht²

2009

Autophagy

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Autophagy, apoptosis and necrosis have previously been described as distinct static processes that induce and execute cell death. Due to an increased use of novel techniques in mapping cellular death-techniques which allow for reporting of real-time data-the existence of "grey zones" between cell death modes and the existence of the "point of no return" within these have been revealed. This revelation demands the integration of new concepts in describing the cellular death process. Furthermore, since the contribution of autophagy in cell death or cell survival is still poorly understood, it is important to accurately describe its function within the dynamic framework of cell death.In this review cell death is viewed as a dynamic and integrative cellular response to ensure the highest likelihood of self-preservation. Suggestions are offered for conceptualizing cell death modes and their morphological features, both individually and in relation to one another. It addresses the need for distinguishing between dying cells and dead cells so as to better locate and control the onset of cell death. Most importantly, the fundamental role of autophagy, autophagic flux, and the effects of the intracellular metabolic environment on the kinetics of the cell death modes are stressed. It also contextualizes the kinetic dimension of cell death as a process and aims to contribute towards a better understanding of autophagy as a key mechanism within this process. Understanding the dynamic nature of the cell death process and autophagy's central role can reveal new insight for therapeutic intervention in preventing cell death.

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Section: Table 1 Suggestions Of Kinetic Parameters To Be Acquired Forsupporting

confidence: 62%

Cell death: A dynamic response concept

Loos

Engelbrecht²

2009

Autophagy

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“…Necrosis cannot be discounted easily because Annexin V staining 33 and TUNEL 34 can be observed in necrotic cells. More research focusing on the proteases involved 35 might allow a molecular test for necrosis in nurse cells.…”

Section: Mechanism Of Nurse Cell Deathmentioning

confidence: 99%

Illuminating the role of caspases during Drosophila oogenesis

Mazzalupo

Cooley

2006

Cell Death Differ

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Cell death is a prominent feature of animal germline development. In Drosophila, the death of 15 nurse cells is linked to the development of each oocyte. In addition, females respond to poor environmental conditions by inducing egg chamber death prior to yolk uptake by the oocyte. To study these two forms of cell death, we analyzed caspase activity in the germline by expressing a transgene encoding a caspase cleavage site flanked by cyan fluorescent protein and yellow fluorescent protein. When expressed in ovaries undergoing starvation-induced apoptosis, this construct was an accurate reporter of caspase activity. However, dying nurse cells at the end of normal oogenesis showed no evidence of cytoplasmic caspase activity. Furthermore, although expression of the caspase inhibitors p35 or Drosophila inhibitor of apoptosis protein 1 blocked starvation-induced death, it did not affect normal nurse cell death or overall oogenesis in well-fed females. Our data suggest that caspases play no role in developmentally programmed nurse cell death.

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“…These data on the modulated expression of OKL38, BIM, HRK/BH3 and BBC3 genes taken together, suggest that initiation of the apoptotic process by Taheebo is in part due to reduced function of the anti-apoptotic BCl-2 gene. In this context it is also noteworthy that consistent with the presence of necrotic cells in Taheebo treated cultures, the Calpain family genes (21) were up-regulated to >1.5-fold, relative to those in untreated controls.…”

Section: A ----------------------------------------------------------mentioning

confidence: 90%

Growth inhibition of estrogen receptor positive human breast cancer cells by Taheebo from the inner bark of Tabebuia avellandae tree

Mukherjee

Telang²,

Wong³

2009

Int J Mol Med

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Abstract. Selective estrogen receptor (ER) modulators are used as a therapy for ER + clinical breast cancer, but they exhibit adverse effects. Herbal medicines may provide an alternative or complementary approach. Taheebo, extracted from the inner bark of the Tabebuia avellandae tree found in the Brazilian Amazon, exhibits selective anti-proliferative effects in carcinoma cell lines. The present study identifies the mechanistic leads for the inhibitory effects of Taheebo. Human breast carcinoma derived ER + MCF-7 cells were used as the model. Aqueous extract of Taheebo was the test compound. Cell cycle analysis, clonogenic assay, and global gene expression profiles were the quantitative parameters. Taheebo treatment resulted in a dose/time-dependent growth inhibition (S phase arrest, reduced clonogenecity) and initiation of apoptosis (chromatin condensation). A 6-h treatment with 1.5 mg/ml Taheebo modulated the gene expression of G2 specific cyclin B1 (-2.0-fold); S phase specific PCNA (-2.0-fold) and OKL38 (+11.0-fold); apoptosis specific GADD-45 family (+1.9-5.4-fold), Caspases (+1.6-1.7-fold), BCL-2 family (-1.5-2.5-fold), estrogen responsive ESR1 (-1.5-fold), and xeno-biotic metabolism specific CYP 1A1 (+19.8 fold) and CYP 1B1 (+7.9-fold). The anti-proliferative effects of Taheebo correlate with down-regulated cell cycle regulatory and estrogen responsive genes, and up-regulated apoptosis specific and xeno-biotic metabolism specific genes. These data validate a rapid mechanistic approach to prioritize efficacious herbal medicines, thereby complementing the existing endocrine therapy for breast cancer.

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Proteolytic mechanisms in necrotic cell death and neurodegeneration

Cited by 123 publications

References 130 publications

Cell death: A dynamic response concept

Cell death: A dynamic response concept

Illuminating the role of caspases during Drosophila oogenesis

Growth inhibition of estrogen receptor positive human breast cancer cells by Taheebo from the inner bark of Tabebuia avellandae tree

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