Proteolytic maturation of insulin is a post-Golgi event which occurs in acidifying clathrin-coated secretory vesicles

Orci, Lelio; Ravazzola, Mariella; Storch, M. J.; Anderson, Ronald Gordon; Vassalli, Jean‐Dominique; Perrelet, Alain

doi:10.1016/0092-8674(87)90624-6

Cited by 333 publications

(205 citation statements)

References 11 publications

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“…Typical mature granules contained a dense core with a halo; immature granules were fully filled with homogeneous and much lighter electron density and had tight-fitting membrane (Supplemental Figure 6). These observations were consistent with previous immuno-EM studies (52,53). The immature granules were more frequently observed in Dnm2 KO β cells (Supplemental Figure 5B), and their density was slightly higher than that in controls ( Figure 5E, P < 0.05, 2-tailed t test).…”

Section: Dynamin 2 Regulates Insulin Secretion With Normal Intracellularsupporting

confidence: 81%

Dynamin 2 regulates biphasic insulin secretion and plasma glucose homeostasis

Fan¹,

Chen²,

Wu³

et al. 2015

Journal of Clinical Investigation

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Section: Dynamin 2 Regulates Insulin Secretion With Normal Intracellularsupporting

confidence: 81%

Dynamin 2 regulates biphasic insulin secretion and plasma glucose homeostasis

Fan¹,

Chen²,

Wu³

et al. 2015

Journal of Clinical Investigation

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“…The acquisition of fusion activity may therefore also depend on the proteolytic cleavage of the prM protein. The latter occurs after a pair of basic amino acids (Rice et al, 1986) and probably is performed by a trypsin-like protease with the physiological role of processing prohormones during exocytosis (Orci et al, 1987). By recognizing the consensus sequence Arg/Lys-X-Arg/Lys-Arg the same enzyme appears to be responsible for the cleavage and activation of the fusogenic potential of a number of viral envelope glycoproteins (Strauss et al, 1987).…”

Section: Discussionmentioning

confidence: 99%

Fusion activity of flaviviruses: comparison of mature and immature (prM-containing) tick-borne encephalitis virions

Guirakhoo

Heinz²,

Mandl³

et al. 1991

Journal of General Virology

170

156

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The fusion activity of flaviviruses [tick-borne encephalitis (TBE) virus and Japanese encephalitis virus] was assessed by inducing fusion from without of C6/36 mosquito cells with purified virus preparations. Membrane fusion and polykaryocyte formation was observed only after incubating the viruses at acidic pH. Two groups of monoclonal antibodies reacting with distinct non-overlapping antigenic domains on the TBE virus protein E inhibited fusion from without. One of these domains contains the most highly conserved and putative fusion-active sequence of the flavivirus protein E. Of five TBE virus monoclonal antibody escape mutants, each defined by a single amino acid substitution in the envelope protein E, one revealed a reduced fusion activity and another one a lower pH threshold. TBE virus grown in the presence of ammonium chloride as well as Langat virus purified from the supernatant of infected chick embryo cells contained the precursor of protein M (prM) rather than M itself. These 'immature' virions did not cause fusion from without, suggesting that the proteolytic processing of prM may be necessary for the generation of fusion-competent virions.

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“…Immature and mature secretory granules Those regions of the TGN destined to form granules, as well as the earliest, immature form of the secretory granule of the regulated pathway, are coated with clathrin [55][56][57][58][59][60][61]. Clathrin [62] has been implicated in secretory, endocytotic and lysosomal trafficking pathways, although its precise role remains obscure [63][64][65][66].…”

Section: Protein Secretory Pathways: a General Outline Leader And Leamentioning

confidence: 99%