Proteolytic enzymes: a new treatment strategy for prosthetic infections?

Selan, Laura; Berlutti, Francesca; Passariello, Claudio; Comodi-Ballanti, M. R.; Thaller, María Cristina

doi:10.1128/aac.37.12.2618

Cited by 80 publications

(51 citation statements)

References 17 publications

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“…A great effort has been made to develop antipathogenic drugs and strategies (29,57,61), especially by means of reducing bacterial virulence through intercellular communication (QS). Blocking of QS in P. aeruginosa by the use of QSIs has been shown to be a promising strategy for the treatment of infections (29,49), and a series of QSIs has been identified by different groups through traditional methods (29,42,50,62).…”

Section: Discussionmentioning

confidence: 99%

Computer-Aided Identification of Recognized Drugs as Pseudomonas aeruginosa Quorum-Sensing Inhibitors

Yang

Rybtke

Jakobsen

et al. 2009

Antimicrob Agents Chemother

210

173

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Attenuation of Pseudomonas aeruginosa virulence by the use of small-molecule quorum-sensing inhibitors (referred to as the antipathogenic drug principle) is likely to play a role in future treatment strategies for chronic infections. In this study, structure-based virtual screening was used in a search for putative quorumsensing inhibitors from a database comprising approved drugs and natural compounds. The database was built from compounds which showed structural similarities to previously reported quorum-sensing inhibitors, the ligand of the P. aeruginosa quorum-sensing receptor LasR, and a quorum-sensing receptor agonist. Six top-ranking compounds, all recognized drugs, were identified and tested for quorum-sensing-inhibitory activity. Three compounds, salicylic acid, nifuroxazide, and chlorzoxazone, showed significant inhibition of quorumsensing-regulated gene expression and related phenotypes in a dose-dependent manner. These results suggest that the identified compounds have the potential to be used as antipathogenic drugs. Furthermore, the results indicate that structure-based virtual screening is an efficient tool in the search for novel compounds to combat bacterial infections.

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Section: Discussionmentioning

confidence: 99%

Computer-Aided Identification of Recognized Drugs as Pseudomonas aeruginosa Quorum-Sensing Inhibitors

Yang

Rybtke

Jakobsen

et al. 2009

Antimicrob Agents Chemother

210

173

View full text Add to dashboard Cite

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“…Chaignon et al (2007) showed that the enzymatic detachment of staphylococcal biofilms depends on the nature of their constituents and varies between clinical isolates; they tested the effect of different enzymes on biofilms and suggested a complex mixture of dispersin B and a protease (like trypsin) to remove the biofilms of a variety of staphylococcal strains. Other in vitro studies showed that proteolytic enzymes (Selan et al 1993) or lysostaphin (Walencka et al 2005;Walencka et al 2006) could significantly enhance the activities of antibiotics against biofilms.…”

Section: Discussionmentioning

confidence: 99%

“…This probably suggests that enzymes increase the cure rate by breaking down pus, clot and fibrin and by clearing the milk duct and without any effect on biofilm. Some investigators showed that the use of enzyme is not useful in the planktonic condition of growth (Selan et al 1993;Johansen et al 1997;Berg et al 2001;Kaplan et al 2004). We evaluated biofilm formation capacity outside of the mammary gland; obviously, our in vitro conditions may differ from the in vivo situation.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic effects of a combined antibiotic-enzyme treatment on subclinical mastitis in lactating dairy cows

Khoramian¹,

Emaneini²,

Bolourchi³

et al. 2016

Vet. Med. - Czech

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ABSTRACT:The objective of this study was to evaluate a combined antibiotic-enzyme therapy for Staphylococcus aureus mastitis and biofilm formation. A total of 141 cases of S. aureus chronic mastitis from three farms were divided into two groups: the control group (n = 54) were treated with Nafpenzal ® ointment; the enzyme + antibiotic group (n = 87) were treated with Nafpenzal ® plus an enzymatic ointment (MastiVeyxym ® ). Quantitative determination of biofilm formation was determined using a colorimetric microplate assay and the detection of ica genes by PCR. Enzyme + antibiotic therapy did not significantly improve cure rates compared to control (48.3 vs 38.9%). The cure rate for infections caused by biofilm-positive strains was 42.6 and 46.6% for control and enzyme + antibiotic groups, respectively (P > 0.05). Comparison of cure rates between farms showed a relationship with somatic cell count (SCC), parity and oxacillin resistance. 79.4% of the isolates produced biofilm and antibacterial resistance rates for oxacillin, penicillin and streptomycin were 25.5, 71.6 and 95.7%, respectively. These results indicate that while the increase in mastitis cure rate using enzymatic therapy was not significant, this treatment could be useful in some situations.

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“…We assayed seminal plasma 90K/MAC-2BP in 50 infertile male patients in whom diagnosis of male accessory gland infection was made according to standardized criteria and in 30 controls without male accessory gland infection and with normal sperm parameters. Assays were also performed in patients after 1-6 cycles of treatment with levofloxacin 500 mg, a fluoroquinolone whose efficacy on microbiologic eradication rates is known (Bundrick et al, 2003), plus serratiopeptidase, a 50.6-kd metalloprotease that demonstrates strong proteolytic activity enhancing the penetration of antibiotics in infected sites (Yamazaki et al, 1967) and enhancing the activity of quinolones against the development of bacterial biofilms (Selan et al, 1993), while in controls seminal 90K was reevaluated after 2 months.…”

Section: Discussionmentioning

confidence: 99%

Detection of 90K/MAC‐2BP in the Seminal Plasma of Infertile Males With Accessory Gland Infection and the Autoimmune Pathogenetic Hypothesis

Caroppo

Niederberger

Iacovazzi

et al. 2006

Journal of Andrology

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The purpose of the study was to evaluate 90K/MAC-2BP, a glycoprotein member of the Scavenger Receptor Cystein Rich superfamily, in the seminal plasma of infertile male patients with male accessory gland infection in order to investigate a putative autoimmune pathogenesis. 90K seminal concentration and sperm parameters were evaluated in 50 patients with male accessory gland infection at baseline and after cycles of treatment with Levofluoxacin 500 mg daily for 15 days plus serratiopeptidase 10 mg daily for 30 days. Treatment was continued for up to 6 cycles in cases of persistant bacteriospermia and/or clinical and ejaculatory signs of the disease. Patients with persistant male accessory gland infection after 6 cycles were defined as nonresponders. The same parameters were evaluated at baseline and after a 2-month period in 30 healthy controls. Patients with male accessory gland infection showed impaired sperm parameters and had lower seminal 90K concentration compared to controls. After treatment, seminal 90K level significantly increased in patients compared to controls. Twenty-two patients responded to treatment (44%), while 28 were nonresponders (56%). No difference in pretreatment and posttreatment sperm parameters and seminal 90K was observed between the 2 subgroups. Thirteen patients (26%) had identifiable bacteriospermia: significantly less pretreatment seminal 90K was observed compared to patients without bacteriospermia. Seminal 90K is decreased in patients with male accessory gland infection, and may be restored by a treatment with quinolones. However, the clinical utility of a 90K assay in these patients remains uncertain, as its level is not predictive of response to treatment.

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Proteolytic enzymes: a new treatment strategy for prosthetic infections?

Cited by 80 publications

References 17 publications

Computer-Aided Identification of Recognized Drugs as Pseudomonas aeruginosa Quorum-Sensing Inhibitors

Computer-Aided Identification of Recognized Drugs as Pseudomonas aeruginosa Quorum-Sensing Inhibitors

Therapeutic effects of a combined antibiotic-enzyme treatment on subclinical mastitis in lactating dairy cows

Detection of 90K/MAC‐2BP in the Seminal Plasma of Infertile Males With Accessory Gland Infection and the Autoimmune Pathogenetic Hypothesis

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