Proteolytic activity of wild fruits of Bromelia karatas L. of Yucatán, Mexico

Villanueva-Alonzo, Hernán de Jesús; Polanco-Hernández, Glendy; Lizama-Uc, Gabriel; Acosta-Viana, Karla Y.; Alvarado-Segura, Arturo A.

doi:10.5154/r.rchscfa.2018.08.062

Cited by 3 publications

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“…We determined the bactericidal activity of the B. karatas fruits' methanolic extract (ME) against E. coli, S. enteritidis, S. flexneri, and E. faecalis (causal agents of human infections) [5,20]; it was shown that these results are consistent with those reported in Bromelia pinguin [6]. We found that the bactericidal activity resisted the sterilization temperature, meaning its potential benefits are still maintained when the fruits are consumed cooked since cooking is necessary to avoid oral injuries caused by their proteolytic activity [11]. In another study, it was determined that the protein extract of the fruits had bactericidal activity, but this was not resistant to heat [14,15], which differs from the heat-resistant bactericidal activity of the methanolic extract found in the present study.…”

Section: Discussionmentioning

confidence: 65%

“…The genus Bromelia includes species that grow wild in Mexico and their fruits are known for their antifungal [7], bactericidal [6,8], and anthelmintic [9] activity. Bromelia karatas fruits contain antioxidants, proteases, and phenolic compounds, such as flavonoids, phenylpropanoids, terpenes, and coumarins [10,11]. Throughout tropical America, B. karatas fruits are consumed as foods and beverages, and their stems can be used as living fences.…”

Section: Introductionmentioning

confidence: 99%

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Compound Identification from Bromelia karatas Fruit Juice Using Gas Chromatography–Mass Spectrometry and Evaluation of the Bactericidal Activity of the Extract

et al. 2022

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Fruits of species of the genus Bromelia contain compounds with health benefits and potential biotechnological applications. For example, Bromelia karatas fruits contain antioxidants and proteins with bactericidal activity, but studies regarding the activity of these metabolites and potential benefits are required. We evaluated the bactericidal activity of the methanolic extract (treated and not treated with activated charcoal) and its fractions (hexane, ethyl acetate, and methanol) from ripe B. karatas fruit (8 °Brix) against Escherichia coli, Enterococcus faecalis, Salmonella enteritidis, and Shigella flexneri. The methanolic extract (ME) minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined at eight concentrations. The methanolic extract MIC was 5 mg/mL for E. faecalis and 10 mg/mL for the other bacteria; the MBC was 20 mg/mL for E. coli and E. faecalis, and 40 mg/mL for S. enteritidis and S. flexneri. Through gas chromatography–mass spectrometry, 131 compounds were identified, some of which had previously been reported to have biological activities, such as bactericidal, fungicide, anticancer, anti-inflammatory, enzyme inhibiting, and anti-allergic properties. The most abundant compounds found in the ME of B. karatas fruits were maleic anhydride, 5-hydroxymethylfurfural, and itaconic anhydride. This study shows that B. karatas fruits contain metabolites that are potentially beneficial for health.

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Section: Discussionmentioning

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Compound Identification from Bromelia karatas Fruit Juice Using Gas Chromatography–Mass Spectrometry and Evaluation of the Bactericidal Activity of the Extract

et al. 2022

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“…The obtaining of new sources of plant proteases is increasing due to their low production costs, relative abundance in some plants and attractive biochemical characteristics [25], such as their stability over a wide range of pH and temperature [26]. The fruits of Bromelia pinguin "guamara" and Bromelia karatas "cocuixtle" are presented as an alternative to obtaining plant proteases [4,[26][27][28][29]. However, little information exists on the application of these sources of proteases in different food matrices to obtain protein hydrolysates.…”

Section: Introductionmentioning

confidence: 99%

Bromelia karatas and Bromelia pinguin: sources of plant proteases used for obtaining antioxidant hydrolysates from chicken and fish by-products

et al. 2020

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In the present study, we evaluated new sources of plant proteases from fruits of Bromelia karatas (BK) and Bromelia pinguin (BP) to obtain antioxidant hydrolyzates/bioactive peptides (BPs) derived from chicken by-products (CH) and fish by-products (FH). The profile of the peptides was identified by reverse-phase high-resolution liquid chromatography (RP-HPLC) and the size weight distribution by molecular exclusion chromatography (SEC). The hydrolysates obtained with BK in both sources of by-products showed greater antioxidant capacity compared to those obtained with BP, presenting similar or higher values when compared to a commercial plant enzyme. The use of new sources of plant proteases allowed to obtain hydrolysates of hydrophilic character with a high percentage (> 50%) of peptides with molecular weights < 17.5 kDa from chicken and fish by-products. Therefore, based on the results obtained in antioxidant capacity it is possible to consider the hydrolysates as potential ingredients, food additives, and pharmaceutical products.

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Proteolytic characterization of a novel enzymatic extract from Bromelia serra leaves

Herrera

Luaces

Liggieri³

et al. 2022

An. Acad. Bras. Ciênc.

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Bromelia serra leaves collected from Corrientes, Argentina, were assessed to analyze and characterize the proteolytic system and to evaluate its potential use as an industrial catalyst. The specifi c activity of the enzymatic extract (EE), which was prepared using acetone as a precipitating agent of the crude extract (CE), increased 2-3 folds with different substrates (hemoglobin, azocasein and casein). The proteins present in the EE have isoelectric points between 4.55-8.15 and they were signifi cant inhibited by pepstatin A (50%) and E-64 (15%). Proteolytic activity in EE presented high activity in acidic pH (2.7-4), and low activity in neutral alkaline pH (6-11.75). The EE optimum activity was reached at 60ºC, and referring to the thermal stability, it retained over 97% of the proteolytic activity after incubation at a temperature range of 37-60 ºC for 60 min. The effect of reducing agents and ionic strength were also measured, and it showed that the EE had its maximum activity with 5mM of cysteine, and it was inactivated with 2.5 M of NaCl. The chromatography procedures presented two purifi ed enzymes of 21 and 54 KDa with proteolytic activity. The characteristics of the EE suggest that it is a potential candidate as an industrial catalyst.

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Proteolytic activity of wild fruits of Bromelia karatas L. of Yucatán, Mexico

Cited by 3 publications

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Compound Identification from Bromelia karatas Fruit Juice Using Gas Chromatography–Mass Spectrometry and Evaluation of the Bactericidal Activity of the Extract

Compound Identification from Bromelia karatas Fruit Juice Using Gas Chromatography–Mass Spectrometry and Evaluation of the Bactericidal Activity of the Extract

Bromelia karatas and Bromelia pinguin: sources of plant proteases used for obtaining antioxidant hydrolysates from chicken and fish by-products

Proteolytic characterization of a novel enzymatic extract from Bromelia serra leaves

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