Proteolytic Activity of the Roach (Rutilus RutilusL.) Intestinal Microflora

Abstract

For research, two differently polluted lakes were selected: Lake Dringis, Aukðtaitija National Park, as an ecosystem pattern of a weak anthropogenic influence, and Lake Drûkðiai, as a regularly polluted water body. Lake Drûkðiai, the cooling basin of Ignalina Nuclear Power Plant, through its tributaries is being polluted with industrial and municipal sewage. Samples were taken from four differently polluted mouths of tributaries: the mouth of the Rièanka Stream; the mouth of the Gulbinës Stream affected by urban rain sewerage of Visaginas (URS-2); the mouth of the Gulbinëlë Stream into which municipal sewage of Visaginas (MS) and industrial rain sewerage from INPP (IRS-1.2) flow. The work was aimed to determine the role of the symbiotic bacterioflora of the roach digestive tract in protein decomposition, as well as the dependence of proteolytic enzyme activity upon environmental pollution. Comparison of the proteolytic activity (PA) of the roach intestinal bacterioflora showed reliable differences between Lakes Dringis and Drûkðiai. The total mean PA of bacterioflora in Lake Dringis roach intestines was established to be twice higher than that in Drûkðiai. At the URS-2, MS, and IRS-1.2 mouths of Lake Drûkðiai, the mean PAs of the isolates were reliably similar. The mean PA was a little higher in the Rièankas mouth roach intestinal bacteria. The highest mean PA in active strains as well as the highest total mean PA was typical of Lake Dringis roach intestinal strains. In Lake Dringis, the highest mean PA in active bacteria strains was determined in the IRS-1.2 mouth roach intestines. The research revealed the enzyme activity in fish intestinal bacterioflora to be dependent upon the ecology state of environment.

Microflora in fish digestive tract plays significant role in digestion and metabolism

“…Investigation results suggest that bacteria of the genera Aeromonas, Pseudomonas and Flavobacterium-Cytophaga prevail in the bacteriocenoses of the digestive tract of freshwater fish (Ringø and Birkbeck 1999; Vesta Skrodenyte-Arbaeiauskiene 2000; Voveriene et al 2002). The impact of feeding intensity and food on the qualitative and quantitative composition of intestinal bacteriocenoses of fish was studied by a number of scientists (Ringo and Olsen 1999;Pucci et al 2004;Vesta Skrodenyte-Arbaeiauskiene 2000;Voveriene et al 2002). The structure of intestinal bacteriocenoses of fish is influenced by farming conditions of fish, too (Ringo and Strom 1994).…”

Role of gastrointestinal microbiota in fish

“…The GI microbiota of hydrobionts has been reported to contribute to the nutrition and physiological processes of the host by producing vitamins, amino acids, digestive enzymes and metabolites, similar to that of mammals (Syvokiene 1989; Cahill 1990; Sugita et al 1990; Sugita, Matsuo, Hirose, Iwato & Deguchi 1997; Mickeniene 1999; Skrodenyte‐Arbaeiauskiene 2000; Skrodenyte‐Arbaeiauskiene et al 2006). Nevertheless, a wide range of enzymes like carbohydrases, phosphatases, esterases, lipases and peptidases, cellulase, lipase and proteases (Bairagi et al 2002; Ramirez & Dixon 2003; Izvekova & Lapteva 2004) produced by GI bacteria could be a contributory source to digestive enzymes in fish.…”

“…; Sugita, Matsuo, Hirose, Iwato & Deguchi 1997;Mickeniene 1999;Skrodenyte- Arbaeiauskiene 2000; Skrodenyte-Arbaeiauskiene et al 2006). Nevertheless, a wide range of enzymes like carbohydrases, phosphatases, esterases, lipases and peptidases, cellulase, lipase and proteases(Bairagi et al 2002;Ramirez & Dixon 2003;Izvekova & Lapteva 2004) produced by GI bacteria could be a contributory source to digestive enzymes in ¢sh.…”

Characterization of the gut microbiota in the Anjak (Schizocypris altidorsalis), a native fish species of Iran

“…are commensal a number are major pathogens of freshwater and marine fishes (Loch and Faisal 2015). It has been suggested that fish gut isolated F lavobacterium sp might be possible contributors to the digestive processes of fish by producing enzymes such as amylase (Skrodenytė-Arbačiauskienė 2000), chitinase (Ray et al 2012) and protease (Morita et al 1998; Skrodenytė-Arbačiauskienė 2000).…”