Proteolytic Activity of IGFBP-3 in Various Clinical Conditions During Childhood Studied by Means of Western Immunoblotting.

Hasegawa, Yukihiro; Hasegawa, Tomonobu; Koni, Hideko; Aso, Taiji; Tanaka, Noriko; Kotoh, Shinobu; Yamada, Makoto; Tsuchiya, Yutaka

doi:10.1507/endocrj.42.569

Cited by 13 publications

(15 citation statements)

References 22 publications

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“…Each sample (0.002 ml) was subjected to SDS-PAGE (12%) directly or after incubation with 0.002 ml of adult (nonpregnant) serum at 37 °C for 2 h. The gel was transferred to nitrocellulose membrane, which was incubated with the anti-IGFBP-3 antibody and then the HRP-linked antibody (rat anti-rabbit gammaglobulin antibody). as previously described [13]. In pregnant sera, the activities were generally more than 50%.…”

Section: Subjectsmentioning

confidence: 77%

“…Here the levels of fIGF-I and tIGF-I were measured by immunoradiometric assay (IRMA) [12], and the proteolysis of IGFBP-3 was analyzed by Western immunoblotting [13] in the circulation of patients with precocious puberty and normal children who showed signs of a pubertal growth spurt.…”

Section: In the Circulationmentioning

confidence: 99%

“…Western immunoblotting IGFBP-3 proteolytic activity was measured by Western immunoblotting [13]. Each sample (0.002 ml) was subjected to SDS-PAGE (12%) directly or after incubation with 0.002 ml of adult (nonpregnant) serum at 37 °C for 2 h. The gel was transferred to nitrocellulose membrane, which was incubated with the anti-IGFBP-3 antibody and then the HRP-linked antibody (rat anti-rabbit gammaglobulin antibody).…”

Section: Subjectsmentioning

confidence: 99%

“…In normal control children (age, 4-7 years) and adults (age, 30-36 years), the activities were less than 10% in all cases studied (N=18). In this paper, less than 10% activity is called no activity [13].…”

Section: Subjectsmentioning

confidence: 99%

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Increase in Free Insulin-Like Growth Factor-I Levels in Precocious and Normal Puberty.

Yamada

Hasegawa

1998

Endocr J

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Abstract. Previously we reported a high ratio of free insulin-like growth factor-I (IGF-I) to total IGF-I (fit IGF-I ratio) during early infancy when rapid growth is observed, indicating that high levels of free IGF-I (fIGF-I) may be related to the growth of infants. Rapid growth is also observed during puberty. The purpose of this study is to investigate a possible relationship between pubertal growth and fIGF-I. Here, fIGF-I and total IGF-I (tIGF-I), the f /t IGF-I ratio and proteolysis of IGFBP-3 were studied in the circulation of patients with precocious puberty and normal pubertal subjects, and they were compared with those of prepubertal and adult populations. Higher absolute plasma fIGF-I values were observed in the pubertal children (3.89 ± 1.09 ng/ml, N=45) than in the normal prepubertal children (2.06 ± 1.01 ng/ml, N=31; P<0.05) and adults (2.18 ± 0.68 ng/ml, N=49; P<0.05). Furthermore, there was a correlation between the levels of fIGF-I and height velocity in the prepubertal and pubertal groups (r=0.722, N=76, P<0.0001). Compared to the f/t IGF-I in the prepubertal and adult populations, no increase in fit IGF-I ratios was observed, and the proteolysis of IGFBP-3 was not detected in sera from the pubertal children. These data suggest that the increase in the absolute levels of fIGF-I is related to pubertal growth.

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Section: Subjectsmentioning

confidence: 77%

Section: In the Circulationmentioning

confidence: 99%

Section: Subjectsmentioning

confidence: 99%

Section: Subjectsmentioning

confidence: 99%

See 2 more Smart Citations

Increase in Free Insulin-Like Growth Factor-I Levels in Precocious and Normal Puberty.

Yamada

Hasegawa

1998

Endocr J

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“…Therefore, the concentration of IGFBPs in the circulation might be influence by the production of the IGFBPs from fibroblasts. Serum IGFBP-3 levels have been shown to decease in several situations such as surgery, pregnancy, and infection [9][10][11]. The reduction of IGFBP-3 in these situations are, at least in part, due to proteolytic degradation of IGFBP-3.…”

Section: Resultsmentioning

confidence: 99%

Effect of Cytokines on Production of Insulin-like Growth Factor Binding Proteins (IGFBPs) from Human Fibroblasts in Culture

et al. 1999

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Clinical information on serum IGFBP-3 levels and IGFBP-3 proteolytic activity in childhood

Hasegawa

Fujii

et al. 1995

Progress in Growth Factor Research

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Proteolytic Activity of IGFBP-3 in Various Clinical Conditions During Childhood Studied by Means of Western Immunoblotting.

Cited by 13 publications

References 22 publications

Increase in Free Insulin-Like Growth Factor-I Levels in Precocious and Normal Puberty.

Increase in Free Insulin-Like Growth Factor-I Levels in Precocious and Normal Puberty.

Effect of Cytokines on Production of Insulin-like Growth Factor Binding Proteins (IGFBPs) from Human Fibroblasts in Culture

Clinical information on serum IGFBP-3 levels and IGFBP-3 proteolytic activity in childhood

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